3-Aminobenzoic Acid

3-Aminobenzoic Acid structural formula

CAS Number99-05-8
Molecular FormulaC7H7NO2
Molecular Weight137.139
InChI KeyXFDUHJPVQKIXHO-UHFFFAOYSA-N
LogP0.65
Synonyms
  • Benzoic acid, 3-amino-
  • 3-Aminobenzoic acid
  • 99-05-8
  • 4-14-00-01092
  • 3-Aminobenzenecarboxylic acid
  • 3-AMINOBENZOESAEURE
  • 3-Aminobenzoesaure
  • 3-aminobenzoic acid
  • 3-Carboxyaniline
  • Acide 3-aminobenzoique
  • acido 3-aminobenzoico
  • Aniline-3-carboxylic acid
  • BENZOIC ACID, M-AMINO-
  • m-Aminobenzoic acid
  • m-Anthranilic acid
  • m-Carboxyaniline
  • m-Carboxyphenylamine
  • NSC 15012
  • BRN 0471603
  • EINECS 202-724-4
  • UNII-G2X3B3O37U
  • 3-Aminobenzoate
  • Aniline-3-carboxylate
  • M-Amonibenzoate
  • M-Amonibenzoic acid
  • m-Aminobenzoesaeure
  • 1190921-66-4
  • 915120-54-6

Applications:

Separation of Isomers of Aminobenzoic Acid

October 15, 2015

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Acid, Hydrophobic, Ionizable
Analyzing Compounds 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Isomers of Aminobenzoic Acid

March 27, 2011

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Acid, Hydrophobic, Ionizable
Analyzing Compounds 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid

 

 

 

 

 

 

Aminobenzoic Acid is an organic acid that can be found in folic acid vitamins, grains, eggs, milk, and meat. Because it absorbs the UV light, it is used in sunscreen with SPF 15 or greater. The acid can cause damage to DNA and skin irritations in rare cases. Primesep 100, a reverse phase column, contains embedded acidic ionizable groups and can retain aminobenzoic acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Isomers of organic compounds usually have very similar properties. Separation of such isomers by HPLC requires intensive screening of various columns and mobile phase conditions. In case of hydrophilic compounds, this task becomes even more challenging due to the lack of or limited retention on traditional reversed-phase (C18 or C8) HPLC columns. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were successfully separated on a Primesep 100 reversed-phase/cation-exchange column. Baseline separation within 10 minutes was achieved and produced a perfect peak shapes for all three analytes. The method can be used a generic approach for separation of ionic hydrophobic and ionic basic isomers by mixed-mode chromatography.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Amino Acids on Obelisc R Column

October 4, 2007


Closely related compounds like amino acids can be separated on an Obelisc R column by various buffers depending on the amount of baseline separation required. By choosing different buffers, the separation between compounds can be adjusted based on application needs, especially those that require low organic concentration in the mobile phase. UV detection at 250nm.

Condition

Column Obelisc R, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
3-Aminobenzoic Acid
Aspartame
Phenylalanine
Tryptophan

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.