Benzenesulfonamide

CAS Number	98-10-2
Molecular Formula	C6H7NO2S
Molecular Weight	157.191 g/mol
InChI Key	KHBQMWCZKVMBLN-UHFFFAOYSA-N
LogP	0.31
Synonyms	Benzenesulfonamide 98-10-2 4-11-00-00050 bencenosulfonamida benzenesulphonamide Benzolsulfonamid M and B 7973 NSC 5341 NSC 85506 Phenylsulfonamide Benzolsulfonamide Benzosulfonamide BRN 1100566 EINECS 202-637-1

Applications:

Separation of Sulfonamides and Phenylhydrazine by Mixed-Mode HPLC

Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.

Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide
Sulfonamides

HPLC Separation of Aromatic Sulfonamides and Hydrozine

Benzenesulfonamide
Chlorobenzenesulfonamide
Hydroxybenzenesulfonamide
Hydroxysulphaminophenylhydrazine
Sulfanilamide

HPLC Separation of Benzenesulfonamide and Sulfanilamide

Both Benzenesulfonamide and sulfanilamide are organic antibacterial compounds differing only in the addition of an extra amino group in sulfanilamide. The closely related compounds can be separated on a mixed-mode Primesep D column by hydrophobic and anion-exchange mechanisms or Primesep 100 column by hydrophobic and cation-exchange mechanisms with the elution order reversed using identical mobile phases. UV Detection at 230nm.

Benzenesulfonamide
Sulfanilamide