Chlorpyrifos-methyl (Dursban)

Chlorpyrifos-methyl structural formula

CAS Number 5598-13-0
Molecular Formula C7H7Cl3NO3PS
Molecular Weight 322.520 g/mol
InChI Key HRBKVYFZANMGRE-UHFFFAOYSA-N
LogP 4.31
Synonyms
  • Chlorpyrifos-methyl
  • O,O-Dimethyl O-(3,5,6-trichloropyridin-2-yl) phosphorothioate
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridinyl) ester
  • 5598-13-0
  • Phosphorothioic acid, O,O-dimethylO-(3,5,6-trichloro-2-pyridinyl) ester
  • Chlorpyrifos M
  • Chlorpyriphos-methyl
  • clorpirifos-metil
  • Dursban ME
  • Dursban methyl
  • Methyl chlorpyrifos
  • Methyl Dursban
  • Noltran
  • O-(3,5,6-Trichloro-2-pyridinyl) O,O-dimethyl phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl) thiophosphate
  • O,O-Dimethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridinyl) ester
  • Phosphorothioic acid, O,O-dimethyl O-(3,5,6-trichloro-2-pyridyl) ester
  • BRN 1541078
  • Caswell No. 179AA
  • Chloropyriphos-methyl
  • Chlorpyrifos methyl
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridinyl) phosphorothioate
  • O,O-Dimethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate
  • O,O-Dimethyl O-(3,5,6-trichloro-2-pyridyl)phosphorothioate
  • EINECS 227-011-5
  • EPA Pesticide Chemical Code 059102
  • Methylchlorpyrifos
  • NSC 60380
  • Reldan 50 EC
  • Trichlormethylfos
  • Zertell
  • UNII-O49S38267J
  • Chlorpyrifos O,O-dimethyl analog
  • Methyl chlorpyriphos
  • O,O-dimethyl O-(3,5,6-trichloropyridin-2-yl) thiophosphate
  • Trichlormethylfos

Applications:


HPLC Analysis of Chlorpyrifos-methyl
Chlorpyrifos-methyl is an organiphosphate insecticide also known and Dursban and Lorsban. It has been banned for home use since 2001 due to its toxicity, but is one of the most widely used organophosphate insecticides according to the EPA. Chlorpyrifos-methyl is on the EURL–SRM list of target pesticides in cereals and feeding stuffs. Chlorpyrifos-methyl was retained on Obelisc R, a mixed-mode HPLC column which contains ionic groups and hydrophobic chain. Method is LC/MS compatible and can be used as a general approach for analyzing chlorpyrifos-methyl and other organophosphate insecticides.

Condition 

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl
 

Application Analytes:

Chlorpyrifos-methyl (Dursban)

Chlorpyrifos-methyl Separation Using Obelisc and Primesep Columns
Chlorpyrifos and Chlorpyrifos-methyl are amongst the most widely used pesticides. Chlorpyrifos is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EURL-CF9 and Obelisc R and Primesep B2 were used to separate chlorpyrifos-methyl and its impurities using a single multiresidue method, which can be used for many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing organophosphate pesticides.

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 4,5- 20-50 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl
 

Application Analytes:

Chlorpyrifos-methyl (Dursban)

HPLC Retention of Chlorpyrifos-methyl on Primesep 100 and Obelisc R Mixed-Mode Columns
  Chlorpyrifos-methyl, an organophosphate pesticide is considered a target pesticide by the EURL (European Union Reference Laboratory) for the EUPT-FF9 2015 a proficiency test for the analysis of a over a hundred pesticides. This compound was separated on two different mixed-mode columns, Primesep 100 and Obelisc R. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Chlorpyrifos-methyl
 

Application Analytes:

Chlorpyrifos-methyl (Dursban)

HPLC Determination of Dursban on Newcrom B Column
HPLC.cloud View on hplc.cloud HPLC Determination of Dursban on Newcrom B Column_1216

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 60/40%
Buffer AmFm pH 3.0 -  20 mM
Flow Rate 1.0 ml/min
Detection UV, 280 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Chlorpyrifos-methyl (Dursban)


Application Analytes:

Chlorpyrifos-methyl (Dursban)

HPLC Separation of 12 Pesticides on Newcrom B Column
HPLC.cloud View on hplc.cloud  

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 260 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds 6-Benzylaminopurine, Metalaxyl, Prochloraz, Paclobutrazol, Procymidone, Propanil, Chlorothalonil, Abamectin, Bifenazate, Dursban, Pentachlorophenol, Fenvalerate


Application Analytes:

6-Benzylaminopurine
Abamectin
Bifenazate
Chlorothalonil
Chlorpyrifos-methyl (Dursban)
Fenvalerate
Metalaxyl
Paclobutrazol
Pentachlorophenol
Prochloraz
Procymidone
Propanil

HPLC Separation of Pesticides on Newcrom B Column

Condition

Column Newcrom B, 4.6x150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 260, 270 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Metalaxyl, Propoxur, Paclobutrazol, Abamectin, Propanil, Iprodione,  Dursban, Pentachlorophenol, Fenvalerate


Application Analytes:

Abamectin
Chlorpyrifos-methyl (Dursban)
Fenvalerate
Iprodione
Metalaxyl
Paclobutrazol
Pentachlorophenol
Propanil
Propoxur