|Molecular Weight||169.228 g/mol|
Aminobiphenyls consist of isomers that differ only in the location of a primary amine group on an aromatic ring. 2-, 3-, and 4-aminobiphenyl are separated on a Primesep D with good peak shape and a short retention time by a mixture of reversed-phase and ion-exclusion interactions. Peak order can be reversed for 2- and 3-aminobiphenyls by using ammonium formate buffer without pH adjustment. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate buffer or acetic acid (HOAc) with UV detection at 250 nm.
|Column||Primesep S2, 4.6x150 mm, 5 µm, 100A|
|Mobile Phase||MeCN/H2O - 5/95%|
|Buffer||AmAc pH 5.0 15 mM|
|Flow Rate||1.0 ml/min|
|Detection||UV, 270 nm|
|Class of Compounds||Hydrophobic, Ionizable|
|Analyzing Compounds||2-Aminobiphenyl, 4 -Aminobiphenyl|
Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.