|Molecular Weight||60.052 g/mol|
Acetic acid and acetic hydrazide are quantitated in one run using a Primesep N HILIC mixed-mode column. Method can be used for determination of acetic acid and acetic hydrazide by HPLC.
Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.