2,5-Dihydroxybenzoic Acid

CAS Number 490-79-9
Molecular Formula C7H6O4
Molecular Weight 154.122 g/mol
LogP 1.74
  • Benzoic acid, 2,5-dihydroxy-
  • 2,5-Dihydroxybenzoic acid
  • 490-79-9
  • 4-10-00-01441
  • 2,5-Dihydroxybenzoic acid
  • 2,5-Dioxybenzoic acid
  • 3,6-Dihydroxybenzoic acid
  • 5-Hydroxysalicylic acid
  • Acide gentisique
  • acido gentisico
  • Carboxyhydroquinone
  • Gensigen
  • Gensigon
  • gentisic acid
  • Gentisinic acid
  • Gentisinsaure
  • Hydroquinonecarboxylic acid
  • NSC 27224
  • NSC 49098
  • NSC 78825
  • BRN 2209119
  • EINECS 207-718-5
  • Gentisate
  • Salicylic acid, 5-hydroxy-
  • Acido 2,5-diidrossibenzoico
  • Acidum gentisicum
  • Kyselina 2,5-dihydroxybenzoova
  • Kyselina gentisinova
  • UNII-VP36V95O3T
  • 2,5-Dioxybenzoate
  • 3,6-Dihydroxybenzoate
  • 5-Hydroxy-Salicylate
  • 5-Hydroxy-Salicylic acid
  • 5-Hydroxysalicylate
  • Dihydroxybenzoicacid
  • Gentisinate
  • Hydroquinonecarboxylate


Mixed-Mode Separation of Dihydroxybenzoic Acids

Primesep B offers better selectivity over traditional, reversed-phase C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, 2,5-dihydroxybenzoic 3,4-dihydroxybenzoic, 3,5-dihydroxybenzoic acids) Primesep B combines reversed-phase and anion-exchange mechanism with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid
Carboxylic Acids

HPLC Separation of Dihydroxybenzoic Acid

Separation of hydrophilic isomers is very difficult when only one mechanism of retention, like reversed-phase, is available. Isomers have very similar hydrophobic properties. In case of mixed-mode chromatography, small difference in hydrophobic and ionic properties allows to separate isomers. Isomers of dihydroxybenzoic acids are separated on a Primesep D column with good selectivity and peak shape. This method can be used for separation of other hydrophilic acidic compounds as well as hydrophobic basic compound.

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid

HPLC separation of Dihydroxybenzoic acid in Hydrogen-Bonding mode on SHARC 1 HPLC column



Application Notes: Dihydroxybenzoic acids are polar acidic compounds containing two hydroxyl groups as well as a carboxylic acid. Isomers of dihydroxybenzoic acids are very polar with varying degrees of acidity. While the isomers can be analyzed by mixed-mode chromatography on Primesep D column, the presence of hydroxyls and carboxylic acid fragments also make these compounds good candidates for using the new hydrogen bonding columns for analysis. Since all these compounds have the same amount of hydroxyls they are separated based on accessibility of this hydroxyl group. Retention time can be adjusted by the amount of ACN, MeOh and concentration of additives (formic acid, ammonium formate, triethylamine, etc.). Our method is fully compatible with LC/MS and prep chromatography and can be used for other hydroxy and carboxylic acids containing compounds..

Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A. To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: 2,6-dihydroxybenzoic acid, 2,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid

Detection Technique: UV, LC/MS

Application Analytes:

2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid