5-Aminosalicylic Acid

5-Aminosalicylic Acid structural formula

CAS Number 89-57-6
Molecular Formula C7H7NO3
Molecular Weight 153.137 g/mol
LogP 0.800
  • 5-Aminosalicylic acid
  • 5-Amino-2-hydroxybenzoic acid
  • Benzoic acid, 5-amino-2-hydroxy-
  • 89-57-6
  • 4-14-00-02058
  • Benzoic acid, 5-amino-2-hydroxy-
  • 2-Hydroxy-5-aminobenzoic acid
  • 3-Carboxy-4-hydroxyaniline
  • 5-Amino-2-hydroxybenzoic acid
  • 5-Aminosalicylsaure
  • Acide 5-aminosalicylique
  • acido 5-aminosalicilico
  • Asacolitin
  • Asacolon
  • Claversal
  • Fisalamine
  • Lixacol
  • m-Aminosalicylic acid
  • Mesacol
  • Mesalamine
  • Mesalazine
  • Mesasal
  • NSC 38877
  • Pentasa
  • Salicylic acid, 5-amino-
  • Salofalk
  • Salozinal
  • BRN 2090421
  • EINECS 201-919-1
  • Pentacol
  • UNII-4Q81I59GXC
  • Asacol HD
  • sfRowasa
  • Delzicol
  • 5-ASA
  • 5-Aminosalicylate
  • Mesalazina
  • Mesalazinum
  • p-Aminosalicylsaeure
  • 61513-32-4


HPLC Separation of Isomers of Amino Salicylic Acid
HPLC.cloud View on hplc.cloud

Condition 1

Column Newcrom A, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer H3PO4 - 0.5%
Flow Rate 0.5 ml/min
Detection UV 275 nm
  4-Aminosalicylic acid (PAS) is an antibiotic used in treatment of tuberculosis. It is a polar amino acid with limited retention on traditional C18 (reversed-phase) columns. Other isomers of aminosalicylic acid exist, but the main impurity in PAS is 5-Aminosalicylic acid, which also serves as anti-inflammatory drug. Both compounds are isomers with similar empirical structure and properties. These two isomers were separated on a Primesep 100 column with UV, ELSD and LC/MS compatible mobile phase. Method can be used a generic approach for separation of isomers of basic and zwitter ionic compounds. Isomers are retained and separated based on reversed-phase and cation-exchange properties. Retention time is controlled by the amount of acetonitrile, buffer concentration and buffer pH. Buffer pH is affecting ionization of these two compounds and thus serves as a powerful tool to adjust selectivity of separation.

Condition 2

Column Newcrom A, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer AmFm pH 3.0- 40 mM
Flow Rate 1.0 ml/min
Detection UV 256 nm,  MS-compatible mobile phase


Class of Compounds Acids
Analyzing Compounds 5-aminosalicylic acid, 4-aminosalicylic acid

Application Analytes:

4-Aminosalicylic Acid
5-Aminosalicylic Acid

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