2-Morpholinoethanesulfonic Acid (MES)

2-Morpholinoethanesulfonic Acid (MES) structural formula

CAS Number4432-31-9
Molecular FormulaC6H13NO4S
Molecular Weight195.230
InChI KeySXGZJKUKBWWHRA-UHFFFAOYSA-N
LogP-2.12
Synonyms
  • 4-Morpholineethanesulfonic acid
  • 2-(Morpholin-4-yl)ethane-1-sulfonic acid
  • 4432-31-9
  • BRN 0141319
  • EINECS 224-632-3
  • Ethanesulfonic acid, 2-morpholino-
  • Morpholineethanesulfonic acid
  • Morpholinoethanesulfonic acid
  • Morpholino-N-ethylsulfonate
  • NSC 157116
  • 2-(4-Morpholinyl)ethanesulfonic acid
  • UNII-2GNK67Q0C4
  • 2-(N-Morpholino)aethansulfonsaeure
  • 2-(N-Morpholino)ethansulfonsaeure
  • 2-(N-morpholino)ethanesulfonic acid
  • 2-(morpholin-4-yl)ethanesulfonic acid
  • 2-morpholin-4-ylethanesulfonic acid
  • 2-morpholinoethanesulfonic acid
  • 2-morpholinoethanesulphonic acid
  • 4-morpholinethanesulfonic acid
  • MES

Applications:

HPLC Separation of Morpholino Sulfates

November 21, 2010


MOPS and MES are buffers used in biology and biochemistry. They consist of a morpholine ring and propanesulfonic acid. Both molecules are zwitter-ionic and very polar. No retention can be achieved on reversed-phase columns. MOPS and MES are analyzed in HILIC mode on a Primesep N HPLC column. Other biological buffers can be analyzed with this general method. Biological buffers are not UV active and can be monitored by ELSD, CAD or LC/MS.

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)
Zwitterion

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Effect of Buffer on HPLC Separation of Buffers

August 22, 2008

HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)
CAPS (3-(Cyclohexylamino)propanesulfonic acid)
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)
Zwitterion

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.