1-Naphthaleneacetic acid (NAA) Separation on Primesep and Obelisc Mixed-Mode Columns

   
1-Naphthylacetic acid (NAA) is a synthetic plant hormone which is useful for promoting growth and propagating leaf and stem cuttings. Auxins can prevent early thinning and dropping of fruits from stems. But at slightly elevated levels, it can be toxic to plants and animals. A single residue method was developed using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, to be used by EURL (European Union Reference Laboratory). 1-Naphthylacetic acid was separated by both Obelisc R and Primesep 100. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. Methods are LC/MS compatible and able to be used with many pesticides.
 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 3 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Synthetic plant hormone, Acid,   Hydrophobic, Ionizable
Analyzing Compounds 1-Naphthaleneacetic acid (NAA)
 

Application Analytes:

1-Naphthylacetic acid