|Molecular Weight||94.117 g/mol|
Three isomers of aminopyridine separate well by reversed-phase cation-exchange mixed-mode chromatography on an Obelisc R HPLC column. The presence of additional mechanism of interaction provides great selectivity for separation of closely related compounds. Elution of compounds can be monitored by UV, Evaporative Light-Scattering Detector (ELSD), Corona (CAD) or LC/MS. This method was validated at a pharmaceutical company. This HPLC method can be adopted as general approach for analysis of aminopyridine and other isomeric compounds.
Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are sensitive to variations of mobile phase. The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers.
Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.
Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine
Detection Technique: UV, LC/MS