Glyphosate

CAS Number 1071-83-6
Molecular Formula C3H8NO5P
Molecular Weight 169.073 g/mol
InChI Key XDDAORKBJWWYJS-UHFFFAOYSA-N
LogP -3.40
Synonyms
  • Glyphosate
  • N-(Phosphonomethyl)glycine
  • Glycine, N-(phosphonomethyl)-
  • 1071-83-6
  • 80WDG
  • N-(phosphonomethyl) glycine
  • Glycine, N-(phosphonomethyl)-
  • (Carboxymethylamino)methylphosphonic acid
  • Carboxymethylaminomethanephosphinic acid
  • Folusen
  • Glialka
  • glyfosato
  • GlyGran
  • Glyphodin A
  • Glyphomax
  • Glyphosat
  • Glyphosate CT
  • Herbatop
  • Kickdown
  • N-(Phosphonomethyl)glycine
  • N-Phosphomethylglycine
  • N-Phosphonomethylglycine
  • NSC 151063
  • Phorsat
  • Phosphonomethylglycine
  • Phosphonomethyliminoacetic acid
  • Rebel Garden
  • Roundup Max
  • Silglif
  • BRN 2045054
  • Caswell No. 661A
  • EINECS 213-997-4
  • EPA Pesticide Chemical Code 417300
  • Pondmaster
  • UNII-4632WW1X5A
  • 37337-60-3
  • 42618-09-7
  • 75241-08-6

Applications:


Separation of Glyphosate by Cation Exchange and Anion Exchange



Primesep 100 separates a mixture of glyphosate and isopropylamine by HPLC using cation exchange as a retention mechanism. The peak order of this mixture is reversed on a Primesep B column which uses anion exchange. This combination offers an alternative to ion chromatography for the simultaneous analysis of complex herbicide mixtures. Glyphosate, other phosphonomethyl herbicides, and their amine salts can be analyzed with a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and evaporative light scattering detection (ELSD).



Application Analytes:

Glyphosate
Isopropylamine

HPLC Separation of Glyphosate Production Intermediates


Glyphosate is a broad spectrum herbicide used to kill weeds. It is the most used herbicide. Glyphosate is an aminophosphonic analogue of the natural amino acid glycine. Glyphosate and its intermediates are very polar ionic compounds derived from glycine. Neither of intermediates can be retained on traditional reversed-phase columns. Two methods for glyphosate intermediates were developed on Primesep B, Primesep B2 and Primesep 100 columns.



Application Analytes:

DEA (Diethanolamine)
Diethanolamine
Glyphosate
IDA (Iminodiacetic acid)
Iminodiacetic Acid
PMIDA (Phosphonomethyliminodiacetic acid)
Phosphonomethyliminodiacetic Acid

HPLC Separation of Isopropylamine and Glyphosate





Application Analytes:

Glyphosate
Isopropylamine

HPLC Separation of Glyphosate Reaction Intermediates and Impurities


Glyphosate and intermediates/impurities of production are separated on an Obelisc N HILIC/ion-exchange column by a combination of HILIC and ion-exchange mechanism. Method can be used in analysis of glyphosate, iminodiacetic acid, (N-phosphonomethyl)-imminodiacetic acid, diethanolamine and related impurities in reaction mixtures, waste and ground waters. Detection techniques are LC/MS, ELSD, CAD and UV.



Application Analytes:

DEA (Diethanolamine)
Glyphosate
IDA (Iminodiacetic acid)
PMIDA (Phosphonomethyliminodiacetic acid)

Effect of pH on Separation of Glyphosate and Ethylphosphonic Acid
chr_295.gif Glyphosate is broad spectrum herbicide to kill weeds. Presence of glyphosate in water, fruits and veggies is regulated. Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Method was developed for glyphosate and ethylphosphonic acid. Both compounds are retained by ion-exchange mechanism. Method is compatible with LC/MS and can be used for analysis of glyphosate and alkylphosphonic acids in various matrices.  

Condition

Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 10%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C
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Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Ethylphosphonic acid


Application Analytes:

Ethylphosphonic Acid
Glyphosate

Separation of Paraquat, Diguat, and Glyphosate on Obelisc R Column
 

Paraquat, diquat and glyphosate are three of most widely used herbicides in the world. Paraquat and diquat are very polar and very basic quaternary amines. Glyphosate is an aminophosphonic analog of glycine. It is very polar and acidic at most of the pH of the mobile phase. Since glyphosate and the quats have opposite charges no ion-pairing method can be developed for the mixture of basic and acidic herbicides. All three herbicides were separated on the Obelisc R tri-modal column. Paraquat and diquat are retained by a cation-exchange mechanism, and glyphosate is retained by weak reversed-phase and strong anion-exchange mechanisms. This method can be used for analysis of common herbicides in fruits, vegetables, ground water, drinking water and other matrices. Method is LC/MS compatible and can be used to determine trace levels of herbicides.

 

Condition

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer Gradient AmFm pH 3.0 - 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate


Application Analytes:

Chloride
Diquat
Glyphosate
Paraquat
Sodium

Method for Analysis of Glyphosate in Apple Juice, Cranberry Juice, Mango Juice and Green Tea
Glyphosate is a broad-spectrum herbicide which is used to kill weeds. Presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Method for analysis of glyphosate in fruit juices and teas was developed using the Obelisc N HILIC/ion-exchange column. Glyphosate is retained by an ion-exchange mechanism. Since the method uses a highly aqueous mobile phase, which is not usual, for HILIC columns, none of the sugars, vitamins and other components of juices interfere with the analysis of glyphosate. Samples of juices were spiked with glyphosate. This method can be used in combination with LC/MS for determination of very low concentrations of glyphosate in various fruits and vegetables as well as ground and drinking water.  

Condition

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic acid - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD
 

Description

Class of Compounds Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate
 

Application Analytes:

Glyphosate

Method for Analysis of Glyphosate in Cranberry Juice

Condition

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic acid - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD
 

Description

Class of Compounds Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate
 

Application Analytes:

Glyphosate

Method for Analysis of Glyphosate in Mango Juice



Application Analytes:

Glyphosate

Method for Analysis of Glyphosate in Green Tea
 

Condition

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer Formic acid - 0.05%
Flow Rate 1.0 ml/min
Detection UV, 250 nm, ELSD
 

Description

Class of Compounds Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Glyphosate
 

Application Analytes:

Glyphosate

Separation of Herbicides Paraquat, Diquat and Glyphosate in One Run with LC/MS Compatible Conditions
 

There is a need to separate different herbicides and pesticides in one run with LC/MS compatible conditions. Paraquat, diquat and glyphosate were separated on reversed-phase tri-modal cation- and anion-exchange column (Obelisc R) and on HILIC/ion-exchange columns (Obelisc N). Method explores unique properties of mixed-mode stationary phase which retains and separates cations like paraquat and diquat and anions/zwitter-ions like glyphosate in one run. Since columns are compatible with 100% organic and 100% water, a wide range of gradients can be used for analysis as well as isocratic conditions where it is desired. Method can be used for quantitation of these compounds in various matrices (soil, ground water, crops, food, etc.)

 

Condition

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase MeCN - 30%
Buffer Gradient AmFm pH 3.0 - 5-30 mM, 10 min
Flow Rate 0.4 ml/min
Detection UV, 250 nm, ELSD
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Paraquat, Diquat, Glyphosate


Application Analytes:

Diquat
Glyphosate
Paraquat

HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column
HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column_1202    

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 20%
Buffer Formic Acid - 0.5%
Flow Rate 1.0 ml/min
Detection CAD
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphate, Glyphosate


Application Analytes:

Glyphosate
Phosphate

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column
HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column_1203

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 40/59%
Buffer Formic Acid - 1%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphoric Acid, Methylphosphoric Acid, Glyphosate


Application Analytes:

Ethylphosphonic Acid
Glyphosate
Methylphosphonic Acid
Phosphate

HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
 

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN Gradient  -40-10%, 15 min
Buffer Formic Acid Gradient  -1- 5%, 15 min
Flow Rate 1.0 ml/min
Detection CAD
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Description

Class of Compounds Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic acid,                      Phosphate Ion, Methylphosphonate, Methylenediphosphonic acid, Glyphosate, Glyphosine


Application Analytes:

Ethylphosphonic Acid
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate