Acetaminophen (Paracetamol)

CAS Number 103-90-2
Molecular Formula C8H9NO2
Molecular Weight 151.165 g/mol
InChI Key RZVAJINKPMORJF-UHFFFAOYSA-N
LogP 0.5
Synonyms
  • Acamol
  • Acephen
  • Acetaco
  • Acetamidophenol
  • Acetaminophen
  • Acetominophen
  • Algotropyl
  • Anacin 3
  • Anacin-3
  • Anacin3
  • APAP
  • Datril
  • Hydroxyacetanilide
  • N-(4-Hydroxyphenyl)acetanilide
  • N-Acetyl-p-aminophenol
  • p-Acetamidophenol
  • p-Hydroxyacetanilide
  • Panadol
  • Paracetamol
  • Tylenol

Applications:


Separation of NyQuil Active Ingredients on Primesep C
Primesep C separates the active ingredients in the over-the-counter cold medicine, NyQuil. Succinate and bromate co-elute early, but acetaminophen, pseudoephedrine, doxylamine, and dextromethorphan are baseline resolved. A combination of cation exchange, complex formation, and hydrophobic interactions separate this mixture with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 205 nm.

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 205 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan


Application Analytes:

Acetaminophen (Paracetamol)
Dextromethorphan
Doxylamine
Pseudoephedrine (PSE)

HPLC Separation of Polar Drugs with MS-compatible Method
Acetaminophen is an over-the-counter pain and fever reducer, and a major component of cold and flue remedies. Phenylephrine is a decongestant. Guaifenesin is a mucolytic agent used to relieve respiratory difficulties. These three compounds of cough medication were separated by mixed-mode chromatography on a Primesep C HPLC column. Retention and order of elution for phenylephrine can be changed by buffer concentration and buffer pH. Method can be used for analysis of cough and cold composition during the production and in QC/QA environment. Method is compatible with LC/MS and can be used to analyze these components in biological fluids. This generic HPLC method is robust and reproducible.

Condition

Column Primesep C, 3x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 0.5 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Guaifenesin


Application Analytes:

Acetaminophen (Paracetamol)
Guaifenesin
Phenylephrine

Effect of pH on Retention of Basic Compounds on Primesep C Columns
Method for separation of components of cough medication shows how retention for acetaminophen, phenylephrine and guaifenesin is controlled. Order of elution for compounds can be changed based on composition of the mobile phase. The method is compatible with UV, ELSD and LC/MS and can be used for quantitation of drugs in formulation. Primesep C mixed-mode HPLC column is ideal candidate for analysis of cough compositions. Basic compounds are well retained in mixed-mode chromatography without use of ion-pairing reagents. Several companies validated this approach. The method is reproducible and robust and can be used in both production environments and R&D. This HPLC method can be adopted as general approach for analysis of basic compounds in various mixtures.

Condition

Column Primesep C, 3x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 0.5 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Guaifenesin


Application Analytes:

Acetaminophen (Paracetamol)
Guaifenesin
Phenylephrine

HPLC Separation of Acetaminophen, Phenylephrine, and Diphenhydramine
Primesep C separates acetaminophen (paracetamol), phenylephrine, and diphenhydramine-- common over-the-counter cold and flu medication ingredients such as Tylenol products—by either an acetonitrile or phosphoric acid gradient. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid with ultraviolet (UV) detection at 210 nm.

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Diphenhydramine


Application Analytes:

Acetaminophen (Paracetamol)
Phenylephrine

HPLC Separation of NyQuil Active Ingredients
NyQuil is a cough medication produced by Procter&Gamble. It consists of acetaminophen, pseudoephedrine, doxylamine and dextromethorphan. Most of the analytical HPLC methods include either use of ion-pairing reagent or a gradient elution. Mixed-mode chromatography allows to replace long gradient method with short isocratic method. All four compounds are well separated within 3-5 minutes. Methods can be optimized for analysis of other cough composition containing general cough suppressants and pain killers. Method is robust and being validated at several drug companies.

Condition

Column Primesep C, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Pseudoephedrine, Doxylamine, Dextromethorphan


Application Analytes:

Acetaminophen (Paracetamol)
Dextromethorphan
Doxylamine
Pseudoephedrine (PSE)

Separation of Drugs in Mixed-Mode HPLC
Obelisc R column can be successfully used for analysis of hydrophobic and hydrophilic basic and acidic drugs and corresponding counter-ions. This approach can be used for analysis of various cold and cough composition, pain killers, and other prescription and over-the-counter medications. Within pH 3-5, both cation- and anion mechanism are present on the column, along with hydrophobic interaction. Selectivity of this mixed-mode separation is controlled by the amount of acetonitrile, buffer concentration and buffer pH. Method is robust and reproducible, and can be used as a common HPLC method for analysis of drug molecules and compositions.

Condition

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan


Application Analytes:

Acetaminophen (Paracetamol)
Dextromethorphan

HPLC Separation of Drugs in Tylenol Cold and Cough Remedies
Components of Tylenol Cold and Cough Remedy are separated on Obelisc R mixed-mode column. Method can be used to determine compounds in various cough and cold compositions. Neutral (acetaminophen), basic (chlorpheniramine, dextromethorphan and pseudoephedrine) and acidic (maleic acid/Maleate) components are analyzed with perfect peak shape and retention control. Method can be used in production, QC/QA and biological studies for quantitation of various components in pharmaceutical formulations (Advil, Tylenol, Dimetapp, Robitussin, NyQuil, etc)

Condition

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable, Neutral, Basic
Analyzing Compounds Maleate, Chlorpheniramine, Dextromethorphan, Pseudoephedrine,  Acetaminophen


Application Analytes:

Acetaminophen (Paracetamol)
Chlorpheniramine
Dextromethorphan
Maleate
Pseudoephedrine (PSE)

USP Methods for the Analysis of an Analgesic Mixture Using the Legacy L1 Column
  Application Notes: Acetametaphin, aspirin, and caffeine tablets contain not less than 90 percent and not more than 110 percent of the labeled amounts if acetametaphin, asprin, and caffeine according the USP methods. USP HPLC method for separation of acetaminophen, aspirin and caffeine was developed on Legacy L1 column according to US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column contains C18 ligands. Support for the material is a spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP. Application Columns: Legacy L1 C18 HPLC column Application compounds: Acetaminophen, Aspirin, Caffeine, benzoic acid, and salicylic acid Mobile phase: MeOH/H2O/AcOH 28/69/3 Detection technique: UV Reference: USP30: NF35

Condition

Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 28/69/3
Buffer AcOH
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Hydrophobic, Ionizable
Analyzing Compounds Acetaminophen, Caffeine, Aspirin, Benzoic acid, Salicylic acid
 

Application Analytes:

Acetaminophen (Paracetamol)
Aspirin
Benzoic Acid
Caffeine

Separation of Acetaminophen and 4-Aminophenol on Primesep C
  Acetaminophen and 4-Aminophenol ar both aminophenols. Acetominophen or paracetamol is a widely used analgesic and antipyretic. 4-Aminophenol is an intermediate in the synthesis of paracetamol and used in photography as a developer. Both compounds were retained and resolved on Primesep C. Primesep C is a mixed-mode HPLC column which uses reversed-phase and unique complex-forming groups to resolve compounds that might otherwise coelute.  

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TEAPh
Flow Rate 1.0 ml/min
Detection UV, 210 nm

Description

Class of Compounds  Drug,  Hydrophilic, Ionizable
Analyzing Compounds Acetaminophen,  4-Aminophenol
 

Application Analytes:

4-Aminophenol
Acetaminophen (Paracetamol)

HPLC Analysis of Paracetamol
 

Paracetamol (also known as acetaminophen) is a mild analgesic commonly used for relief from headaches and used in many cold and flu medicines. Legacy L1 was used to retain Paracetamol by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

 

Condition

Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 15%
Buffer AmAc pH 4.0- 42 mM
Flow Rate 1.0 ml/min
Detection UV, 257 nm

Description

Class of Compounds  Drug,  Hydrophilic, Ionizable
Analyzing Compounds Paracetamol
 

Application Analytes:

Acetaminophen (Paracetamol)

HPLC Separation of Caffeine, Benzoic acid and Acetaminophen

Condition

Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 28%
Buffer Acetic Acid - 3%
Flow Rate 1.5 ml/min
Detection UV, 275 nm

Description

Class of Compounds  Drug, Acid,  Hydrophilic, Hydrophobic, Ionizable
Analyzing Compounds Caffeine, Benzoic acid, Acetaminophen
&

Application Analytes:

Acetaminophen (Paracetamol)
Benzoic Acid
Caffeine

HPLC Method for Analysis of Acetaminophen and Oxycodone
11 Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Oxycodone is semi-synthetic opioid type drug that is used to help relieve moderate to severe pain. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Acetaminophen and Oxycodone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Condition

Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Hydrocodone


Application Analytes:

Acetaminophen (Paracetamol)
Oxycodone

HPLC Method for Analysis of Vicodin (Acetaminophen & Hydrocodone)
78

Condition

Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm
   

Description

Class of Compounds Drug, Analgetic, Acid, Hydrophilic, Ionizable,
Analyzing Compounds Acetaminophen, Hydrocodone


Application Analytes:

Acetaminophen (Paracetamol)
Hydrocodone
Vicodin

HPLC Separation of Acetaminophen, Caffeine and Pyrilamine maleate


Acetaminophen is a p-aminophenol derivative with analgesic and antipyretic activities. It has weak anti-inflammatory properties, may cause liver, blood cell, and kidney damage. It is a nonprescription medication for mild-to-moderate pain and fever. Caffeine is a Central Nervous System (CNS) stimulant. It is an unregulated and legal drug in most parts of the world. It can be found in the seeds and leaves in a number of plants native in Africa, East Asia and South America. Pyrilamine maleate is a histamine H1 antagonist with hypnotic properties. It has multiple uses such as an anesthetic and is used against allergies. Newcrom R1, a column that takes advantage of the newest technologies, does not contain embedded acidic nor basic ionizable groups and can retain Acetaminophen, Caffeine and Pyrilamine maleate. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:

Acetaminophen (Paracetamol)
Caffeine
Maleate
Pyrilamine
Pyrilamine maleate