2,4,5-Trichlorophenoxyacetic acid

2,4,5-Trichlorophenoxyacetic acid structural formula

CAS Number 93-76-5
Molecular Formula C8H5Cl3O3
Molecular Weight 255.480 g/mol
InChI Key SMYMJHWAQXWPDB-UHFFFAOYSA-N
LogP 3.31
Synonyms
  • 2,4,5-Trichlorophenoxyacetic acid
  • (2,4,5-Trichlorophenoxy)acetic acid
  • Acetic acid, 2-(2,4,5-trichlorophenoxy)-
  • 93-76-5
  • 2,4,5-T
  • 4-06-00-00973
  • Acetic acid, (2,4,5-trichlorophenoxy)-
  • (2,4,5-Trichlorophenoxy)acetic acid
  • 2,4,5-Trichlorophenoxyacetate
  • Acetic acid, 2-(2,4,5-trichlorophenoxy)-
  • Arbokan
  • BCF-Bushkiller
  • NSC 430
  • Trichlorophenoxyacetic acid
  • Trioxon
  • BRN 2055620
  • Brush Killer
  • Brush RHAP
  • Brush-off 445 low volatile brush killer
  • Brushtox
  • Caswell No. 881
  • Crossbow
  • Decamine 4T
  • Ded-Weed
  • Ded-weed brush killer
  • Ded-weed lv-6 brush kil and t-5 brush kil
  • EINECS 202-273-3
  • Envert-T
  • EPA Pesticide Chemical Code 082001
  • Estercide t-2 and t-245
  • Farmco fence rider
  • Fence rider
  • Fruitone A
  • Inverton 245
  • Line rider
  • Phortox
  • RCRA waste number U232
  • Spontox
  • Super D Weedone
  • Tippontormona
  • Visko Rhap
  • Visko rhap low volatile ester
  • (2,4,5-Trichloor-fenoxy)-azijnzuur
  • (2,4,5-Trichlor-phenoxy)-essigsaeure
  • Acide 2,4,5-trichloro phenoxyacetique
  • Acido (2,4,5-tricloro-fenossi)-acetico
  • Cido (2,4,5-tricloro-fenossi)-acetico
  • Kwas 2,4,5-trojchlorofenoksyoctowy
  • UNII-9Q963S4YMX
  • (2,4,5-Trichlorphenoxy)essigsaeure
  • 2,4,5-Trichlorphenoxyessigsaeure
  • 2-(2,4,5-trichlorophenoxy)acetic acid
  • 2-(2,4,5-trichlorophenoxy)ethanoic acid
  • Esteron 245
  • Trioxone

Applications:


Analysis of 2,4,5-Trichlorophenoxyacetic acid on Primesep and Obelisc HPLC Columns
   
2,4,5-Trichlorophenoxyacetic acid (2,4,5-T) is a synthetic auxin and herbicide that was widely used until the 1970s. The EURL (European Union Reference Laboratory) included a QuEChERS (Quick, Easy, Cheap, Rugged, and Safe) method for 2,4,5-T as a target pesticide for EUPT-SRM. Obelisc R and Primesep B2 were used to retain 2,4,5-T and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. This method is LC/MS compatible and can be used as a general approach for analyzing 2,4,5-T and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4.5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-Trichlorophenoxyacetic acid
 

Application Analytes:

2,4,5-Trichlorophenoxyacetic acid

HPLC Analysis of 2,4,5-T on Mixed-Mode Columns
  2,4,5-T is a synthetic herbicide that defoliates broad-leaf plants. It was a major component of Agent Orange, a defoliant used during the Vietnam War. 2,4,5-T has been replaced by dicamba and triclopyr due to its possible toxicity. 2,4,5-T was included in a proficiency test ran by the EURL (European Union Reference Laboratory) using QuEChERS methodology on acidic pesticides. Obelisc R retained 2,4,5-T because it has a long hydrophobic chain and ionic pairing groups. Primesep 100 retains by reverse-phase and with acid ion-pairing groups. Method is LC/MS compatible and same conditions can be used on many different pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 2,4,5-T
 

Application Analytes:

2,4,5-Trichlorophenoxyacetic acid

HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column
Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.

Condition

Column Newcrom BH, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN, MeOH
Buffer H2SO4, Formic acid, AmFm pH 3.0
Flow Rate 0.6 ml/min
Detection UV 275 nm

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Monocrotophos, Metribuzin, Bromacil, Carbofuran, 4-Chlorophenoxyacetic acid, Bentazone, Folpet, Fenthion, Fenvalerate, Metrabuzin, Carbofuran, Atrazine, Parathion –methyl, MCPA, Clopyralid, Dicamba, Benthazon, Simazine, Diuron, MCPB, Mecoprop, 4-Chlorophenoxyacetic acid,2,4,5-T


Application Analytes:

2,4,5-Trichlorophenoxyacetic acid
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Monocrotophos
Simazine