2-Aminopyridine

CAS Number 504-29-0
Molecular Formula C5H6N2
Molecular Weight 94.117 g/mol
InChI Key ICSNLGPSRYBMBD-UHFFFAOYSA-N
LogP 0.48
Synonyms
  • 2-Aminopyridine
  • Pyridin-2-amine
  • 2-Pyridinamine
  • 504-29-0
  • 2-Pyridinamine
  • 1,2-Dihydro-2-iminopyridine
  • 2-AMINOPYRIDIN
  • 2-piridilamina
  • 2-Pyridinylamine
  • 2-Pyridylamin
  • 2-Pyridylamine
  • NSC 431
  • o-Aminopyridine
  • Pyridin-2-ylamine
  • PYRIDINE, 2-AMINO-
  • α-Aminopyridine
  • α-Pyridinamine
  • α-Pyridylamine
  • Amino-2 pyridine
  • 2-Aminopryidine
  • EINECS 207-988-4
  • alpha-Pyridinamine
  • alpha-Pyridylamine
  • UNII-WSX981HEWU
  • 102769-74-4
  • 29212-31-5
  • 45505-67-7

Applications:


Comparison of Obelisc R to C18 columns for the Separation of Aminopyridine Isomers


Three isomers of aminopyridine separate well by reversed-phase cation-exchange mixed-mode chromatography on an Obelisc R HPLC column. The presence of additional mechanism of interaction provides great selectivity for separation of closely related compounds. Elution of compounds can be monitored by UV, Evaporative Light-Scattering Detector (ELSD), Corona (CAD) or LC/MS. This method was validated at a pharmaceutical company. This HPLC method can be adopted as general approach for analysis of aminopyridine and other isomeric compounds.



Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column
  Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are  sensitive to variations of mobile phase.  The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with  LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers. Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site. Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine Detection Technique: UV, LC/MS  

Condition

Column Sharc 1, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN/MeOH
Buffer Fa, AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine
 

Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine

HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase
 

Condition

Column Sharc 1, 4.6x100 mm, 5 µm, 100A
Mobile Phase MeCN - 97.5%
Buffer H3PO4 - 2.5%
Flow Rate 3.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds Drug, Muscle strengthener, Hydrophilic, Ionizable, Vertebrate pesticide
Analyzing Compounds 2-Aminopyridine,  3-Aminopyridine, 4-Aminopyridine, Pyridine
 

Application Analytes:

2-Aminopyridine
3-Aminopyridine
4-Aminopyridine
Pyridine