CAS Number | 3106-60-3 |
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Molecular Formula | C7H9N2O |
Molecular Weight | 137.161 g/mol |
InChI Key | LDHMAVIPBRSVRG-UHFFFAOYSA-O |
LogP | -2.06 |
Synonyms |
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A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH