4-Aminobenzoic Acid

Applications:

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HPLC Separation of Isomers of Aminobenzoic Acid

Condition

Column	Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN
Buffer	H3PO4, H2SO4
Flow Rate	1.0 ml/min
Detection	UV, 230 nm

 

Description

Class of Compounds	Acid, Hydrophobic, Ionizable
Analyzing Compounds	4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid

            Aminobenzoic Acid is an organic acid that can be found in folic acid vitamins, grains, eggs, milk, and meat. Because it absorbs the UV light, it is used in sunscreen with SPF 15 or greater. The acid can cause damage to DNA and skin irritations in rare cases. Primesep 100, a reverse phase column, contains embedded acidic ionizable groups and can retain aminobenzoic acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Isomers of organic compounds usually have very similar properties. Separation of such isomers by HPLC requires intensive screening of various columns and mobile phase conditions. In case of hydrophilic compounds, this task becomes even more challenging due to the lack of or limited retention on traditional reversed-phase (C18 or C8) HPLC columns. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were successfully separated on a Primesep 100 reversed-phase/cation-exchange column. Baseline separation within 10 minutes was achieved and produced a perfect peak shapes for all three analytes. The method can be used a generic approach for separation of ionic hydrophobic and ionic basic isomers by mixed-mode chromatography.

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2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid

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USP Methods Analysis of Procainamide Legacy L1 Column

  Application Columns: Legacy L1 C18 HPLC column Application compounds: Procainamide and 4-aminobenzoic acid Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid Detection technique: UV Reference: USP35: NF30

Condition

Column	Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase	Water/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
Buffer	TEAPh
Flow Rate	1.5 ml/min
Detection	UV, 230 nm

 

Description

Class of Compounds	Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing Compounds	Ibuprofen, benzophenone

 

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4-Aminobenzoic Acid
Procainamide
Procainamide Hydrochloride

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USP Methods for the Analysis of Procainamide

  Application Notes: Procainamide is a common antiarrhythmic agent used to treat cardiac arrhythmias. According to USP methods procainamide hydrochloride contains not less than 98% and no more than 102% procainamide based on the dried basis. The USP HPLC method for the separation of procainamide and 4-aminobenzoic was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Reference: USP 35- NF30

Condition

Column	Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeOH/H2O - 60/40%
Buffer	TEAPh pH 7.5
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

 

Description

Class of Compounds	Drug, Antiarrhythmic, Hydrophobic, Ionizable
Analyzing Compounds	Procainamide, 4-Aminobenzoic acid

 

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4-Aminobenzoic Acid
Procainamide

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Separation of Isomers of Aminobenzoic Acid

Condition

Column	Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN
Buffer	H3PO4, H2SO4
Flow Rate	1.0 ml/min
Detection	UV, 230 nm

 

Description

Class of Compounds	Acid, Hydrophobic, Ionizable
Analyzing Compounds	4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid

 

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2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid

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