Glyphosate

Glyphosate structural formula

CAS Number1071-83-6
Molecular FormulaC3H8NO5P
Molecular Weight169.073
InChI KeyXDDAORKBJWWYJS-UHFFFAOYSA-N
LogP-3.40
Synonyms
  • Glyphosate
  • N-(Phosphonomethyl)glycine
  • Glycine, N-(phosphonomethyl)-
  • 1071-83-6
  • 80WDG
  • N-(phosphonomethyl) glycine
  • Glycine, N-(phosphonomethyl)-
  • (Carboxymethylamino)methylphosphonic acid
  • Carboxymethylaminomethanephosphinic acid
  • Folusen
  • Glialka
  • glyfosato
  • GlyGran
  • Glyphodin A
  • Glyphomax
  • Glyphosat
  • Glyphosate CT
  • Herbatop
  • Kickdown
  • N-(Phosphonomethyl)glycine
  • N-Phosphomethylglycine
  • N-Phosphonomethylglycine
  • NSC 151063
  • Phorsat
  • Phosphonomethylglycine
  • Phosphonomethyliminoacetic acid
  • Rebel Garden
  • Roundup Max
  • Silglif
  • BRN 2045054
  • Caswell No. 661A
  • EINECS 213-997-4
  • EPA Pesticide Chemical Code 417300
  • Pondmaster
  • UNII-4632WW1X5A
  • 37337-60-3
  • 42618-09-7
  • 75241-08-6

Applications:

HPLC Determination of Glyphosate on Newcrom B Column

March 5, 2021

Separation type: Liquid Chromatography Mixed-mode





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High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate




Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate can be retained in HPLC with a Newcrom B mixed-mode column using a mobile phase of acetonitrile (ACN, MeCN), water and phosphoric acid (H3PO4) buffer allowing the use of a UV detector at 200 nm.




 

Condition

Column Newcrom B, 3.2×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO45 – 0.08%
Flow Rate 0.5 mL/min
Detection  UV 200 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Glyphosate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Glyphosate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glufosinate, Glyphosate and Glyphosine on Newcrom B Column

December 10, 2020


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer Formic Acid Gradient
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Glufosinate, Glyphosate, Glyphosine

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Glufosinate-ammonium
Glyphosate
Glyphosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Polar Pesticides on Newcrom B Column

November 19, 2020

 

 

 

 

 

 

 

Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom B mixed-mode column.

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 60 mM 15 min
Flow Rate 1 ml/min
Detection CAD

 

Condition 2

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 15 mM 10 min
Flow Rate 1 ml/min
Detection CAD

 

 

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Aminomethylphosphonic acid (AMPA), Bialaphos, Glyphosate, Phosphorous acid H3PO3, Fosetyl-Al, Bromide, Chlorate, Perchlorate, 3-Methylphosphinicopropionic acid (MPPA), Bromide

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
3-(Methylphosphinico)propionic acid
Aminomethylphosphonic acid (AMPA)
Bialaphos
Bromate
Bromide
Chlorate
Fosetyl-Al
Glyphosate
Perchlorate
Phosphorous acid
Sodium Bromate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column

December 4, 2019


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN Gradient  -40-10%, 15 min
Buffer Formic Acid Gradient  -1- 5%, 15 min
Flow Rate 1.0 ml/min
Detection CAD

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Description

Class of Compounds Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphate Ion, Methylphosphonate, Methylenediphosphonic acid, Glyphosate, Glyphosine

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Ethylphosphonic Acid
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column

December 2, 2019

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column_1203

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 40/59%
Buffer Formic Acid – 1%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphoric Acid, Methylphosphoric Acid, Glyphosate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Ethylphosphonic Acid
Glyphosate
Methylphosphonic Acid
Phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column

December 2, 2019

Separation type: Liquid Chromatography Mixed-mode





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HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column_1202




High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate and Phosphate Ion


 
Glyphosate is a broad-spectrum herbicide which is used to kill weeds. The presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate is characterized by its carboxylic acid and phosphate group on either end.
Glyphosate and phosphate ions can be retained and separated with a Newcrom B mixed-mode column using a mobile phase consisting of acetonitrile (MeCN), water, and formic acid (H3PO4) as a buffer. This method is compatible with mass spectrometry and be detected via a Charged aerosol detector (CAD).




 

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 20%
Buffer Formic Acid – 0.5%
Flow Rate 1.0 ml/min
Detection CAD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphate, Glyphosate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Glyphosate
Phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Herbicides Paraquat, Diquat and Glyphosate in One Run with LC/MS Compatible Conditions

December 5, 2013

 

There is a need to separate different herbicides and pesticides in one run with LC/MS compatible conditions. Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)

 

Condition

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer Gradient AmFm pH 3.0 – 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Diquat
Glyphosate
Paraquat

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Paraquat, Diguat, and Glyphosate on Obelisc R Column

July 3, 2013

 

Paraquat, diquat and glyphosate are three of most widely used herbicides in the world. Paraquat and diquat are very polar and very basic quaternary amines. Glyphosate is an aminophosphonic analog of glycine. It is very polar and acidic at most of the pH of the mobile phase. Since glyphosate and the quats have opposite charges no ion-pairing method can be developed for the mixture of basic and acidic herbicides. All three herbicides were separated on the Obelisc R tri-modal column. Paraquat and diquat are retained by a cation-exchange mechanism, and glyphosate is retained by weak reversed-phase and strong anion-exchange mechanisms. This method can be used for analysis of common herbicides in fruits, vegetables, ground water, drinking water and other matrices. Method is LC/MS compatible and can be used to determine trace levels of herbicides.

 

Condition

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase MeCN – 30%
Buffer Gradient AmFm pH 3.0 – 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Chloride
Diquat
Glyphosate
Paraquat
Sodium

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea

July 3, 2013

Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Method for analysis of glyphosate in fruit juices and teas was developed using the Obelisc N HILIC/ion-exchange column. Glyphosate is retained by an ion-exchange mechanism. Since the method uses a highly aqueous mobile phase, which is not usual, for HILIC columns, none of the sugars, vitamins and other components of juices interfere with the analysis of glyphosate. Samples of juices were spiked with glyphosate. This method can be used in combination with LC/MS for determination of very low concentrations of glyphosate in various fruits and vegetables as well as ground and drinking water.

 

Condition

Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic acid – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Glyphosate

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Method for Analysis of Glyphosate in Cranberry Juice

July 3, 2013

Condition

Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic acid – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Glyphosate

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Method for Analysis of Glyphosate in Mango Juice

July 3, 2013

Application Analytes:
Glyphosate

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Method for Analysis of Glyphosate in Green Tea

July 3, 2013

 

Condition

Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic acid – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD

 

Description

Class of Compounds
Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Glyphosate

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Effect of pH on Separation of Glyphosate and Ethylphosphonic Acid

July 8, 2011

chr_295.gif

Glyphosate is broad spectrum herbicide to kill weeds. Presence of glyphosate in water, fruits and veggies is regulated. Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Method was developed for glyphosate and ethylphosphonic acid. Both compounds are retained by ion-exchange mechanism. Method is compatible with LC/MS and can be used for analysis of glyphosate and alkylphosphonic acids in various matrices.

 

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 10%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

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Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Ethylphosphonic acid

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Ethylphosphonic Acid
Glyphosate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate Reaction Intermediates and Impurities

March 3, 2010


Glyphosate and intermediates/impurities of production are separated on an Obelisc N HILIC/ion-exchange column by a combination of HILIC and ion-exchange mechanism. Method can be used in analysis of glyphosate, iminodiacetic acid, (N-phosphonomethyl)-imminodiacetic acid, diethanolamine and related impurities in reaction mixtures, waste and ground waters. Detection techniques are LC/MS, ELSD, CAD and UV.

Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Ammonium Formate
Flow Rate 1.0 ml/min
Detection ELSD

Description

Class of Compounds
Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate, IDA, PMIDA, DEA

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
DEA (Diethanolamine)
Glyphosate
IDA (Iminodiacetic acid)
PMIDA (Phosphonomethyliminodiacetic acid)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate Production Intermediates

January 13, 2010


Glyphosate is a broad spectrum herbicide used to kill weeds. It is the most used herbicide. Glyphosate is an aminophosphonic analogue of the natural amino acid glycine. Glyphosate and its intermediates are very polar ionic compounds derived from glycine. Neither of intermediates can be retained on traditional reversed-phase columns. Two methods for glyphosate intermediates were developed on Primesep B, Primesep B2 and Primesep 100 columns.

Condition

Column Primesep B, Primesep B2, Primesep 100,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, PMIDAA, IDA

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
DEA (Diethanolamine)
Diethanolamine
Glyphosate
IDA (Iminodiacetic acid)
Iminodiacetic Acid
PMIDA (Phosphonomethyliminodiacetic acid)
Phosphonomethyliminodiacetic Acid

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Isopropylamine and Glyphosate

December 6, 2007

Condition

Column Primesep B,  4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Isppropylamine

 

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Glyphosate
Isopropylamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Glyphosate by Cation Exchange and Anion Exchange

July 6, 2003

Primesep 100 separates a mixture of glyphosate and isopropylamine by HPLC using cation exchange as a retention mechanism. The peak order of this mixture is reversed on a Primesep B column which uses anion exchange. This combination offers an alternative to ion chromatography for the simultaneous analysis of complex herbicide mixtures. Glyphosate, other phosphonomethyl herbicides, and their amine salts can be analyzed with a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and evaporative light scattering detection (ELSD).

Condition

Column Primesep B, Primesep 100, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 0.5 ml/min
Detection ELSD

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Isppropylamine

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Glyphosate
Isopropylamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.