Cetylpyridinium Chloride

Cetylpyridinium Chloride structural formula

CAS Number123-03-5
Molecular FormulaC21H38ClN
Molecular Weight339.990
InChI KeyYMKDRGPMQRFJGP-UHFFFAOYSA-M
LogP2.60
Synonyms
  • Cetylpyridinium chloride
  • 1-Hexadecylpyridin-1-ium chloride
  • Pyridinium, 1-hexadecyl-, chloride (1:1)
  • 123-03-5
  • Pyridinium, 1-hexadecyl-, chloride
  • 1-Cetylpyridinium chloride
  • 1-Hexadecylpyridinium chloride
  • 1-n-Hexadecanepyridinium chloride
  • 1-Palmitylpyridinium chloride
  • Acetoquat CPC
  • Aktivex
  • Ammonyx CPC
  • Biosept
  • Ceepryn chloride
  • Cepacol
  • Cepacol chloride
  • Cetafilm
  • Cetamium
  • Cetylpyridiniumchlorid
  • Cetylpyridinum bromide
  • Chlorure de cetylpyridinium
  • cloruro de cetilpiridinio
  • Dobendan
  • Hexadecylpyridinium chloride
  • Intexsan CPC
  • Medilave
  • Merocet
  • Merothol
  • N-Cetylpyridinium chloride
  • N-CETYLPYRIDINIUM-CHLORID
  • Newkalgen B 651P
  • n-Hexadecylpyridinium chloride
  • Pionin B 651P
  • Pristacin
  • Pyridinium, 1-hexadecyl, chloride
  • Pyridinium, 1-hexadecyl-, chloride (1:1)
  • PYRIDINIUM, CETYL-, CHLORIDE
  • Pyrisept
  • Quaternario CPC
  • Caswell No. 166A
  • Ceeprin chloride
  • Cetyl pyridinium chloride
  • Cetylpyridini chloridum
  • EINECS 204-593-9
  • EPA Pesticide Chemical Code 069160
  • NSC 14864
  • Swabettes Hoechst
  • Cetilpiridinio cloruro
  • Cetylpyridinii chloridum
  • UNII-6BR7T22E2S
  • cetylpyridinium chloride anhydrous
  • 136499-13-3
  • 27841-61-8
  • 50958-35-5
  • 951742-58-8

Applications:

HPLC Method for Separation of Hydrotopic, Cationic and Anion Surfactants on Newcrom BH Column

July 10, 2023

HPLC Method for Analysis of Hydrotopic, Cationic and Anion Surfactants on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Hydrotopic, Cationic and Anion Surfactants

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Hydrotropic Surfactants: While not a category of charge like the others, hydrotropic surfactants are a distinct class that promote the solubility of other solutes in water. Sodium xylene sulfonate and sodium cumene sulfonate are examples of hydrotropes.

The selection of a specific type of surfactant depends on the specific application and the properties of the surfactant. Some surfactants can be aggressive and irritating to skin or eyes (like certain anionic surfactants), while others are milder. Similarly, some are better at emulsifying oil or suspending dirt, while others might provide good foaming action or work better in hard water.

All compounds can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide

Condition

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -40-80%, 10 min
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsSurfactants
Analyzing CompoundsBenzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1-Ethylpyridinium bromide
1-Octanesulfonic acid
1-Pentanesulfonic acid
Benzalkonium chloride
Cetylpyridinium Chloride
Dodecanoic acid (Lauric acid)
Sodium dodecyl sulfate
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column

July 10, 2023

HPLC Method for Analysis of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants on Newcrom BH Column
HPLC Method for Separation of Hydrotopic, Cationic, Nonionic and Anion Surfactants

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Hydrotropic Surfactants: While not a category of charge like the others, hydrotropic surfactants are a distinct class that promote the solubility of other solutes in water. Sodium xylene sulfonate and sodium cumene sulfonate are examples of hydrotropes.

The selection of a specific type of surfactant depends on the specific application and the properties of the surfactant. Some surfactants can be aggressive and irritating to skin or eyes (like certain anionic surfactants), while others are milder. Similarly, some are better at emulsifying oil or suspending dirt, while others might provide good foaming action or work better in hard water.

All compounds can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Condition

ColumnNewcrom BH, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -40-80%, 30 min
BufferAmmonium formate pH 3.0 – 20 mM
Flow Rate1.0 ml/min
DetectionELSD, 50C

Description

Class of CompoundsAliphatic sulfonic acid
Analyzing CompoundsBenzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
1-Decanesulfonic acid
1-Heptanesulfonic acid
1-Hexanesulfonic acid, sodium salt
1-Octanesulfonic acid
1-Pentanesulfonic acid
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium dodecyl sulfate
Triton X100
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Separation of Chlorhexidine and Cetylpyridinium Chloride on Primesep B Column

January 22, 2020

HPLC Separation of Chlorhexidine and Cetylpyridinium chloride on Primesep B Column_1222

Chlorhexidine gluconate, or simply chlorhexidine, is a biguanide used as an antiseptic and disinfectant. It is a component of mouthwash rinses that has been shown to reduce plaque, gingivitis and oral bacteria. It’s also used as a topical agent for skin disinfection. Cetylpyridinium chloride is another type of antiseptic used in mouthwash rinses. Both compounds are cationic. They can be separated using HPLC on SIELC’s reverse-phase (RP) mixed-mode Primesep B column with the mobile phase of acetonitrile (ACN) and water with formic acid buffer and UV detected at 270nm.

Condition

Column Primesep B,  3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid – 0.5%
Flow Rate 0.5 ml/min
Detection UV 270 nm

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Chlorhexidine, Cetylpyridinium Chloride

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Cetylpyridinium Chloride
Chlorhexidine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Cetylpyridinium Chloride and Triethylene Glycol on Obelisc N Column

July 8, 2011

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Cetylpyridinium chloride is hydrophobic basic compound and triethylene glycol is hydrophilic neutral compounds. Quantitative analysis of both compounds is problematic due to a different nature of these two analytes. Both compounds were analyzed on an Obelisc N column in HILIC/cation-exchange mode. Cetylpyridinium chloride is retained by cation-exchange mechanism, and triethylene glycol is retained by HILIC mechanism. Mixed-mode HILIC approach allows to retain compounds either based on multiple or single mechanisms interaction, thus providing a valuable approach for analysis. Cetylpyridinium chloride and triethylene glycol can be monitored by combination of UV and ELSD/CAD.

Condition

Column Obelisc N,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc ph 5.0
Flow Rate 1.0 ml/min
Detection UV 250nm, ELSD

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triethylene glycol, Cetylpyridinium Chloride

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Cetylpyridinium Chloride
Triethylene Glycol

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Surfactants

November 21, 2006


Surfactants are molecules that contain both hydrophilic and hydrophobic groups, usually in the form of a hydrophilic head and a hydrophobic tail. Surfactants are used in detergents where they can form micelles around hydrophobic dirt molecules and wash them away. Triton X-100 is a surfactant with a hydrophilic polyethylene oxide chain that can be separated on a Primesep D reverse-phase HPLC column based on the number of oxide units in the chain. The mobile phase is water, acetonitrile (MeCN, ACN) and sulfuric acid as buffer. UV detection at 210nm.

Condition

Column Primesep D,  4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV 210nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Triton X-100, Cetylpyridinium Chloride

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzalkonium chloride
Cetylpyridinium Chloride
Sodium
Triton X100

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Cetylpyridinium Methods with Good Efficiency and Peak Symmetry

February 11, 2004

Primesep B separates tertiary amines, such as cetylpyridinium with symmetrical peak shape by a combination of reversed-phase and ion-exclusion mechanisms. The embedded basic functional group on the stationary phase shields the underlying silanols to prevent peak tailing. Retention time can be changed by changing either organic content or acid content in the mobile phase. C18 reversed-phase columns do not typically show this tuning ability with acid content. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 250 nm.

Condition

Column Primesep B,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV 210nm

 

Description

Class of Compounds
Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Cetylpyridinium Chloride

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:

Cetylpyridinium Chloride
Pyridinium Ion
Quaternary Amines

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.