|Molecular Weight||239.316 g/mol|
Primesep 100 separates albuterol and amoxicillin with baseline resolution by a combination of cation-exchange, hydrophobic, and polar interactions. The mass spec (LC/MS) compatible separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.
Application Notes: Albuterol is a well known drug used to treat bronchospasms. Albuterol come in many different forms, ranging from nebulizer to tablets. Albuterol is an important drug in combatting breathing problems. The USP methods for albuterol dictate that albuterol should not contain less than 98.5% and no more than 101% calculated on a dried basis. The USP HPLC method for the separation of albuterol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of USP.
Application Columns: Legacy L1 C18 HPLC column
Application compounds: Albuterol
Mobile phase: Water with 1% acetic acid and 5mM hexane sulfonic acid and MeOH (60:40)
Detection technique: UV
Reference: USP35: NF30