DMSO (Dimethyl sulfoxide)

CAS Number	67-68-5
Molecular Formula	C2H6OS or (CH3)2SO
Molecular Weight	78.129 g/mol
InChI Key	IAZDPXIOMUYVGZ-UHFFFAOYSA-N
LogP	-0.6
Synonyms	dimethyl sulfoxide DMSO Methyl sulfoxide 67-68-5 Methylsulfinylmethane Dimethylsulfoxide Dimethyl sulphoxide Methane, sulfinylbis- Demsodrox Demasorb Demavet Dimexide Domoso Dromisol Durasorb Infiltrina Somipront Syntexan Deltan Demeso Dolicur Hyadur sulfinylbismethane Dimethyl sulfur oxide Dermasorb Dipirartril-tropico Doligur Kemsol Topsym Gamasol 90 Sclerosol Sulfinylbis(methane) Rimso-50 Dimethylsulfoxid Dimethylsulfoxyde Rimso 50 Dimethylsulphoxide Caswell No. 381 SQ 9453 Dimetil sulfoxido Sulfinylbis-methane Dimethyli sulfoxidum Topsym (rescinded) Rimso-5 NSC-763 methanesulfinylmethane Domoso (Veterinary) CCRIS 943 (methylsulfinyl)methane methylsulfoxide (CH3)2SO Dimexidum DMS-90 sulfinyldimethane NSC 763 Dimetilsolfossido [DCIT] A 10846 Dimethyl sulpoxide M 176 UNII-YOW8V9698H HSDB 80 Methane, 1,1'-sulfinylbis- EPA Pesticide Chemical Code 000177 DMS 70 DMS 90 DMS-70 Dimethylsulfoxyde [INN-French] AI3-26477 Dimetil sulfoxido [INN-Spanish] Dimethyli sulfoxidum [INN-Latin] Methyl Sulfoxide: DMSO EINECS 200-664-3 CHEMBL504 S(O)Me2 NSC763 YOW8V9698H CHEBI:28262 IAZDPXIOMUYVGZ-UHFFFAOYSA-N MFCD00002089 SQ-9453

Applications:

HPLC Separation of DMSO and Glycine by Mixed-Mode Chromatography

Dimethyl sulfoxide is important polar aprotic solvent, which is frequently used in pharmaceutical drug manufacturing, desolution, etc. DMSO is used as one of the solvents on protein chemistry due to universal ability to dissolve small molecules like amino acids. Amino acids and DMSO are very polar and have no retention on reversed-phase columns. In this HPLC application DMSO and glycine are separated on Primesep 100 mixed-mode column. DMSO is retained by weak reversed-phase mechanism and very low organic concentration is required in order to achieve any retention. Glycine, like any other underivatized amino acid, is retained by weak reversed-phase and moderate cation-exchange mechanism. Method uses acetonitrile/water/TFA gradient and UV detection.

Condition
Column	Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase	MeCN/H2O
Buffer	TFA
Flow Rate	1.0 ml/min
Detection	UV, 210 nm

Description
Class of Compounds	Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds	DMSO, Glycine

Amino Acids
DMSO (Dimethyl sulfoxide)
Glycine

HPLC Separation of Mixture of DMF and DMSO

Dimethylformamide, DMF, is primarily used as an organic solvent and is miscible with water and most other organic liquids. Dimethyl sulfoxide (DMSO) is also a solvent. It can dissolve both polar and nonpolar compounds and is miscible with water and other organic liquids. Both compounds can be retained and separated using anhydrous (water-free) conditions using HPLC by SHARC 1 column, which uses hydrogen-bonding as a separation mechanism. The method uses a gradient of acetonitrile (ACN) and methanol (MeOH) mobile phase without the need for a buffer. UV detection used at 220nm.

Condition
Column	Sharc 1, 4.6x150 mm, 5 µm, 100A
Mobile Phase	Gradient MeCN - 98-85 %, 10 min
Buffer	No
Flow Rate	1.0 ml/min
Detection	UV, 220 nm

Description
Class of Compounds	Drug, Basic, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds	DMF, DMSO

DMF (Dimethylformamide)
DMSO (Dimethyl sulfoxide)