4-CPA

4-CPA structural formula

CAS Number 122-88-3
Molecular Formula C8H7ClO3
Molecular Weight 186.590 g/mol
InChI Key SODPIMGUZLOIPE-UHFFFAOYSA-N
LogP 2.25
Synonyms
  • 4-Chlorophenoxyacetic acid
  • (4-Chlorophenoxy)acetic acid
  • Acetic acid, 2-(4-chlorophenoxy)-
  • 122-88-3
  • 4-06-00-00845
  • Acetic acid, (4-chlorophenoxy)-
  • (4-Chlorophenoxy)acetic acid
  • (p-Chlorophenoxy)acetic acid
  • Acetic acid, (p-chlorophenoxy)-
  • Acetic acid, 2-(4-chlorophenoxy)-
  • Acide 4-chlorophenoxyacetique
  • NSC 8769
  • NSC 9213
  • p-Chlorophenoxyacetic acid
  • Tomatone
  • Tomatotone
  • BRN 1211804
  • Caswell No. 204
  • EINECS 204-581-3
  • EPA Pesticide Chemical Code 019401
  • Marks 4-cpa
  • Parachlorophenoxyacetic acid
  • Sure-Set
  • Tomato Fix
  • Tomato hold
  • Kyselina 4-chlorfenoxyoctova
  • UNII-4EMM3U5P3K
  • 4-CPA
  • 4-Chlorophenoxyacetate
  • 4-Chlorphenoxyessigsaeure
  • para-chlorophenoxyacetic acid

Applications:


4-CPA Analysis on Obelisc and Primesep Mixed-Mode Columns
 
4-CPA (4-Chlorophenoxyacetic acid) is a synthetic pesticide that mimics plant hormones called auxins. A single residue method for the EURL (European Union Reference Laboratory) was developed for 4-CPA and other acidic compounds using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology. Retention of 4-CPA was controlled on both Obelisc R and a 3 micron Primesep 100 column of the same dimension.  By using a gradient for the organic and ionic components of the mobile phase, the method is capable of separating dozens of pesticides quickly while remaining LC/MS compatible.
 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds 4-CPA
 

Application Analytes:

4-CPA

HPLC Separation of Pesticides, Herbicides, Fungicides and Insecticides on Newcrom BH Column
Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom BH mixed-mode column. It can be used with different organic media such as acetonitrile (ACN) or methanol (MeOH). By varying the concentration of organic modifier and using different buffers like sulfuric acid (H2SO4), ammonium formate (AmFm), or formic acid, separation of desired pesticides can be achieved.

Condition

Column Newcrom BH, 3.2x100 mm, 5 µm, 100A
Mobile Phase MeCN, MeOH
Buffer H2SO4, Formic acid, AmFm pH 3.0
Flow Rate 0.6 ml/min
Detection UV 275 nm

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Monocrotophos, Metribuzin, Bromacil, Carbofuran, 4-Chlorophenoxyacetic acid, Bentazone, Folpet, Fenthion, Fenvalerate, Metrabuzin, Carbofuran, Atrazine, Parathion –methyl, MCPA, Clopyralid, Dicamba, Benthazon, Simazine, Diuron, MCPB, Mecoprop, 4-Chlorophenoxyacetic acid,2,4,5-T


Application Analytes:

2,4,5-Trichlorophenoxyacetic acid
4-CPA
Atrazine
Bentazone-sodium
Carbofuran
Clopyralid
Dicamba
Diuron
Fenvalerate
Folpet
MCPA
MCPB
Mecoprop
Methyl parathion
Monocrotophos
Simazine