Creatine

CAS Number 57-00-1
Molecular Formula C4H9N3O2
Molecular Weight 131.135 g/mol
InChI Key CVSVTCORWBXHQV-UHFFFAOYSA-N
LogP -2.16
Synonyms
  • Creatine
  • N-Carbamimidoyl-N-methylglycine
  • Glycine, N-(aminoiminomethyl)-N-methyl-
  • 57-00-1
  • Glycine, N-(aminoiminomethyl)-N-methyl-
  • (1-Methylguanidino)acetic acid
  • Cosmocair C 100
  • Kre-Alkalyn
  • Methylguanidoacetic acid
  • N-amidinosarcosina
  • N-Amidinosarcosine
  • N-Amidinosarkosin
  • Neotine
  • N-Methyl-N-guanylglycine
  • NSC 8752
  • Phosphagen
  • Tego Cosmo C 100
  • Creatine, hydrate
  • EINECS 200-306-6
  • Pyrolysate
  • UNII-MU72812GK0
  • ((amino(imino)methyl)(methyl)amino)acetate
  • ((amino(imino)methyl)(methyl)amino)acetic acid
  • (N-methylcarbamimidamido)acetic acid
  • (alpha-Methylguanido)acetate
  • (alpha-methylguanido)acetic acid
  • 2-(1-methylcarbamimidamido)acetic acid
  • Creatin
  • Creatine hydrate
  • Kreatin
  • Krebiozon
  • Methylguanidoacetate
  • N-(Aminoiminomethyl)-N-Methyl-Glycine
  • N-(aminoiminomethyl)-N-methylglycine
  • [[Amino(imino)methyl](methyl)amino]acetate
  • [[Amino(imino)methyl](methyl)amino]acetic acid
  • 2-(amidino-methyl-amino)acetic acid
  • 2-(carbamimidoyl-methyl-amino)acetic acid
  • 2-(carbamimidoyl-methyl-amino)ethanoic acid
  • creatine zwitterion

Applications:


HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions



Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Analytes:

Creatine
Creatinine
Histidine

HPLC Separation of Creatine, Creatinine and Histidine



Creatine is produced by the body and plays a major role in recycling ATP. It also stabilizes pH in certain tissues. Creatine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Application Analytes:

Creatine
Creatinine
Histidine