4-Aminobenzoic Acid

4-Aminobenzoic Acid structural formula

CAS Number 150-13-0
Molecular Formula C7H7NO2
Molecular Weight 137.139 g/mol
InChI Key ALYNCZNDIQEVRV-UHFFFAOYSA-N
LogP 0.83
Synonyms
  • 4-Aminobenzoic acid
  • Benzoic acid, 4-amino-
  • 150-13-0
  • Benzoic acid, 4-amino-
  • 4-Aminobenzenecarboxylic acid
  • 4-AMINOBENZOESAEURE
  • 4-Aminobenzoesaure
  • 4-Carboxyaniline
  • 4-Carboxyphenylamine
  • Acide 4-aminobenzoique
  • acido 4-aminobenzoico
  • Actipol
  • Aniline-4-carboxylic acid
  • Anticanitic vitamin
  • Anticantic vitamin
  • Antichromotrichia factor
  • Anti-Chromotrichia factor
  • Bacterial vitamin H1
  • Benzoic acid, p-amino-
  • Chromotrichia factor
  • Hachemina
  • NSC 7627
  • Pabacyd
  • Pabafilm
  • Pabamine
  • P-AMINOBENZOIC ACID
  • Paraminol
  • Paranate
  • p-Carboxyaniline
  • p-Carboxyphenylamine
  • Romavit
  • Sunbrella
  • Trichochromogenic factor
  • Vitamin BX
  • Vitamin H'
  • 1-Amino-4-carboxybenzene
  • BRN 0471605
  • Caswell No. 033B
  • EINECS 205-753-0
  • EPA Pesticide Chemical Code 233300
  • Super Shade by Coppertone
  • UNII-TL2TJE8QTX
  • 4-Aminobenzoate
  • Acido p-aminobenzoico
  • Acidum paraminobenzoicum
  • Amben
  • Aminobenzoate
  • Aminobenzoic acid
  • Aniline-4-carboxylate
  • Kyselina p-aminobenzoova
  • PAB
  • PABA
  • Pabagel
  • Pabanol
  • Papacidum
  • Para-aminobenzoate
  • Potaba
  • RVPaba Lipstick
  • Rvpaba
  • Super Shade by Coppertone
  • Trochromogenic factor
  • gamma-Aminobenzoate
  • gamma-Aminobenzoic acid
  • p-Amino-Benzoate
  • p-Amino-Benzoic acid
  • p-Aminobenzoesaeure
  • para-aminobenzoic acid
  • 8014-65-1

Applications:

HPLC Separation of Isomers of Aminobenzoic Acid
 
Aminobenzoic Acid is an organic acid that can be found in folic acid vitamins, grains, eggs, milk, and meat. Because it absorbs the UV light, it is used in sunscreen with SPF 15 or greater. The acid can cause damage to DNA and skin irritations in rare cases. Primesep 100, a reverse phase column, contains embedded acidic ionizable groups and can retain aminobenzoic acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Isomers of organic compounds usually have very similar properties. Separation of such isomers by HPLC requires intensive screening of various columns and mobile phase conditions. In case of hydrophilic compounds, this task becomes even more challenging due to the lack of or limited retention on traditional reversed-phase (C18 or C8) HPLC columns. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were successfully separated on a Primesep 100 reversed-phase/cation-exchange column. Baseline separation within 10 minutes was achieved and produced a perfect peak shapes for all three analytes. The method can be used a generic approach for separation of ionic hydrophobic and ionic basic isomers by mixed-mode chromatography.

Application Analytes:

2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid
USP Methods Analysis of Procainamide Legacy L1 Column
  Application Columns: Legacy L1 C18 HPLC column Application compounds: Procainamide and 4-aminobenzoic acid Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid Detection technique: UV Reference: USP35: NF30

Condition
Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Water/MeOH/TEA (140/60/1)   adjusted to pH 7.5 with H3PO4
Buffer TEAPh
Flow Rate 1.5 ml/min
Detection UV, 230 nm
 

Description
Class of Compounds Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable
Analyzing Compounds Ibuprofen, benzophenone
 
Application Analytes:

4-Aminobenzoic Acid
Procainamide
Procainamide Hydrochloride
USP Methods for the Analysis of Procainamide
  Application Notes: Procainamide is a common antiarrhythmic agent used to treat cardiac arrhythmias. According to USP methods procainamide hydrochloride contains not less than 98% and no more than 102% procainamide based on the dried basis. The USP HPLC method for the separation of procainamide and 4-aminobenzoic was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS. Reference: USP 35- NF30

Condition
Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O - 60/40%
Buffer TEAPh pH 7.5
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description
Class of Compounds Drug, Antiarrhythmic, Hydrophobic, Ionizable
Analyzing Compounds Procainamide, 4-Aminobenzoic acid
 
Application Analytes:

4-Aminobenzoic Acid
Procainamide
Separation of Isomers of Aminobenzoic Acid

Condition
Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 230 nm
 

Description
Class of Compounds Acid, Hydrophobic, Ionizable
Analyzing Compounds 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid
 
Application Analytes:

2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid