| CAS Number | 81-04-9 |
|---|---|
| Molecular Formula | C10H8O6S2 |
| Molecular Weight | 288.291 g/mol |
| InChI Key | XTEGVFVZDVNBPF-UHFFFAOYSA-N |
| LogP | -0.262 |
| Synonyms |
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Applications:
HILIC Retention of 1,5-Naphthalenedisulfonic Acid on Obelisc N Mixed-Mode HILIC Columns
Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.
| Column | Obelisc N , 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 1,5-Naphthalenedisulfonic Acid |
Application Analytes:
1,5-Naphthalenedisulfonic Acid
Sulfonic Acid
HPLC Separation of Organic Acids in HILIC and Anion-Exclusion Mode on Primesep S2 Column
Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).
| Column | Primesep S2, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 85/15% |
| Buffer | AmAc pH 5.0 15 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 50C UV 250 nm |
| Class of Compounds | Nucleosides, Hydrophilic, Ionizable |
| Analyzing Compounds | Toluenesulfonic acid, Naphthalenedisulfonic acid, 3.5 DHBA, Ascorbic acid, |
Application Analytes:
1,5-Naphthalenedisulfonic Acid
3,5-Dihydroxybenzoic Acid
Ascorbic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)
HPLC Determination of 1,5-Naphthalenedisulfonic Acid on Newcrom BH Column
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1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge. Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing. The acid can be retained with great peak shape on a Newcrom BH mixed-mode column. The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules. The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible. UV detection at 260nm can also be used.
| Column | Newcrom BH, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O - 20/80% |
| Buffer | Ammonium Formate - 20 mM, pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260nm |
| Class of Compounds | Acid |
| Analyzing Compounds | 1,5-Naphthalenedisulfonic Acid |
Application Analytes:
1,5-Naphthalenedisulfonic Acid