BIST™ – a New Mode of LC Separation
Bridge Ion Separation Technology, or BIST™, is a brand new LC separation technique developed by SIELC. The goal of BIST™ is simple – expand the scope of HPLC applications.
SIELC Technologies’ BIST™ is a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! In short, BIST™ is complex chromatography without the hassle.
BIST™ provides an additional separation tool to a whole class of organic and inorganic charged molecules. BIST™ columns, simple mobile phases, and an array of applications provides a whole new continent on the chromatography map.
Analyze Ions Differently
When doubly-charged ions are present in the mobile phase (MP), the surface of the stationary phase can switch its polarity.
For example: if the surface is positively-charged and the doubly-charged ions in the mobile phase are sulfate ions with a
minus 2 charge (from ionized sulfuric acid), then the surface can became negatively-charged.
However, this can occur only when the mobile phase has a relatively low concentration of water. When water is
the main component of the mobile phase, it forms a solvation shell around each ion and prevents the surface
charge from switching.
The same surface switch is observed when a negatively-charged surface interacts with doubly-charged
positive ions such as diamines, and some inorganic ions such as Mg2+ and Ca2+.
BIST™ Products
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BIST A
BIST A is a silica-based cation-exchange column (negatively-charged surface) with hydrophilic properties. Compatible with LC-MS and preparative chromatography. BIST™ (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple negative charges. BIST™ is a simple way to achieve retention and elution plus an effective separation that are traditionally difficult or impossible to achieve with any other HPLC columns. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC. Select options -
BIST A+
BIST A+ is a silica-based cation-exchange column (negatively-charged surface) with hydrophilic properties. Compatible with LC-MS and preparative chromatography. BIST A+ provides more retention at similar conditions for negatively charged molecules if compared to BIST A. Alternatively, it can be used with lower concentration of the organic modifier in the mobile phase in order to get the same result as BIST A. BIST™ (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple negative charges. BIST™ is a simple way to achieve retention and elution plus an effective separation that are traditionally difficult or impossible to achieve with any other HPLC columns. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC. Select options -
BIST AC
BIST AC is a silica-based cation-exchange column (negatively-charged surface) with hydrophilic properties. Compatible with LC-MS and preparative chromatography. BIST AC is specifically developed for separation of oligonucleotides. It provides alternative separation conditions, selectivity and mobile phase if compared with traditionally used reverse phase and ion-exchange separation of oligonucleotides. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC or go to https://sielc.com/oligos Select options -
BIST B
BIST B is a positively charged silica-based anion-exchange column with hydrophilic properties. Compatible with LC-MS and preparative chromatography. It is the preferred column when molecules with a very high number of positive charges need to be analyzed. Examples of such compounds are polyimine, polylysine, glucosamines. BIST™ (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple negative charges. BIST™ is a simple way to achieve retention and elution plus an effective separation that are traditionally difficult or impossible to achieve with any other HPLC columns. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC. Select options -
BIST B+
BIST B+ is a positively charged silica-based anion-exchange column with hydrophilic properties. Compatible with LC-MS and preparative chromatography. BIST B+ provides more retention at similar conditions for positively charged molecules if compared to BIST B. Alternatively, it can be used with lower concentration of the organic modifier in the mobile phase to achieve results comparable to BIST B. BIST™ (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple negative charges. BIST™ is a simple way to achieve retention and elution plus an effective separation that are traditionally difficult or impossible to achieve with any other HPLC columns. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC. Select options
For more information and a complete look at BIST™, please check out our other BIST™ pages and our brochure below!
BIST™ Brochure
The Theory Behind BIST™
BIST™ Applications
BIST Ionic Modifier Preparation
Copyright © 2022 by SIELC Technologies Corporation.
The information and disclosures contained herein were originated by and are the property of SIELC Technologies Corporation and may not be copied, distributed, republished, translated, uploaded, posted, transmitted, stored, or otherwise used in any shape, manner, or form by any means without the specific prior written permission of SIELC Technologies Corporation. Modification or use of this material for any reason shall be construed as a violation of the property rights of SIELC Technologies Corporation. SIELC Technologies Corporation reserves all rights, including without limitation all patent, proprietary design, manufacturing, reproduction, use, advertising, and sales rights thereto, and to any articles disclosed herein, except to the extent such rights are granted to others by law or by SIELC Technologies Corporation. BISTTM is a registered trademark of SIELC Technologies Corporation. U.S. Patents Pending. All data were obtained in SIELC Technologies labs.
