5-Methyltetrahydrofolic Acid (5-MTHF)

CAS Number 134-35-0
Molecular Formula C20H25N7O6
Molecular Weight 459.464 g/mol
LogP -0.676
  • 5-Methyltetrahydrofolic acid
  • N-(4-{[(2-Amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl)methyl]amino}benzoyl)glutamic acid
  • Glutamic acid, N-[4-[[(2-amino-5,6,7,8-tetrahydro-4-hydroxy-5-methyl-6-pteridinyl)methyl]amino]benzoyl]-
  • 134-35-0
  • Methyl folate
  • 5-Methyl terahydrofolic acid
  • N5-Methyl-tetrahydrofolic acid
  • 5-Methyltetrahydropteroylglutamate
  • Prefolic A
  • N(sup 5)-Methyltetrahydrofolate


HPLC Separation of 5-Methyltetrahydrofolic Acid and Degradation Products

5-MTHF is a form of folic acid, one of the B vitamins. Folic acid is an essential vitamin used in synthesis of genetic molecules (DNA, RNA) and for the metabolism of amino acids. The separation of 5-MTHF can be achieved using reverse-phase (RP) Primesep SB column by hydrophobic, anion-exchange and ion-exclusion mechanisms. The method can be used for quality control (QC), research and development (R&D), etc. Fine tuning can be achieved with variations in mobile phase, pH and buffer selection. UV detection at 290mn. 

Application Analytes:

5-Methyltetrahydrofolic Acid (5-MTHF)