CAS Number | 98-10-2 |
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Molecular Formula | C6H7NO2S |
Molecular Weight | 157.191 g/mol |
InChI Key | KHBQMWCZKVMBLN-UHFFFAOYSA-N |
LogP | 0.31 |
Synonyms |
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Primesep 100 separates a mixture of sulfonamides (benzenesulfonamide, chlorobenzenesulfonamide, hydroxybenzenesulfonamide) and hydroxysulphaminophenylhydrazine by a mixture of polar and hydrophobic interactions. The stationary phase’s hydrophobic functionality provides a reversed-phase mechanism while the embedded cation-exchange group provides polar interactions. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm. This method is mass spec (LC/MS) and evaporative light scattering (ELSD) compatible.
Column | Newcrom А, 3.2x50 mm, 3 µm, 100A |
Mobile Phase | MeOH/H2O - 80/20% |
Buffer | H2SO4 - 0.005% |
Flow Rate | 0.5 ml/min |
Detection | 275 nm |
Column | Primesep D, , Primesep 100 4.6x150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O - 10/90% |
Buffer | Formic Acid - 0.1% |
Flow Rate | 0.5 ml/min |
Detection | UV 230 nm |