Diphenamid

CAS Number 957-51-7
Molecular Formula C16H17NO
Molecular Weight 239.318 g/mol
InChI Key QAHFOPIILNICLA-UHFFFAOYSA-N
LogP 2.62
Synonyms
  • Diphenamid
  • N,N-Dimethyl-2,2-diphenylacetamide
  • Benzeneacetamide, N,N-dimethyl-alpha-phenyl-
  • 957-51-7
  • BRN 2052009
  • Caswell No. 395
  • Difenamide
  • Diherbid
  • N,N-Dimethyldiphenylacetamide
  • N,N,Dimethyl-2,2-diphenylacetamide
  • N,N-Dimethyl-alpha-phenylbenzeneacetamide
  • 2,2-Diphenyl-N,N-dimethylacetamide
  • EINECS 213-482-4
  • EPA Pesticide Chemical Code 036601
  • Lilly 34,314
  • Trefmid
  • Difenamid
  • Dimethylamid kyseliny difenyloctove
  • UNII-6Z3R6EGA2B
  • 12697-94-8
  • 73413-06-6

Applications:


HPLC Analysis of Diphenamid
 
Obelisc R was used to retain diphenamid and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing diphenamid.

Condition 

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 40%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Diphenamid
 

Application Analytes:

Diphenamid

Diphanamid Separation on Primesep and Obelisc Mixed-Mode HPLC Columns
 
Diphenamid is a pre-emergence herbicide used in production of tobacco, groundnuts, and tomatoes. Obelisc R and Primesep B2 were used to retain diphenamid and separate it from impurities. Primesep B2 contains embedded basic ion-pairing groups while Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing diphenamid and dozens of other pesticides.
 

Condition 1

Column Primesep B2, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 250 nm
   

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Diphanamid
 

Application Analytes:

Diphenamid

HPLC Analysis of Diphenamid on Primesep 100 and Obelisc R
   

Diphenamid is an herbicide which selectively controls weedy grasses and broadleaf plants in the production of tobacco, tomatoes, peppers, cotton, and many more commercial crops. Diphenamid is a preemergence herbicide that is surface applied to soils and can be expected to control weeds for 6-8 months. Diphenamid was analyzed on two different stationary phases. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.

 

Condition 1

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 25-70%
Buffer Gradient AmAc pH 3.0- 30-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Condition 3

Column Primesep 100, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-70%
Buffer Gradient AmAc pH 3.0- 20-60 mM
Flow Rate 0.4 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds  Herbicide, Insecticide, Hydrophobic, Ionizable
Analyzing Compounds Diphenamid
 

Application Analytes:

Diphenamid