Benzenesulfonic Acid

CAS Number 98-11-3
Molecular Formula C6H6O3S
Molecular Weight 158.170 g/mol
InChI Key SRSXLGNVWSONIS-UHFFFAOYSA-N
LogP -0.495
Synonyms
  • Benzenesulfonic acid
  • 98-11-3
  • 4-11-00-00027
  • acide benzenesulfonique
  • acido bencenosulfonico
  • Benzenemonosulfonic acid
  • benzenesulphonic acid
  • Benzensulfonic acid
  • BENZOLSULFONSAEURE
  • Benzolsulfonsaure
  • Besylic acid
  • Phenylsulfonic acid
  • PHENYLSULPHONIC ACID
  • Benzene sulphonic acid
  • BRN 0742513
  • EINECS 202-638-7
  • Kyselina benzensulfonova
  • UNII-685928Z18A
  • Blu-gard snitizing teat dip

Applications:


HPLC Separation of Benzenesulfonic and p-Toluenesulfonic Acids


Benzenesulfonic and toluenesulfonic acids are organic acids used in pharmaceutical industry as counter-ions for basic drugs. Both acids are hydrophilic in nature with strong acidic properties. Obtaining separation, retention and good peak shape with traditional reversed-phase columns is a challenging task. We developed robust mixed-mode approach for separation of benzenesulfonic acid and p-toluenesulfonic acid on a Primesep D reversed-phase anion-exchange column. Elution of these compounds is controlled by the amount of ions in the mobile phase and amount of acetonitrile. Both compounds are separated within 4 minutes and can be monitored by UV, ELSD, CAD or LC/MS. This column can be also used to analyze hydrophobic basic drugs by reversed-phase cation-exclusion mechanisms.



Application Analytes:

Benzenesulfonic Acid
Organic Acids
p-Toluenesulfonic Acid (PTSA)

HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography
The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.

Application Analytes:

Benzenesulfonic Acid
Bromide
Chlorate
Chloride
Citric Acid
Dextromethorphan
Fumaric Acid
Maleic Acid
Organic Acids
Perchlorate
Phosphoric Acid
Pyrilamine
Succinic Acid
Tartaric Acid
Verapamil
p-Toluenesulfonic Acid (PTSA)

The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep B2 mixed-mode column. Primesep B2 is a reverse-phase column with embedded basic ion-pairing groups.
The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep B2 mixed-mode column. Primesep B2 is a reverse-phase column with embedded basic ion-pairing groups.

Application Analytes:

Benzenesulfonic Acid
Butanesulfonic acid
Glucuronic acid
Methanesulfonic Acid