4-Aminobenzoic Acid

CAS Number	150-13-0
Molecular Formula	C7H7NO2
Molecular Weight	137.139 g/mol
InChI Key	ALYNCZNDIQEVRV-UHFFFAOYSA-N
LogP	0.83
Synonyms	4-Aminobenzoic acid Benzoic acid, 4-amino- 150-13-0 Benzoic acid, 4-amino- 4-Aminobenzenecarboxylic acid 4-AMINOBENZOESAEURE 4-Aminobenzoesaure 4-Carboxyaniline 4-Carboxyphenylamine Acide 4-aminobenzoique acido 4-aminobenzoico Actipol Aniline-4-carboxylic acid Anticanitic vitamin Anticantic vitamin Antichromotrichia factor Anti-Chromotrichia factor Bacterial vitamin H1 Benzoic acid, p-amino- Chromotrichia factor Hachemina NSC 7627 Pabacyd Pabafilm Pabamine P-AMINOBENZOIC ACID Paraminol Paranate p-Carboxyaniline p-Carboxyphenylamine Romavit Sunbrella Trichochromogenic factor Vitamin BX Vitamin H' 1-Amino-4-carboxybenzene BRN 0471605 Caswell No. 033B EINECS 205-753-0 EPA Pesticide Chemical Code 233300 Super Shade by Coppertone UNII-TL2TJE8QTX 4-Aminobenzoate Acido p-aminobenzoico Acidum paraminobenzoicum Amben Aminobenzoate Aminobenzoic acid Aniline-4-carboxylate Kyselina p-aminobenzoova PAB PABA Pabagel Pabanol Papacidum Para-aminobenzoate Potaba RVPaba Lipstick Rvpaba Super Shade by Coppertone Trochromogenic factor gamma-Aminobenzoate gamma-Aminobenzoic acid p-Amino-Benzoate p-Amino-Benzoic acid p-Aminobenzoesaeure para-aminobenzoic acid 8014-65-1

Applications:

HPLC Separation of Isomers of Aminobenzoic Acid

Aminobenzoic Acid is an organic acid that can be found in folic acid vitamins, grains, eggs, milk, and meat. Because it absorbs the UV light, it is used in sunscreen with SPF 15 or greater. The acid can cause damage to DNA and skin irritations in rare cases. Primesep 100, a reverse phase column, contains embedded acidic ionizable groups and can retain aminobenzoic acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Isomers of organic compounds usually have very similar properties. Separation of such isomers by HPLC requires intensive screening of various columns and mobile phase conditions. In case of hydrophilic compounds, this task becomes even more challenging due to the lack of or limited retention on traditional reversed-phase (C18 or C8) HPLC columns. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were successfully separated on a Primesep 100 reversed-phase/cation-exchange column. Baseline separation within 10 minutes was achieved and produced a perfect peak shapes for all three analytes. The method can be used a generic approach for separation of ionic hydrophobic and ionic basic isomers by mixed-mode chromatography.

2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid

USP Methods Analysis of Procainamide Legacy L1 Column

Application Columns: Legacy L1 C18 HPLC column Application compounds: Procainamide and 4-aminobenzoic acid Mobile phase: WAter/MeOH/MeCN (70/70/90) with 7 mM sodium lauryl sulfate and 11mM phosphoric acid Detection technique: UV Reference: USP35: NF30

4-Aminobenzoic Acid

USP Methods for the Analysis of Procainamide

Application Notes: Procainamide is a common antiarrhythmic agent used to treat cardiac arrhythmias. According to USP methods procainamide hydrochloride contains not less than 98% and no more than 102% procainamide based on the dried basis. The USP HPLC method for the separation of procainamide and 4-aminobenzoic was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.

Reference: USP 35- NF30

4-Aminobenzoic Acid
Procainamide

Separation of Isomers of Aminobenzoic Acid

2-Aminobenzoic Acid
3-Aminobenzoic Acid
4-Aminobenzoic Acid