Benzylamine

CAS Number 100-46-9
Molecular Formula C7H9N
Molecular Weight 107.156 g/mol
InChI Key WGQKYBSKWIADBV-UHFFFAOYSA-N
LogP 1.09
Synonyms
  • Benzylamine
  • 1-Phenylmethanamine
  • Benzenemethanamine
  • 100-46-9
  • 4-12-00-02155
  • Benzenemethanamine
  • (Aminomethyl)benzene
  • (Phenylmethyl)amine
  • 1-Phenylmethanamine
  • bencilamina
  • Benzylamin
  • Monobenzylamine
  • N-Benzylamine
  • NSC 8046
  • Phenylmethanamine
  • α-Aminotoluene
  • ω-Aminotoluene
  • BRN 0741984
  • EINECS 202-854-1
  • Moringine
  • UNII-A1O31ROR09
  • .omega.-aminotoluene
  • 1utj
  • 1utn
  • 2bza
  • ABN
  • Aminotoluene
  • Benzenemethanamine, 9CI
  • Phenylmethanamine
  • Quadrapure(TM) benzylamine
  • Quadrapure(TM) bza
  • Sumine 2005
  • Sumine 2006
  • Toluene,alpha-amino
  • a-Aminotoluene
  • alpha-aminotoluene
  • laquo Omegaraquo -aminotoluene
  • moringine
  • omega-aminotoluene
  • 857483-23-9
  • 858831-93-3

Applications:


Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds



Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection.



Application Analytes:

Amino Acids
Benzoic Acid
Benzonitrile
Benzylamine
Mandelic Acid
Organic Acids
Phenylalanine
Pyridine
Toluene
Tyrosine

Control of Cation Exchange Properties by pH of Primesep C Column



Primesep C demonstrates the control of ion-exchange properties of Primesep columns. The peak order of caffeine and benzylamine in a mixture of caffeine, benzylamine, and phenol reverses when the mobile phase modifier is changed from ammonium acetate to trifluoroacetic acid. Control of the separation is possible even with mass spec (LC/MS) compatible mobile phases of water, acetonitrile (MeCN, ACN) and ammonium acetate or trifluoroacetic acid (TFA).



Application Analytes:

Benzylamine
Caffeine
Phenol

Neutral, Base, and Acid on Primesep A Column



Primesep A separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.



Application Analytes:

Benzonitrile
Benzylamine
Maleic Acid

Neutral, Base, and Acid on Primesep 100 Column



Primesep 100 retains and separates an acid, base, and neutral in one HPLC injection. Maleic acid, benzylamine, and benzonitrile are resolved by ion-exclusion, ion-exhange and reversed-phase modes. The separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.



Application Analytes:

Benzonitrile
Benzylamine
Maleic Acid

Neutral, Base, and Acid on Primesep 200 Column



Primesep 200 separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.



Application Analytes:

Benzonitrile
Benzylamine
Maleic Acid

Neutral, Base, and Acid on Primesep B Column



Primesep B separates acids, bases, and neutrals in one injection. Maleic acid, benzonitrile, and benzylamine are baseline resolved by a combination of reversed-phase, ion-exchange, and ion-exclusion mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 210 nm.



Application Analytes:

Benzonitrile
Benzylamine
Maleic Acid

Primesep C Column Retention Control



Primesep C separates benzylamines with an easily tunable HPLC method that allows retention control. Benzylamine, N-methylbenzylamine, and N,N-dimethylbenzylamine peak order and retention can be significantly changed by altering the water/acetonitrile ratio in the mobile phase which changes the combination of cation exchange, complex formation, and hydrophobic interactions. This mixture is separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 210 nm.



Application Analytes:

Benzylamine
N,N-Dimethylbenzylamine
N-Methylbenzylamine

HPLC Separation of Acidic, Basic and Zwitterionic Compounds on Special Columns for Retention of Polar Compounds


Primesep mixed-mode columns are designed to separate basic, acidic, neutral and zwitter-ionic compounds. Elution of these compounds is facilitated by acetonitrile and ions in the mobile phase. In all cases the retention time on mixed-mode columns is significantly longer than on traditional reverse-phase columns. Presence of cation-exchange mechanism in Primesep 100 column allows to achieve better retention and peak shape for analytes like phenylalanine and benzylamine. Various buffers can be used along with different detection techniques. This HPLC method can be adopted as a universal approach for analysis of basic, acidic and zwitter-ionic compounds in complex mixtures and formulations. Compounds can be monitored by UV, ELSD, CAD and LC/MS.



Application Analytes:

Benzoic Acid
Benzylamine
Phenylalanine
Zwitterion

HPLC Separation of 8 Generic Compounds on Primesep Columns
Mixed-mode HPLC columns allow to analyze compounds with drastically different properties in one run. Acidic, basic, and neutral compounds can be separated in one run using either isocratic or gradient conditions. In this application, neutral hydrophilic (uracil, phenol and hydroquinone), neutral hydrophobic (toluene), hydrophilic acidic (benzoic acid), hydrophilic basic (lutidine) and hydrophobic basic (amitriptyline) are separated using gradient of ACN. Neutral compounds are retained by reversed-phase mechanism, hydrophilic acidic compound become more hydrophobic at lower pH and retain by reversed-phase mechanism too. Basic compounds are retained by cation exchange mechanism, and hydrophobic basic compounds are retained by reversed-phase and cation-exchange mechanisms. All compounds are resolved within 17 minutes on a short column. Method can be applied to various polar and hydrophobic compounds, which can be separated on one column and in one run. Mixed-mode columns can operate in single or combination of several modes: reversed-phase, ion-exchange, ion-exclusion and HILIC. This mixed-mode HPLC column can be used as a general column for separation of wide range of compounds.

Application Analytes:

Amitriptyline
Benzoic Acid
Benzylamine
Hydroquinone
Lutidine
Phenol
Toluene
Uracil

HPLC Separation of Acidic, Basic, and Neutral Compounds


Primesep 100 and Primesep 200 columns can be used as a universal column for analysis of wide range of compounds. These mixed-mode reversed-phase ion-exchange HPLC columns can provide a valuable alternative to traditional reversed-phase column. Amines, amino acids, quaternary amines, and various zwitter-ions can be analyzed along with hydrophobic compounds and organic and inorganic counter-ions. In this application, 8 compounds with different hydrophobic, hydrophilic, basic and acidic properties are separated based on their properties. Primesep 100 column is a mixed-mode HPLC column with a C12 carbon chain and carboxylic acid on the surface with pKa of 1. Primesep 200 column is a mixed-mode HPLC column with a C12 carbon chain and carboxylic acid on the surface with pKa of 2. These columns can be used with 100% organic (ACN) and 100% aqueous mobile phases. This HPLC method can be adopted as a generic and robust approach for analysis of acidic, basic and neutral compounds within the same run.



Application Analytes:

Amitriptyline
Benzoic Acid
Benzylamine
Hydroquinone
Lutidine
Phenol
Toluene
Uracil

Effect of mobile phase composition on retention of 3 compounds on Obelisc R


This application shows the effect of mobile phase composition on retention of acidic, basic and neutral compound. Retention of basic and acidic compounds is controlled by buffer pH and concentration, while retention of neutral compound is controlled by the amount of acetonitrile. Buffer pH changes not only ionization state of ionizable basic and acidic compounds, but ionization state of trimodal stationary phase Obelisc R. Available detection techniques include UV, ELSD, LC/MS, and Corona.



Application Analytes:

Benzoic Acid
Benzonitrile
Benzylamine

HPLC Separation of Amino Acids, Bases, Acids, and Neutrals on Obelisc R

Condition

Column Obelisc R, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer AmAc 10 mM pH 4.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Bases, Neutral, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Amino acids
 

Application Analytes:

2,6-Lutidine
Benzoic Acid
Benzonitrile
Benzylamine
Phenol
Phenylalanine
Pyridine
Toluene
Tryptophan

Generic Screening Method for Complex Mixtures



Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil