| CAS Number | 50-99-7 |
|---|---|
| Molecular Formula | C6H12O6 |
| Molecular Weight | 180.156 g/mol |
| InChI Key | WQZGKKKJIJFFOK-GASJEMHNSA-N |
| LogP | -3.24 |
| Synonyms |
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Applications:
HPLC Separation of Sugars on Primesep S2 Column
Five sugars were separated on a Primesep S2 HILIC column with LC/MS compatible conditions. Various mobile phase produce different selectivity and separation. Method can be used as alternative to aminopropyl column. Primesep S2 column is stable and does not undergo rapid hydrolysis like aminopropyl columns.
| Column | Primesep S2, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 5.0, Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds | Drug, Acid, Monocarboxylic acid, Hydrophilic, Ionizable, Hormone |
| Analyzing Compounds | Fructose, Glucose, Sucrose, Lactose, Maltose, Sorbitol, Sucrose, Alfa D-Lactose, Beta D–Lactose, Raffinose |
Application Analytes:
Fructose
Glucose
Lactose
Maltose
Raffinose
Sorbitol
Sucrose
alpha-D-Lactose
beta-D-Lactose
HPLC Separation of Mixture of D-Glucose and L-Lysine on Primesep S Column
| Column | Primesep S, 4.6x150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN - 85-60%, 10 min 2 min hold |
| Buffer | Gradient AmFm pH 3.0, 10-80 mM, 10 min 2 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Sugar, Amino Acid, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | D-Glucose, L-Lysine |
Application Analytes:
Glucose
Lysine