Adenosine Monophosphate

CAS Number 61-19-8
Molecular Formula C10H14N5O7P
Molecular Weight 347.224 g/mol
LogP -1.78
  • Adenosine-5-phosphate
  • 5'-Adenylic acid
  • 61-19-8
  • 4-26-00-03615
  • 5'-Adenylic acid
  • Adenosine 5'-(dihydrogen phosphate)
  • Adenosine 5'-monophosphate
  • Adenosine 5'-phosphate
  • Adenosine 5'-phosphoric acid
  • Adenosine monophosphate
  • adenosine phosphate
  • Adenosine-5-monophosphoric acid
  • Adenosine-5'-phosphate(AMP)
  • Adenosinphosphat
  • Adenovite
  • Adenylic acid
  • Cardiomone
  • fosfato de adenosina
  • Lycedan
  • My-B-Den
  • NSC 20264
  • Phosaden
  • Phosphaden
  • Phosphate d'adenosine
  • Phosphentaside
  • Adenosine 5'-monophosphoric acid
  • AMP (VAN)
  • BRN 0054612
  • EINECS 200-500-0
  • Ergadenylic acid
  • Myoston
  • NSC-20264
  • Muskeladenosin-phosphorsaeure
  • Muskeladenylsaeure
  • Monophosphadenine
  • Adenosini phosphas
  • UNII-415SHH325A
  • 5'-Adenylate
  • 5'-O-phosphonoadenosine
  • Adenosine 5'-phosphorate
  • Adenosine-5'-monophosphorate
  • Adenosine-5-monophosphorate
  • Adenosine-monophosphate
  • Adenosine-phosphate
  • Ado5'P
  • Muscle adenylate
  • Muscle adenylic acid
  • My-beta-Den
  • PAdo
  • [(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxyphosphonic acid
  • [(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl]methoxyphosphonic acid
  • adenosine-5'P
  • pA
  • {[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}phosphonic acid
  • 162756-82-3
  • 47286-65-7
  • 47287-97-8
  • 53624-78-5
  • 67583-85-1
  • 697214-87-2


HPLC Separation of Adenosine Mono-, Di- and Triphosphate in Reversed-Phase Mixed-Mode with LC/MS Compatible Conditions
Adenosine mono-, di and triphosphate are hydrophilic nucleotides which serve as building blocks of DNA and RNA. Each molecule consists of phosphate or phosphate groups, adenine and sugar ribose. Molecules are hydrophilic and lack a retention mechanism on traditional reversed-phase column. Three nucleotides were retained and separated on Primesep B2 reversed-phase anion-exchange column. retention time is controlled by buffer concentration and buffer pH. ADP and ATP require higher concentration of buffer to facilitate elution. Method can be used for LC/MS analysis of different nucleotides in various sample matrices (biofluids, plasma, blood, urine). Other detection techniques can be used for analysis. Method is reliable and robust and can tolerate interference from sample matrix. Additional sample preparation might be required.  


Column Primesep B2, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 2.9- 5-20 mM 3 min, 20-150 mM
Flow Rate 0.5 ml/min
Detection ELSD


Class of Compounds Nucleotide, Hydrophilic, Ionizable
Analyzing Compounds Adenosine Monophosphate, Adenosine Diphosphate, Adenosine Triphosphate

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate

Separation of Nicotinamide and Related Substances

A complex mixture of nicotinamide and related impurities was separated on Obelisc R mixed-mode column. Nicotinamide, methylnicotinamide, nicotinamide adenine mononucleotide, nicotinamide adenine dinucleotide, and adenosine monophosphate were baseline resolved in a 15 minute long method. This mixed-mode approach can be used for analysis of other nucleotides. Obelisc R trimodal column separates this complex mixture based on reversed-phase, cation-exchange and anion-exchange mechanisms. Retention is controlled by amount of ACN, buffer concentration and buffer pH. Additional selectivity can be gained by exploring various buffers within the same pH

Application Analytes:

Adenosine Monophosphate
Nicotinamide Adenine Dinucleotide
Nicotinamide Adenine Mononucleotide

Separation of Nine Nucleotides by Mixed-Mode Chromatography
  Nucleotides are important biological molecules which serve as subunits of nucleic acids. They are composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Nucleotides cannot be retained by reverse-phase chromatography without an ion-pairing reagent due to their highly polar nature. Primesep SB is capable of retaining and separating nine nucleotides. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups.

Application Analytes:

Adenosine Diphosphate
Adenosine Monophosphate
Adenosine Triphosphate
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate
Guanosine Diphosphate
Guanosine Monophosphate
Guanosine Triphosphate

Separation of Nucleotide Monophosphates


Nucleotides are the monomers of DNA and RNA, composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Primesep SB is a good column for separating these highly polar compounds. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups. Retention can me manipulated by adjusting acetonitrile, and the baseline separation can be achieved in under 6 minutes using a gradient. 

Application Analytes:

Adenosine Monophosphate
Cytidine Monophosphate
Guanosine Monophosphate
Inosine Monophosphate
Uridine Monophosphate