Arginine

CAS Number 7200-25-1
Molecular Formula C6H14N4O2
Molecular Weight 174.204 g/mol
InChI Key ODKSFYDXXFIFQN-UHFFFAOYSA-N
LogP -3.05
Synonyms
  • Arginine
  • 7200-25-1
  • (.+-.)-Arginine
  • Arginine, DL-
  • DL-Arginin
  • DL-arginina
  • DL-arginine
  • EINECS 230-571-3
  • (+-)-Arginine
  • UNII-FL26NTK3EP
  • 2-amino-5-(carbamimidamido)pentanoic acid
  • 2-amino-5-guanidinopentanoic acid
  • Arginin
  • Harg

Applications:


Amino Acids Analysis in Acid Gradient Condition
11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer TFA , gradient  0.05-0.3 % , 25 min
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Gaspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine
 

Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

HPLC Separation of Amino Acids in Supplements Capsules



Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.



Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine

HPLC Separation of Citrulline and Arginine
               
Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.

Condition

Column Primesep 200,  4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Citrulline, Arginine


Application Analytes:

Arginine
Citrulline
Sodium

HPLC Analysis of Active Drug and Amino Acids in a Formulation
Polar amino acids are very often used as components of vitamin and supplement composition. Analysis of such complex composition is a challenging task. In this application, 5 amino acids (asparagine, glutamic acid, proline and arginine) and two preservatives (methyl paraben and propyl paraben) are separated on a Primesep 100 reversed-phase cation-exchange column with LC/MS compatible mobile phase. Method does not require ion-pairing reagent in the mobile phase. Compounds are monitored by ELSD and UV. Method is validated for quantitation of underivatized amino acids in complex mixtures. The method is simple and robust and can be used for analysis of various vitamin formulations.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD 50C, UV 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid, Aspargine,  Proline, Lysine, Arginine, Methyl paraben, Propyl paraben
 

Application Analytes:

Arginine
Asparagine
Ethyl Paraben
Glutamic Acid
Lysine
Methylparaben
Proline

HPLC Analysis of Citrulline and Arginine on Primesep Columns
Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.

Condition

Column Primesep, 2.1x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 0.2 ml/min
Detection ELSD
 

Description

Class of Compounds Ions,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Citrulline, Arginine, Chloride


Application Analytes:

Arginine
Citrulline

HPLC Separation of Lysine and Arginine from Other Amino Acids
  Application Notes: Amino acids are polar ionic compounds which are not retained on reversed-phase column without ion-pairing reagent. In our application, lysine and arginine can be separated from other amino acids. Amino acids with a pH between 3 and 5 and with one basic and one acidic group become very polar. Therefore these amino acids don’t have strong ion-exchange interaction with Primesep C stationary phase. Amino acids with two amino groups still carry positive net charge and can interact with stationary phase by cations-exchange mechanism. pH variation of the mobile phase can be an effective tool to adjust selectivity of separation for zwitter-ionic, basic and acidic compounds. This method can be used for separation of mono-charged compounds from compounds having an extra charge. Application Columns: Primesep C Application compounds: Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep C, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 15%
Buffer AmAc pH 5.0- 15 mM
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Aspartic acid, Glutamic acid, Aspargine, Glycine, Proline, Alanine, Phenylalanine, Tyrosine, Leucine, Lysine, Arginine
 

Application Analytes:

Alanine
Arginine
Asparagine
Aspartic Acid
Glutamic Acid
Glycine
Leucine
Lysine
Phenylalanine
Proline
Tyrosine

HPLC Method for Analysis of L-Lysine and L-Arginine
l-Lysine , L-Arginine Chr_1106 L-Lysine is an essential amino acid required for growth and tissue repair, it can also help with anxiety. The amino acid is most often found in red meats,beans, fish, and dairy products. L-Arginine is an essential amino acid that is used to treat congestive heart failure, combat fatigue and circulatory diseases. It is also used to stimulate the immune system. Obelisc R is a reverse-phase column. It contains embedded ionic groups and can retain L-Lysine and L-Arginine. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Analytes:

Arginine
D-Lysine
Lysine
N-Nitro-L-arginine

HPLC Separation of Mixture of Conditionally Essential Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

Amino Acids
Arginine
Cysteine
Glycine
L-Cysteine
L-Glutamine
Proline
Tyrosine

HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column
 

Condition

Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
Leucine
Methionine
Phenylalanine
Proline
Tryptophan
Valine