beta-Aminobutyric Acid

beta-Aminobutyric Acid structural formula

CAS Number 541-48-0
Molecular Formula C4H9NO2
Molecular Weight 103.122 g/mol
InChI Key OQEBBZSWEGYTPG-UHFFFAOYSA-N
LogP -1.79
Synonyms
  • beta-Aminobutyric acid
  • 3-Aminobutanoic acid
  • Butanoic acid, 3-amino-
  • 541-48-0
  • BABA
  • EINECS 208-783-2
  • EINECS 220-617-0
  • UNII-4282SA5CTS
  • 3-Aminobutyric acid
  • 3-aminobutanoic acid
  • 3-methyl-beta-alanine
  • 167222-96-0
  • 2835-82-7

Applications:


HPLC Separation of Isomers of Aminobutyric Acids
Primesep C separates the isomers of aminobutyric acids by a combination of reversed-phase and ionic interaction mechanisms. alpha-Aminobutyric acid, beta-Aminobutryic acid, and gamma-Aminobutyric acid (GABA) are baseline resolved without ion-pair reagents. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) ammonium acetate with evaporative light scattering detection (ELSD).

Condition

Column Primesep C,  3.2x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.1
Flow Rate 0.5 ml/min
Detection ELSD
 

Description

Class of Compounds Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)


Application Analytes:

Aminobutyric Acids
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

HPLC Separation of alpha-Aminobutyric, beta-Aminobutyric, and gamma-Aminobutyric acids on Obelisc N
GABA (neurotransmitter) and its isomers are polar zwitter-ionic compounds. Due to the position of amino-groups, all three compounds show different polar and basic properties. The isomers of aminobuturic acid are separated on an Obelisc N HILIC/cation-exchange column. Buffer concentration has a different effect on retention of alpha-, beta-, and gamma-aminobutyric acid. This general and robust method can be used for separation of other polar and ionizable compounds and isomers by mixed-mode chromatography.

Condition

Column Obelisc N,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds Acid
Analyzing Compounds Alpha-Aminobutyric acid, Beta-Aminobutryic acid, Gamma-Aminobutyric acid (GABA)


Application Analytes:

Zwitterion
alpha-Aminobutyric Acid
beta-Aminobutyric Acid
gamma-Aminobutyric Acid (GABA)

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