Dapsone

CAS Number	80-08-0
Molecular Formula	C12H12N2O2S
Molecular Weight	248.301 g/mol
InChI Key	MQJKPEGWNLWLTK-UHFFFAOYSA-N
LogP	0.97
Synonyms	Dapsone 4,4'-Sulfonyldianiline Benzenamine, 4,4'-sulfonylbis- 80-08-0 4,4'-Sulfonyldianiline Benzenamine, 4,4'-sulfonylbis- 1,1'-Sulfonylbis[4-aminobenzene] 4,4'-Dapsone 4,4'-Diaminodiphenyl sulfone 4,4'-Diaminodiphenyl sulphone 4,4'-DIAMINODIPHENYLSULFON 4,4'-Sulfonylbisaniline 4,4'-Sulfonylbisbenzamine 4,4'-Sulfonyldiphenylamine 4,4'-Sulphonyldianiline 4-Aminophenyl sulfone Aniline, 4,4'-sulfonyldi- Aradur 976-1 Araldite HT 976 Avlosulfon Avlosulphone Bis(4-aminophenyl) sulfone Bis(p-aminophenyl) sulfone Croysulfone Croysulphone Di(4-aminophenyl)sulfone Di(p-aminophenyl) sulfone Diaphenylsulfon Diaphenylsulfone Diphenasone DIPHENYL SULFONE, 4,4'-DIAMINO- Diphone Disulone Dumitone Hardener HT 976 Metabolite C Novophone NSC 6091 p,p'-Diaminodiphenyl sulfone p,p'-Sulfonyldianiline p-Aminophenyl sulfone Seikacure S Servidapson Sulfona Sulfona-Mae Sulphadione Sumicure S Avlosulfone Bis(p-aminophenyl)sulphone Bis(4-aminophenyl)sulphone BRN 0788055 Dapsona Dapsonum Diaminodiphenyl sulfone Diamino-4,4'-diphenyl sulfone p,p-Diaminodiphenyl sulphone Diamino-4,4'-diphenyl sulphone Di(4-aminophenyl) sulfone Di(p-aminophenyl)sulphone Di(4-aminophenyl)sulphone Diaphenylsulphon Diaphenylsulphone N,N'-Diphenyl sulfondiamide DSS (VAN) Dubronax EINECS 201-248-4 NCI-C01718 NSC-6091 NSC 6091D Sulfadione Sulfone, diphenyl, 4,4'-diamino- Sulfone UCB 1,1'-Sulfonylbis(4-aminobenzene) p,p-Sulfonylbisbenzamine p,p-Sulfonylbisbenzenamine 1,1'-Sulphonylbis(4-aminobenzene) 4,4'-Sulphonylbisbenzamine 4,4'-Sulphonylbisbenzenamine Sulphonyldianiline p,p-Sulphonyldianiline Tarimyl 4,4'-Diaminodiphenyl suphone 4,4-Diaminodifenylsulfon Diaminodifenilsulfona UNII-8W5C518302 4,4'-Sulfonylbisbenzenamine DADPS DDS Dapsone p,p-sulphonylbisbenzamine p,p-sulphonylbisbenzenamine 1246638-15-2

Applications:

Separation of Antibiotics in Mixed-mode chromatography

A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).

Condition
Column	Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase	Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Buffer	Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

Description
Class of Compounds	Antibiotic, Drug, Hydrophobic, Ionizable
Analyzing Compounds	Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine

Carboxymethylcysteine
Chlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin

Analysis of Antibiotic Dapsone and Related Impurities in Mixed-Mode Chromatography

Dapsone is an antibiotic used to treat leprosy and acne. Obelisc R was used to analyze dapsone and its impurities. Obelisc R is a tri-modal reversed-phase, cation- and anion-exchange column. Method is compatible with LC/MS and prep chromatography.

Condition
Column	Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase	Gradient MeCN - 0-25%, 6 min, 25-70% 14 min
Buffer	Gradient Formic Acid - 0.05%-0.3%, 10 min, 14 min hold
Flow Rate	1.0 ml/min
Detection	UV, 270 nm

Description
Class of Compounds	Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable
Analyzing Compounds	Dapsone