Primesep 100 separates albuterol and amoxicillin with baseline resolution by a combination of cation-exchange, hydrophobic, and polar interactions. The mass spec (LC/MS) compatible separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with UV detection at 210 nm.
USP Method for the Analysis of Amoxicillin using the Legacy L1 Column
Application Notes: Amoxicillin is one of the most commonly prescribed antibiotics. It is often used for treating strep throat. According to USP methods, amoxicillin should not contain less than 900ug and no more than 1050ug of amoxicillin per mg. The USP HPLC method for the separation of amitriptyline was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.Application Columns: Legacy L1 C18 HPLC column
Application compounds: Amoxicillin
Mobile phase: MeCN/50 mM dibasic sodium phosphate
Detection technique: UV
Source: USP35: NF30
High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin and Cephalexin.
Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Both compounds can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.