CAS Number | 150-13-0 |
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Molecular Formula | C7H7NO2 |
Molecular Weight | 137.139 g/mol |
InChI Key | ALYNCZNDIQEVRV-UHFFFAOYSA-N |
LogP | 0.83 |
Synonyms |
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Column | Primesep 100, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | H3PO4, H2SO4 |
Flow Rate | 1.0 ml/min |
Detection | UV, 230 nm |
Class of Compounds | Acid, Hydrophobic, Ionizable |
Analyzing Compounds | 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid |
Isomers of organic compounds usually have very similar properties. Separation of such isomers by HPLC requires intensive screening of various columns and mobile phase conditions. In case of hydrophilic compounds, this task becomes even more challenging due to the lack of or limited retention on traditional reversed-phase (C18 or C8) HPLC columns. Three isomers of aminobenzoic acid (2-aminobenzoic acid, 3-aminobenzoic acid and 4-aminobenzoic acid) were successfully separated on a Primesep 100 reversed-phase/cation-exchange column. Baseline separation within 10 minutes was achieved and produced a perfect peak shapes for all three analytes. The method can be used a generic approach for separation of ionic hydrophobic and ionic basic isomers by mixed-mode chromatography.
Column | Legacy L1, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | Water/MeOH/TEA (140/60/1) adjusted to pH 7.5 with H3PO4 |
Buffer | TEAPh |
Flow Rate | 1.5 ml/min |
Detection | UV, 230 nm |
Class of Compounds | Drug, Nonsteroidal anti-inflammatory drug, Hydrophobic, Ionizable |
Analyzing Compounds | Ibuprofen, benzophenone |
Column | Legacy L1, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeOH/H2O - 60/40% |
Buffer | TEAPh pH 7.5 |
Flow Rate | 1.0 ml/min |
Detection | UV, 270 nm |
Class of Compounds | Drug, Antiarrhythmic, Hydrophobic, Ionizable |
Analyzing Compounds | Procainamide, 4-Aminobenzoic acid |
Column | Primesep 100, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN |
Buffer | H3PO4, H2SO4 |
Flow Rate | 1.0 ml/min |
Detection | UV, 230 nm |
Class of Compounds | Acid, Hydrophobic, Ionizable |
Analyzing Compounds | 4-Aminobenzoic acid, 3-Aminobenzoic acid, 2-Aminobenzoic acid |
Column | Sharc 1, 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN/MeOH |
Buffer | Formic Acid 0.1% , AmFm - 0.01% |
Flow Rate | 1.0 ml/min |
Detection | UV 270 nm |
Class of Compounds | Nucleoside monophosphate, Hydrophilic, Ionizable |
Analyzing Compounds | Inosine, Acedoben |