4-Morpholinepropanesulfonic Acid (MOPS)

4-Morpholinepropanesulfonic Acid (MOPS) structural formula

CAS Number 1132-61-2
Molecular Formula C7H15NO4S
Molecular Weight 209.260 g/mol
LogP -1.99
  • 4-Morpholinepropanesulfonic acid
  • 3-(Morpholin-4-yl)propane-1-sulfonic acid
  • 1132-61-2
  • BRN 1106776
  • EINECS 214-478-5
  • 4-Morpholinepropane sulfonic acid
  • Morpholinopropanesulfonic acid
  • 3-Morpholinopropanesulfonic acid
  • 3-N-Morpholinopropanesulfonic acid
  • UNII-273BP63NV3


HPLC Separation of Morpholino Sulfates

MOPS and MES are buffers used in biology and biochemistry. They consist of a morpholine ring and propanesulfonic acid. Both molecules are zwitter-ionic and very polar. No retention can be achieved on reversed-phase columns. MOPS and MES are analyzed in HILIC mode on a Primesep N HPLC column. Other biological buffers can be analyzed with this general method. Biological buffers are not UV active and can be monitored by ELSD, CAD or LC/MS.

Application Analytes:

2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)

Effect of Buffer on HPLC Separation of Buffers

HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.

Application Analytes:

2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)
CAPS (3-(Cyclohexylamino)propanesulfonic acid)
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)