Folic Acid

CAS Number 59-30-3
Molecular Formula C19H19N7O6
Molecular Weight 441.404 g/mol
InChI Key OVBPIULPVIDEAO-LBPRGKRZSA-N
LogP -1.77
Synonyms
  • Folic acid
  • N-(4-{[(2-Amino-4-oxo-1,4-dihydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid
  • L-Glutamic acid, N-[4-[[(2-amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-
  • 59-30-3
  • L-Glutamic acid, N-[4-[[(2-amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-
  • Acide folique
  • acido folico
  • Acifolic
  • Cytofol
  • Dosfolat B activ
  • Folacid
  • Folacin
  • Folcidin
  • Foldine
  • Folettes
  • Foliamin
  • Folicet
  • Folipac
  • Folovit
  • Folsaure
  • Folvite
  • Incafolic
  • L-GLUTAMIC ACID, N-(4-(((2-AMINO-1,4-DIHYDRO-4-OXO-6-PTERIDINYL)METHYL)AMINO)BENZOYL)-
  • L-Glutamic acid, N-[4-[[(2-amino-3,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-
  • Liver Lactobacillus casei factor
  • Millafol
  • N-[4-[[2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic acid
  • NSC 3073
  • Pteroylglutamic acid
  • Pteroyl-L-glutamic acid
  • Pteroyl-L-monoglutamic acid
  • Pteroylmonoglutamic acid
  • Vitamin Bc
  • Vitamin Be
  • Vitamin M
  • Acfol
  • N-(4-((2-Amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl)amino)benzoyl)-L-glutamic acid
  • Antianemia factor
  • EINECS 200-419-0
  • Factor U
  • Folasic
  • Folcysteine
  • Folico
  • Folina
  • L-Glutamic acid, N-(4-((2-amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl)amino)benzoyl)-
  • Glutamic acid, N-(p-(((2-amino-4-hydroxy-6-pteridinyl)methyl)amino)benzoyl)-, L-
  • Glutamic acid, N-(p-(((2-amino-4-hydroxypyrimido(4,5-b)pyrazin-6-yl)methyl)amino)benzoyl)-, L
  • Glutamic acid, pteroyl-, L-
  • Mittafol
  • PGA (VAN)
  • 4-Pteridinol, 2-amino-6-((p-((1,3-dicarboxypropyl)carbamoyl)anilino)methyl)-
  • Pteroylmonoglutamate
  • Vitamin B11
  • Acidum folicum
  • Folaemin
  • Kyselina listova
  • Nifolin
  • Novofolacid
  • UNII-935E97BOY8
  • Megafol
  • (2S)-2-[(4-{[(2-amino-4-oxo-1,4-dihydropteridin-6-yl)methyl]amino}phenyl)formamido]pentanedioic acid
  • (2S)-2-[[4-[(2-amino-4-oxo-1H-pteridin-6-yl)methylamino]benzoyl]amino]glutaric acid
  • (2S)-2-[[4-[(2-amino-4-oxo-1H-pteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
  • (2S)-2-[[4-[(2-amino-4-oxo-1H-pteridin-6-yl)methylamino]phenyl]carbonylamino]pentanedioic acid
  • (2S)-2-[[[4-[(2-amino-4-oxo-1H-pteridin-6-yl)methylamino]phenyl]-oxo-methyl]amino]pentanedioic acid
  • Folbal
  • Folipac
  • Folsaeure
  • Folsan
  • Folsav
  • N-(4-{[(2-Amino-4-oxo-3,4-dihydropteridin-6-yl)methyl]amino}benzoyl)-L-glutamic acid
  • N-[4-[[(2-Amino-3,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic acid
  • PGA
  • PteGlu
  • Pteroyl-L-glutamate
  • Pteroyl-L-monoglutamate
  • Pteroylglutamate
  • pteroyl-L-glutamic acid
  • 11096-55-2
  • 33609-88-0

Applications:


Analysis of Folic Acid and Related Impurities
Folic acid is a water-soluble vitamin. It belongs to Group B of vitamins. Folate is composed of the aromatic pteridine ring linked to para-aminobenzoic acid and one or more glutamate residues. Folic acid (Vitamin B9) and related impurities were analyzed on the Primesep 100 mixed-mode HPLC column. This column can be used for analysis of this and other water-soluble vitamins. Several impurities and folic acid are well separated. The method is robust and can be used for analysis of folic acid in supplements and other compositions.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Folic Acid
 

Application Analytes:

Folic Acid

HPLC Determination of Folic Acid onPrimesep 100 Column
HPLC Determination of Folic Acid Folic acid is a synthetic form of folate, a B vitamin, that is used as in supplements and to fortify food. Folic acid can be retained using Primesep 100 mixed-mode column, which has both hydrophobic and ion exchange properties due to embedded acidic ion-pairing groups. The analytical method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) buffer, making the method MS-compatible. Can also use UV detection at 250nm.

Condition

Column Primesep 100, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 3.0- 10 mM
Flow Rate 1.0 ml/min
Detection UV, 250 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Folic Acid
 

Application Analytes:

Folic Acid

HPLC Determination of Folic Acid (Vitamin B9) on Newcrom BH Column
Separation type: Liquid Chromatography Mixed-mode

HPLC.cloud View on hplc.cloud

Folic acid can be retained in HPLC using a Newcrom BH mixed-mode column by using a mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) as a buffer allowing the use of either UV or MS detector. Folic acid, vitamin B9, is an essential vitamin that’s used by the body to metabolise amino acids. Vitamin B-9 is a crucial prenatal vitamin. It supports cell growth, DNA synthesis, and red blood cell production. It also helps your body use iron properly.

Condition

Column Newcrom BH, 4.6x150 mm, 3 µm, 100A
Mobile Phase MeCN/H2O - 15/85%
Buffer AmFm - pH 3.0 - 20 mm
Flow Rate 1.0 ml/min
Detection UV 284 nm
 

Description

Class of Compounds Vitamin
Analyzing Compounds Folic acid, Vitamin B9
 

Application Analytes:

Folic Acid