Chloramphenicol

Chloramphenicol structural formula

CAS Number 56-75-7
Molecular Formula C11H12Cl2N2O5
Molecular Weight 323.131 g/mol
InChI Key WIIZWVCIJKGZOK-RKDXNWHRSA-N
LogP 1.14
Synonyms
  • Chloramphenicol
  • 2,2-Dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide
  • Acetamide, 2,2-dichloro-N-[(1R,2R)-2-hydroxy-1-(hydroxymethyl)-2-(4-nitrophenyl)ethyl]-
  • 56-75-7
  • 4-13-00-02742
  • Acetamide, 2,2-dichloro-N-[(1R,2R)-2-hydroxy-1-(hydroxymethyl)-2-(4-nitrophenyl)ethyl]-
  • (-)-Chloramphenicol
  • [R-(R*,R*)]-2,2-Dichloro-N-[2-hydroxy-1-(hydroxymethyl)-2-(4-nitrophenyl)ethyl]acetamide
  • Acetamide, 2,2-dichloro-N-[2-hydroxy-1-(hydroxymethyl)-2-(4-nitrophenyl)ethyl]-, [R-(R*,R*)]-
  • Acetamide, 2,2-dichloro-N-[β-hydroxy-α-(hydroxymethyl)-p-nitrophenethyl]-, D-threo-(-)-
  • Ak-Chlor
  • Alficetyn
  • Amphenicol
  • Amphicol
  • Amseclor
  • Anacetin
  • Aquamycetin
  • Austracol
  • Catilan
  • Chemicetin
  • Chemicetina
  • Chlomycol
  • Chloramex
  • Chloramsaar
  • Chlorasol
  • Chloricol
  • Chlorocaps
  • Chlorocid
  • Chlorocide
  • Chlorocidin C
  • Chlorocidin C tetran
  • Chlorocin
  • Chloromycetin
  • Chloronitrin
  • Chloroptic
  • Ciplamycetin
  • Cloramfen
  • cloramfenicol
  • Cloramficin
  • Cloramicol
  • Clorocyn
  • Cloromisan
  • Cylphenicol
  • D-(-)-Chloramphenicol
  • D-(-)-threo-1-(4-Nitrophenyl)-2-dichloroacetamido-1,3-propanediol
  • D-(-)-threo-1-p-Nitrophenyl-2-dichloracetamido-1,3-propanediol
  • D-(-)-threo-1-p-Nitrophenyl-2-dichloroacetylamino-1,3-propanediol
  • D-(-)-threo-2-Dichloroacetamido-1-p-nitrophenyl-1,3-propanediol
  • D-(-)-threo-Chloramphenicol
  • D-Chloramphenicol
  • Detreomycin
  • D-threo-(1R,2R)-1-p-Nitrophenyl-2-dichloroacetamido-1,3-propanediol
  • D-threo-Chloramphenicol
  • D-threo-N-Dichloroacetyl-1-p-nitrophenyl-2-amino-1,3-propanediol
  • Embacetin
  • Enteromycetin
  • Farmicetina
  • Globenicol
  • Gloveticol
  • Halcetin
  • Intramycetin
  • Ismicetina
  • Juvamycetin
  • Kamaver
  • Kemicetine
  • Klorita
  • Klorocid S
  • Leukomyan
  • Leukomycin
  • Levocin
  • Levomicetina
  • Levomitsetin
  • Levomycetin
  • Levoplast
  • Levosin
  • Levovetin
  • Loromisin
  • Mastiphen
  • Micloretin
  • Micoclorina
  • Microcetina
  • Mychel-Vet
  • Mycinol
  • Myclocin
  • Mycochlorin
  • Normimycin V
  • Novomycetin
  • NSC 3069
  • Ocuphenicol
  • Ophthochlor
  • Pantovernil
  • Paraxin
  • Quemicetina
  • Ronphenil
  • Septicol
  • Sintomicetin
  • Sintomicetina
  • Sintomicetine R
  • Sno Phenicol
  • Soluthor
  • Stanomycetin
  • Synthomycetin
  • Tevcocin
  • Tifomycine
  • Treomicetina
  • Unimycetin
  • Veticol
  • Viceton
  • Ambofen
  • Austracil
  • Biocetin
  • Biophenicol
  • BRN 2225532
  • Chlomin
  • Chloramficin
  • Chloramfilin
  • Chloramphenicol, d-
  • Chloramphenicol crystalline
  • Chlora-tabs
  • Chloro-25 vetag
  • Chloroamphenicol
  • Chlorocid S
  • Chlorocol
  • Chloroject L
  • Chloromax
  • Chloroptic S.O.P.
  • Chlorovules
  • Cidocetine
  • Cloramidina
  • Clorosintex
  • Comycetin
  • Desphen
  • Detreomycine
  • D-(-)-2,2-Dichloro-N-(beta-hydroxy-alpha-(hydroxymethyl)-p-nitrophenylethyl)acetamide
  • Doctamicina
  • Econochlor
  • EINECS 200-287-4
  • Emetren
  • Erbaplast
  • Ertilen
  • Glorous
  • Halomycetin
  • Hortfenicol
  • Isicetin
  • Isophenicol
  • Isopto fenicol
  • Kemicetina
  • Laevomycetinum
  • Mediamycetine
  • Micochlorine
  • NCI-C55709
  • Novochlorocap
  • Novophenicol
  • Oftalent
  • Oleomycetin
  • Otachron
  • Otophen
  • Pentamycetin
  • Rivomycin
  • Romphenil
  • Sificetina
  • Sno-Phenicol
  • NSC 16331
  • Chlorofair
  • Optomycin
  • Chloramfenikol
  • Chloramphenicolum
  • Chloromycetny
  • Cloramfenicolo
  • Cloroamfenicolo
  • UNII-66974FR9Q1
  • chlornitromycin
  • laevomycetinum
  • 137731-90-9
  • 15313-32-3
  • 55172-72-0
  • 59112-59-3
  • 85666-84-8

Applications:

HPLC Separation of Common Antibiotics in Fish Farming



Fish antibiotics are used in fish farming to treat bacterial diseases of fish. It is common practice in the fish industry, particularly in developing countries, to use large amounts of antibiotics to prevent infection. The antibiotics used are often non-biodegradable and remain in the environment for long periods of time, contaminating soil and ground waters. In farming antibiotics are mixed with food and residual amount of drugs ends up in fish products and poultry, this lead to consumption of antibiotics and metabolites by humans. Five common antibiotics are separated on Primesep C column using LC/MS compatible conditions. Method can be used for quantitative determination of nitrofurantoin, nitrofurazone, furazolidone, furaltadone, chloramphenicol and nitrofurazone in fish products and environment.


Application Analytes:

5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)
Chloramphenicol
Furaltadone
Furazolidone
Nitrofurantoin
Nitrofurazone
HPLC Separation of Antibiotics in Fish Production



Five antibiotics are separated on Primesep C mixed-mode cation-exchange column using UV, ESLD and LC/MS compatible conditions. Similar compounds can be separated based on reverse phase and cation-exchange mechanisms. Current method can be used in quantitative determination of antibiotics in various food products.


Application Analytes:

5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)
Chloramphenicol
Furaltadone
Furazolidone
Nitrofurantoin
USP Methods for Chloramphenicol using a Legacy L1 Column
    Application Notes: Chloramphenicol is a common broad spectrum antibiotic developed in the 1940’s. According to the USP methods, chloramphenicol should contain no less than 97.0% and no more than 103% of chloramphenicol calculated on a dried basis. The USP HPLC method for the analysis of chloramphenicol was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Application Columns: Legacy L1 C18 HPLC column Application compounds: Chloramphenicol Mobile phase: MeOH/H2O/AcOH 45/55/0.1 Detection technique: UV Reference: USP 30: NF35

Condition
Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O/AcOH 45/55/0.1
Buffer AcOH
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description
Class of Compounds Drug,  Hydrophobic, Ionizable
Analyzing Compounds Chloramphenicol
 
Application Analytes:

Chloramphenicol
HPLC Analysis of Chloramphenicol
Chloramphenicol is a broad-spectrum antibiotic useful in treating conjunctivitis and many other infections. It is notable as an antibiotic for developing nations due to its low cost. It was analyzed on a Legacy L1 column which uses reverse-mode to retain hydrophobic compounds. Comparisons between Legacy L1 and Phenomenex columns are available upon request.

Condition
Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeOH - 45%
Buffer Acetic Acid - 0.1%
Flow Rate 1.0 ml/min
Detection UV, 280 nm

Description
Class of Compounds  Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Chloramphenicol
&
Application Analytes:

Chloramphenicol