DOPAC (Dihydroxyphenylacetic Acid)

CAS Number 102-32-9
Molecular Formula C8H8O4
Molecular Weight 168.148 g/mol
InChI Key CFFZDZCDUFSOFZ-UHFFFAOYSA-N
LogP 0.98
Synonyms
  • 3,4-Dihydroxyphenylacetic acid
  • (3,4-Dihydroxyphenyl)acetic acid
  • Benzeneacetic acid, 3,4-dihydroxy-
  • 102-32-9
  • Benzeneacetic acid, 3,4-dihydroxy-
  • (3,4-Dihydroxyphenyl)acetic acid
  • 3,4-Dihydroxybenzeneacetic acid
  • 3,4-Dihydroxyphenylessigsaure
  • Acetic acid, (3,4-dihydroxyphenyl)-
  • acide 3,4-dihydroxyphenylacetique
  • acido 3,4-dihidroxifenilacetico
  • Dihydroxyphenylacetic acid
  • Dopacetic acid
  • Homoprotocatechuic acid
  • NSC 73191
  • BRN 2211017
  • EINECS 203-024-1
  • (3,4-Dihydroxyphenyl)acetate
  • (3,4-dihydroxyphenyl)-Acetic acid
  • 2-(3,4-dihydroxyphenyl)acetic acid
  • 2-(3,4-dihydroxyphenyl)ethanoic acid
  • 3,4-DHPOP
  • 3,4-Dihydroxy-Benzeneacetic acid
  • 3,4-Dihydroxy-phenylacetic acid
  • 3,4-Dihydroxybenzeneacetate
  • 3,4-Dihydroxyphenylacetate
  • DHY
  • DOPAC
  • Dihydroxyphenylacetate
  • Dihydroxyphenylacetic acid
  • Dopacetate
  • HAA
  • Homogentisic acid

Applications:


Separation of Serotonin, Dopamine, and Related Compounds
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA
 

Application Analytes:

DOPA (3,4-dihydroxy-L-phenylalanine)
DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin

HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding

Condition

Column Sharc 1, 3.2x50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0 - 30/70%
Buffer Formic Acid - 0.5% AmFm 0.05% 7 min
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.   Application Columns: Primesep 100 Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep 100, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column
  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration. Application Columns: Primesep 100, Primesep 200 Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep 200, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid