2,4-DB

2,4-DB structural formula

CAS Number94-82-6
Molecular FormulaC10H10Cl2O3
Molecular Weight249.090
InChI KeyYIVXMZJTEQBPQO-UHFFFAOYSA-N
LogP3.53
Synonyms
  • 2,4-DB
  • 4-(2,4-Dichlorophenoxy)butanoic acid
  • Butanoic acid, 4-(2,4-dichlorophenoxy)-
  • 94-82-6
  • 4-(2,4-Dichlorophenoxy)butyric acid
  • 4-06-00-00927
  • Butanoic acid, 4-(2,4-dichlorophenoxy)-
  • 2,4-D butyric acid
  • 2,4-Dichlorophenoxybutyric acid
  • 4-(2,4-Dichlorophenoxy)butanoic acid
  • 4-(2,4-Dichlorphenoxy)buttersaure
  • acide 4-(2,4-dichlorophenoxy)butyrique
  • acido 4-(2,4-diclorofenoxi)butirico
  • Buratal
  • Butyrac
  • Butyrac 118
  • Butyric acid, 4-(2,4-dichlorophenoxy)-
  • Legumex
  • Legumex D
  • NSC 70337
  • Sys 67 Buratal
  • γ-(2,4-Dichlorophenoxy)butyric acid
  • Γ-(2,4-DICHLORPHENOXY)-BUTTERSAEURE
  • BRN 1976809
  • Butirex
  • Butormone
  • Butoxon
  • Butoxone
  • Butoxone amine
  • Butoxone ester
  • Butyrac 200
  • Butyrac ester
  • Caswell No. 316
  • gamma-(2,4-Dichlorophenoxy)butyric acid
  • EINECS 202-366-9
  • EPA Pesticide Chemical Code 030801
  • Kyselina 4-(2,4-dichlorfenoxy)maselna
  • UNII-SL314DW868
  • 4-(2,4-DB)
  • gamma-(2,4-dichlorophenoxy)-butanoic acid
  • gamma-(2,4-dichlorophenoxy)-butyric acid
  • gamma-(2,4-dichlorophenoxy)butanoic acid
  • gamma-(2,4-dichlorophenoxy)butyric acid

Applications:

Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns

September 9, 2015

4-(2,4-dichlorophenoxy)butyric acid or 2,4-DB is a herbicides used in alfalfa, peanuts, and soybean production. 2,4-DB selectively controls broadleaf weeds inhibiting growth at the root and stem tips. It is considered slightly toxic, as is its metabolite 2,4-D. The EURL (European Union Reference Laboratory) included 2,4-DB in an analysis of acidic pesticides using QuEChERS methodology. Primesep 100 and Obelisc R were used to retain and separate 2,4-DB from impurities. Method is LC/MS compatible and developed to be used for many pesticides.

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4-DB

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
2,4-DB

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.