CAS Number 462-08-8
Molecular Formula C5H6N2
Molecular Weight 94.117 g/mol
LogP 0.11
  • 3-Pyridinamine
  • Pyridin-3-amine
  • 462-08-8
  • 5-22-09-00003
  • 3-Aminopyridine
  • 3-Amino-Pyridine
  • 3-piridilamina
  • 3-Pyridinylamine
  • 3-Pyridylamin
  • 3-Pyridylamine
  • m-Aminopyridine
  • NSC 15040
  • β-Aminopyridine
  • Amino-3 pyridine
  • BRN 0105692
  • EINECS 207-322-2
  • UNII-69JE8P2L84


Comparison of Obelisc R to C18 columns for the Separation of Aminopyridine Isomers

Three isomers of aminopyridine separate well by reversed-phase cation-exchange mixed-mode chromatography on an Obelisc R HPLC column. The presence of additional mechanism of interaction provides great selectivity for separation of closely related compounds. Elution of compounds can be monitored by UV, Evaporative Light-Scattering Detector (ELSD), Corona (CAD) or LC/MS. This method was validated at a pharmaceutical company. This HPLC method can be adopted as general approach for analysis of aminopyridine and other isomeric compounds.

Application Analytes:


HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities

Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties.

Application Analytes:

Ascorbic Acid
Barbituric Acid
Isonicotinic Acid
Nicotinic Acid
Vitamin B2 (Riboflavin)
Vitamin B6 (Pyridoxine)

HPLC Separation of Aminopyridines Isomers in Hydrogen-Bonding mode on a SHARC 1 HPLC Column


Application Notes: Pyridines and aminopyridines are hydrophilic basic compounds. Traditionally these compounds have been separated and analyzed by GC and HPLC. In the case of HPLC, reversed-phase chromatography with ion-pairing reagent is used along with alternative modes like HILIC for separation. Mixed-mode chromatography can also be used to successfully separate isomers of substituted pyridines. However, we developed a new mode of separation for these compounds with hydrogen bonding. Isomers of aminopyridine are separated based on hydrogen bonding interaction between analyte and stationary phase. Mobile phase utilizes combination of acetonitrile and methanol with additives. Retention time and selectivity are  sensitive to variations of mobile phase.  The order of elution changes depending on the amount of acetonitrile, methanol, formic acid and ammonium formate. This method and approach is compatible with  LC/MS and prep chromatography and can be used for separation of other pyridine based compounds and pyridine based isomers.

Application Columns: SHARC 1, 3.2x100 mm, 5 um, 100A, To learn more about SHARC 1 columns click here. To order this column click here. To see more chromatographic separations check our web site.

Application Compounds: pyridine, 2-aminopyridine, 3-aminopyridine, 4-aminopyridine

Detection Technique: UV, LC/MS 




Application Analytes:


HPLC Separation of Aminopyridines in Non-Aqueous Mobile Phase

Application Analytes: