Choline

Choline structural formula

CAS Number62-49-7
Molecular FormulaC5H14NO
Molecular Weight104.172
InChI KeyOEYIOHPDSNJKLS-UHFFFAOYSA-N
LogP-2.81
Synonyms
  • Choline ion
  • 2-Hydroxy-N,N,N-trimethylethan-1-aminiumato
  • Ethanaminium, 2-hydroxy-N,N,N-trimethyl-
  • 62-49-7
  • 3-04-00-00651
  • Ethanaminium, 2-hydroxy-N,N,N-trimethyl-
  • (2-Hydroxyethyl)trimethylammonium
  • 2-Hydroxy-N,N,N-trimethylethanaminium
  • Bilineurine
  • choline
  • Choline cation
  • BRN 1736748
  • EINECS 200-535-1
  • UNII-N91BDP6H0X
  • (2-Hydroxyethyl)trimethyl ammonium
  • (2-hydroxyethyl)trimethylazanium
  • (beta-hydroxyethyl)trimethylammonium
  • 2-Hydroxy-N,N,N-trimethyl-ethanaminium
  • 2-Hydroxy-N,N,N-trimethylethanaminium
  • 2-hydroxyethyl-trimethyl-ammonium
  • 2-hydroxyethyl-trimethyl-azanium
  • Biocolina
  • Biocoline
  • Cholinum
  • Hepacholine
  • Hormocline
  • Lipotril
  • N,N,N-Trimethylethanolammonium
  • N-trimethylethanolamine
  • Neocolina
  • Paresan
  • trimethylethanolamine
  • 139741-81-4

Applications:

HPLC ELSD Method for Analysis Acetylcholine and Choline on Obelisc R Column

January 18, 2024

HPLC Method for Analysis of Acetylcholine, Choline on Obelisc R by SIELC Technologies

HPLC-ELSD-Method-for-Analysis-Acetylcholine-and-Choline-on-Obelisc-R-Column
HPLC Method for Analysis of Acetylcholine, Choline on Obelisc R Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetylcholine, Choline

Acetylcholine and choline are related compounds that play distinct roles in the body, particularly in the nervous system.

  1. Choline:
  • Definition: Choline is an essential nutrient that belongs to the B-vitamin complex.
  • Chemical Structure: Choline is a quaternary ammonium salt containing a positively charged nitrogen atom and is often written as (N(CH_3)_3^+).
  • Function: Choline serves as a precursor for the synthesis of various important molecules, including phospholipids (essential components of cell membranes) and the neurotransmitter acetylcholine. Choline is crucial for maintaining cell membrane integrity and is involved in liver function.
  • Dietary Sources: Choline is found in foods such as eggs, meat, fish, nuts, and certain vegetables. It can also be consumed as a dietary supplement.
  1. Acetylcholine:
  • Definition: Acetylcholine is a neurotransmitter, which is a chemical messenger that transmits signals between nerve cells (neurons) or from neurons to muscles.
  • Chemical Structure: Acetylcholine is derived from the combination of choline and acetyl coenzyme A. Its chemical formula is (CH_3COOCH_2CH_2N(CH_3)_3^+).
  • Function: Acetylcholine plays a crucial role in transmitting signals across synapses, including those between neurons and muscles (neuromuscular junctions). It is involved in muscle contraction, autonomic nervous system functions, and cognitive processes such as memory and learning.
  • Synthesis and Release: Acetylcholine is synthesized within nerve cells and released into synapses upon a nerve impulse. Its action is terminated by the enzyme acetylcholinesterase, which breaks it down in the synaptic cleft.

In summary, choline is a nutrient that the body uses to synthesize acetylcholine, among other important molecules. Acetylcholine, on the other hand, is a neurotransmitter responsible for transmitting signals in the nervous system. While choline is obtained from the diet or supplements, acetylcholine is a key mediator of nerve and muscle function in the body.

Acetylcholine and Choline can be retained, separated and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 60/40%
BufferAmmonium acetate pH 5.0 – 10 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 70°C,
 with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples0.5 mg/ml
Injection volume1 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Quaternity amines
Analyzing CompoundsAcetylcholine, Choline

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Acetylcholine
Choline

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC ELSD Method for Analysis of Choline on Obelisc R Column

January 15, 2024

HPLC Method for Analysis of Choline on Obelisc R by SIELC Technologies

HPLC-ELSD-Method-for-Analysis-of-Choline-on-Obelisc-R-Column
HPLC Method for Analysis of Choline on Obelisc R Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of Choline

Choline is an essential nutrient that plays a crucial role in various physiological processes in the body. It is often grouped with B-vitamins, but it is not officially recognized as a vitamin. Choline serves several important functions, including:

  1. Cell Membrane Structure: Choline is a component of phospholipids, essential structural components of cell membranes. It helps maintain the structural integrity and fluidity of cell membranes.
  2. Neurotransmitter Synthesis: Choline is a precursor to acetylcholine, a neurotransmitter that is involved in muscle control, memory, and other cognitive functions. Adequate choline levels are important for maintaining proper neurotransmitter balance in the brain.
  3. Methyl Group Donor: Choline is a source of methyl groups, which are important for various biochemical reactions in the body, including the methylation of DNA, a process that regulates gene expression.
  4. Brain Development: Choline is particularly important during periods of rapid growth, such as fetal development and early childhood. Adequate choline intake during pregnancy is associated with better cognitive outcomes in children.
  5. Liver Function: Choline is involved in lipid metabolism and helps prevent the accumulation of fat in the liver. It is essential for the transport and metabolism of fats, and it plays a role in preventing non-alcoholic fatty liver disease.
  6. Muscle Function: Choline is involved in muscle movement and helps regulate the functioning of muscles.

Food Sources:
Choline is found in a variety of foods. Some good dietary sources of choline include:

  • Eggs
  • Meat, poultry, and fish
  • Dairy products
  • Nuts and seeds
  • Certain vegetables, such as broccoli and Brussels sprouts

Choline can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD

ColumnObelisc R, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 20/80%
BufferAmmonium acetate pH 5.0 – 10 mM
Flow Rate0.2 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 70°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
Samples1 mg/ml
Injection volume1 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Quaternity amines
Analyzing CompoundsCholine

Application Column

Obelisc R

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Choline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Choline on Obelisc R Column

October 10, 2018

Separation type: Liquid Chromatography Ion Exclusion

High Performance Liquid Chromatography (HPLC) Method of Choline

Choline is a water-soluble vitamin-like essential nutrient.  It is a constituent of lecithin, which is present in many plants and animal organs.  Choline is a nutrient related to the B-vitamin family.  First discovered in 1862, it wasn’t classified as an essential nutrient until very recently.  It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties.  Choline can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by ELSD, CAD or LC/MS.

ColumnObelisc R, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 20/80%
BufferAmFm pH 4.0- 5 mM
Flow Rate1.0 ml/min
DetectionCAD (Corona) MS- compatible mobile phase
Class of Compounds
Drug,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing CompoundsCholine

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:

Choline

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Succinylcholine and Choline Using a Primesep 200 Column

July 11, 2012

 

Condition

Column Primesep 200, 4.6×50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN 0 – 20 %, 7 min
Buffer TFA – 0.05-0.20%
Flow Rate 1.0 ml/min
Detection ELSD 45C

 

Description

Class of Compounds
Drug, Acid, Paralytic, Hydrophilic, Ionizable
Analyzing Compounds Succinic Acid, Succinylcholine, Choline

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Choline
Succinic Acid
Succinylcholine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.