Acetic Acid

Acetic Acid structural formula

CAS Number 64-19-7
Molecular Formula C2H4O2
Molecular Weight 60.052 g/mol
InChI Key QTBSBXVTEAMEQO-UHFFFAOYSA-N
LogP -0.170
Synonyms
  • Acetic acid
  • 64-19-7
  • Vinegar
  • 4-02-00-00094
  • Acetic Acid Glacial
  • ACETIC ACID, GLACIAL
  • Acide acetique
  • Aci-Jel
  • ESSIGSAEURE UEBER 10 BIS 40%
  • ESSIGSAEURE UEBER 40%
  • Essigsaure
  • Ethanoic acid
  • Ethanoic acid monomer
  • Ethylic acid
  • Glacial acetic acid
  • Methanecarboxylic acid
  • NSC 111201
  • NSC 112209
  • NSC 115870
  • NSC 127175
  • NSC 132953
  • NSC 406306
  • UN 2789
  • UN 2790
  • Vinegar acid
  • BRN 0506007
  • Caswell No. 003
  • EINECS 200-580-7
  • EPA Pesticide Chemical Code 044001
  • FEMA No. 2006
  • FEMA Number 2006
  • Pyroligneous acid
  • Acetic acid 0.25% in plastic container
  • Acido acetico
  • Azijnzuur
  • Octowy kwas
  • Aceticum acidum
  • AcOH
  • Acetate
  • CH3-COOH
  • CH3CO2H
  • E 260
  • E-260
  • E260
  • Essigsaeure
  • Ethanoate
  • Ethylate
  • Glacial acetate
  • HOAc
  • INS No. 260
  • Kyselina octova
  • MeCO2H
  • MeCOOH
  • Methanecarboxylate
  • Methanecarboxylic acid
  • ethoic acid
  • 1053656-97-5
  • 77671-22-8

Applications:


HPLC Separation of Acetic acid and Acetic Hydrazide


Acetic acid and acetic hydrazide are quantitated in one run using a Primesep N HILIC mixed-mode column. Method can be used for determination of acetic acid and acetic hydrazide by HPLC.



Application Analytes:

Acetic Acid
Acetic Hydrazide

HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column
Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.  

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 60%
Buffer H2SO4 - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description

Class of Compounds  Acid, Hydrophilic, Ionizable
Analyzing Compounds Trifluoroacetic acid (TFA), Acetic acid (AcOH)
 

Application Analytes:

Acetic Acid
TFA (Trifluoroacetic Acid)

HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid,   Trichloroacetic acid
HPLC.cloud View on hplc.cloud Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 50/50%
Buffer H2SO4 - 0.2%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA),   Trichloroacetic acid


Application Analytes:

Acetic Acid
Chloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid

HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column
HPLC.cloud View on hplc.cloud Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry.  Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns.  By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.  UV detection at 200nm.

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H2SO4 - 0.3%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Acetic acid, Chloroacetic acid,  Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid


Application Analytes:

Acetic Acid
Bromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid