Bromadiolone a rodenticide meant to kill rats and mice. It’s sold as an odorless powder with a light yellow tint of color. It is toxic if ingested orally since it the blood clotting due to the rodenticide not letting the body to reuse the K Vitamin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Bromadiolone. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.