3,4-Dihydroxybenzoic Acid

CAS Number 99-50-3
Molecular Formula C7H6O4
Molecular Weight 154.122 g/mol
InChI Key YQUVCSBJEUQKSH-UHFFFAOYSA-N
LogP 0.86
Synonyms
  • 3,4-Dihydroxybenzoic acid
  • Benzoic acid, 3,4-dihydroxy-
  • 99-50-3
  • Protocatechuic acid
  • Benzoic acid, 3,4-dihydroxy-
  • 1,2-Dihydroxybenzene-4-carboxylic acid
  • 3,4-Dihydroxybenzoesaure
  • 4,5-Dihydroxybenzoic acid
  • 4-Carboxy-1,2-dihydroxybenzene
  • acide 3,4-dihydroxybenzoique
  • acido 3,4-dihidroxibenzoico
  • NSC 16631
  • Protocatechoic acid
  • BRN 1448841
  • EINECS 202-760-0
  • UNII-36R5QJ8L4B
  • 2,4-Dihydroxybenzoate
  • 2,4-Dihydroxybenzoic acid
  • 3,4-Dihydroxybenzoate
  • Protocatehuic acid
  • b-Resorcylate
  • b-Resorcylic acid
  • beta-Resorcylic acid
  • beta-resorcylate

Applications:


Mixed-Mode Separation of Dihydroxybenzoic Acids
Primesep B offers better selectivity over traditional, reversed-phase C18 columns for separating regioisomers of aromatic dihydroxybenzoic acids (2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, 2,5-dihydroxybenzoic 3,4-dihydroxybenzoic, 3,5-dihydroxybenzoic acids) Primesep B combines reversed-phase and anion-exchange mechanism with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).

Condition

Column Primesep B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds 3.4-dihydroxybenzoic acid, 3.5-dihydroxybenzoic acid,2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid
Carboxylic Acids

HPLC Separation of Dihydroxybenzoic Acid
Separation of hydrophilic isomers is very difficult when only one mechanism of retention, like reversed-phase, is available. Isomers have very similar hydrophobic properties. In case of mixed-mode chromatography, small difference in hydrophobic and ionic properties allows to separate isomers. Isomers of dihydroxybenzoic acids are separated on a Primesep D column with good selectivity and peak shape. This method can be used for separation of other hydrophilic acidic compounds as well as hydrophobic basic compound.

Condition

Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds 3.4-dihydroxybenzoic acid, 3.5-dihydroxybenzoic acid,2,4-dihydroxybenzoic acid, 2,5-dihydroxybenzoic acid, 2,3-dihydroxybenzoic acid
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,4-Dihydroxybenzoic Acid
2,5-Dihydroxybenzoic Acid
3,4-Dihydroxybenzoic Acid
3,5-Dihydroxybenzoic Acid