Creatine

Creatine structural formula

CAS Number57-00-1
Molecular FormulaC4H9N3O2
Molecular Weight131.135
InChI KeyCVSVTCORWBXHQV-UHFFFAOYSA-N
LogP-2.16
Synonyms
  • Creatine
  • N-Carbamimidoyl-N-methylglycine
  • Glycine, N-(aminoiminomethyl)-N-methyl-
  • 57-00-1
  • Glycine, N-(aminoiminomethyl)-N-methyl-
  • (1-Methylguanidino)acetic acid
  • Cosmocair C 100
  • Kre-Alkalyn
  • Methylguanidoacetic acid
  • N-amidinosarcosina
  • N-Amidinosarcosine
  • N-Amidinosarkosin
  • Neotine
  • N-Methyl-N-guanylglycine
  • NSC 8752
  • Phosphagen
  • Tego Cosmo C 100
  • Creatine, hydrate
  • EINECS 200-306-6
  • Pyrolysate
  • UNII-MU72812GK0
  • ((amino(imino)methyl)(methyl)amino)acetate
  • ((amino(imino)methyl)(methyl)amino)acetic acid
  • (N-methylcarbamimidamido)acetic acid
  • (alpha-Methylguanido)acetate
  • (alpha-methylguanido)acetic acid
  • 2-(1-methylcarbamimidamido)acetic acid
  • Creatin
  • Creatine hydrate
  • Kreatin
  • Krebiozon
  • Methylguanidoacetate
  • N-(Aminoiminomethyl)-N-Methyl-Glycine
  • N-(aminoiminomethyl)-N-methylglycine
  • [[Amino(imino)methyl](methyl)amino]acetate
  • [[Amino(imino)methyl](methyl)amino]acetic acid
  • 2-(amidino-methyl-amino)acetic acid
  • 2-(carbamimidoyl-methyl-amino)acetic acid
  • 2-(carbamimidoyl-methyl-amino)ethanoic acid
  • creatine zwitterion

Applications:

HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions

January 13, 2005

Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phormic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Condition

Column Primesep 200, 3.0×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic Acid – 0.1%
Flow Rate 0.5 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Creatine, Creatinine, Histidine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Creatine
Creatinine
Histidine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Creatine, Creatinine and Histidine

January 13, 2005

Creatine is produced by the body and plays a major role in recycling ATP. It also stabilizes pH in certain tissues. Creatine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and TFA. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 1/90%
Buffer TFA -0.1%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Creatine, Creatinine, Histidine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Creatine
Creatinine
Histidine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.