Folic acid is a water-soluble vitamin. It belongs to Group B of vitamins. Folate is composed of the aromatic pteridine ring linked to para-aminobenzoic acid and one or more glutamate residues. Folic acid (Vitamin B9) and related impurities were analyzed on the Primesep 100 mixed-mode HPLC column. This column can be used for analysis of this and other water-soluble vitamins. Several impurities and folic acid are well separated. The method is robust and can be used for analysis of folic acid in supplements and other compositions.
HPLC Determination of Folic Acid onPrimesep 100 Column
Folic acid is a synthetic form of folate, a B vitamin, that is used as in supplements and to fortify food. Folic acid can be retained using Primesep 100 mixed-mode column, which has both hydrophobic and ion exchange properties due to embedded acidic ion-pairing groups. The analytical method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate (AmFm) buffer, making the method MS-compatible. Can also use UV detection at 250nm.