Chlortetracycline

CAS Number 57-62-5
Molecular Formula C22H23ClN2O8
Molecular Weight 478.881 g/mol
InChI Key CYDMQBQPVICBEU-XRNKAMNCSA-N
LogP -0.620
Synonyms
  • Chlortetracycline
  • (4S,4aS,5aS,6S,12aS)-7-Chloro-4-(dimethylamino)-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aS,5aS,6S,12aS)-
  • 57-62-5
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamin o)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pe ntahydroxy-6-methyl-1,11-dioxo-, (4S-(4.alpha.,4a. alpha.,5a.alpha.,6.beta.,12a.alpha.))-
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, (4S,4aS,5aS,6S,12aS)-
  • [4S-(4α,4aα,5aα,6β,12aα)]-7-Chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-Octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-
  • 2-Naphthacenecarboxamide, 7-chloro-4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, [4S-(4α,4aα,5aα,6β,12aα)]-
  • 7-Chlorotetracycline
  • 7-Chlortetracycline
  • Acronize
  • Acronize PD
  • Aureocarmyl
  • Aureociclin
  • Aureocina
  • Aureomicina
  • Aureomycin
  • Aureomykoin
  • Aurofac
  • Biomycin
  • Biomycin a
  • Centraureo
  • Chlortetracyclin
  • Chrusomykine
  • Chrysomykine
  • clortetraciclina
  • Duomycin
  • Flamycin
  • Orospray
  • Tri-chlortetracycline
  • Uromycin
  • Aureomycin A-377
  • Biomitsin
  • Caswell No. 219B
  • EINECS 200-341-7
  • EPA Pesticide Chemical Code 006301
  • Chlortetracyclinum
  • UNII-WCK1KIQ23Q
  • Chlormax
  • 103651-17-8
  • 1236363-43-1
  • 856305-72-1
  • 96066-24-9
  • 96968-60-4
  • 98271-27-3

Applications:


Separation of Antibiotics in Mixed-mode chromatography
  A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).  

Condition

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Buffer Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine
 

Application Analytes:

Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin

Separation of Oxytetracycline and Chlortetracycline in Mixed-Mode
Oxytetracycline and chlortetracycline are polar compounds related to broad-spectrum of tetracycline group of antibiotics. Slight hydrophobicity and presence of the basic group in both compounds allow to employ mixed-mode reversed-phase cation-exchange mechanism for separation of these compounds. Method can be used for analysis of antibiotics and related impurities with LC/MS compatible conditions.

Condition

Column Primesep 100, 4.6x150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable
Analyzing Compounds Oxytetracycline, Chlortetracycline
 

Application Analytes:

Chlortetracycline
Oxytetracycline