3,4-Dihydroxybenzoic Acid

CAS Number 99-50-3
Molecular Formula C7H6O4
Molecular Weight 154.122 g/mol
InChI Key YQUVCSBJEUQKSH-UHFFFAOYSA-N
LogP 0.86
Synonyms
  • 3,4-Dihydroxybenzoic acid
  • Benzoic acid, 3,4-dihydroxy-
  • 99-50-3
  • Protocatechuic acid
  • Benzoic acid, 3,4-dihydroxy-
  • 1,2-Dihydroxybenzene-4-carboxylic acid
  • 3,4-Dihydroxybenzoesaure
  • 4,5-Dihydroxybenzoic acid
  • 4-Carboxy-1,2-dihydroxybenzene
  • acide 3,4-dihydroxybenzoique
  • acido 3,4-dihidroxibenzoico
  • NSC 16631
  • Protocatechoic acid
  • BRN 1448841
  • EINECS 202-760-0
  • UNII-36R5QJ8L4B
  • 2,4-Dihydroxybenzoate
  • 2,4-Dihydroxybenzoic acid
  • 3,4-Dihydroxybenzoate
  • Protocatehuic acid
  • b-Resorcylate
  • b-Resorcylic acid
  • beta-Resorcylic acid
  • beta-resorcylate

Applications:


Mixed-Mode Separation of Dihydroxybenzoic Acids



Primesep B offers better selectivity over traditional, reversed-phase C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, 2,5-dihydroxybenzoic 3,4-dihydroxybenzoic, 3,5-dihydroxybenzoic acids) Primesep B combines reversed-phase and anion-exchange mechanism with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid
Carboxylic Acids

HPLC Separation of Dihydroxybenzoic Acid


Separation of hydrophilic isomers is very difficult when only one mechanism of retention, like reversed-phase, is available. Isomers have very similar hydrophobic properties. In case of mixed-mode chromatography, small difference in hydrophobic and ionic properties allows to separate isomers. Isomers of dihydroxybenzoic acids are separated on a Primesep D column with good selectivity and peak shape. This method can be used for separation of other hydrophilic acidic compounds as well as hydrophobic basic compound.



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid