Cannsep B is a reverse-phase analytical column with embedded strong basic ion-pairing groups.
This stationary phase retains neutral cannabinoids by reverse-phase mechanism.
Acidic cannabinoids such as Tetrahydrocannabinolic acid (THCA) and Cannabidiolic Acid (CBDA) will be a subject of an ion-exchange with positively charged stationary phase of Cannsep B.
Mobile phases can be selected to be compatible with low UV detection with phosphoric or sulfuric acid as a modifier. Alternately the LC-MS and preparative chromatography compatible mobile phase can be use with formic acid as an ionic modifier.