CAS Number 113-00-8
Molecular Formula CH5N3
Molecular Weight 59.072 g/mol
LogP -1.45
  • Guanidine
  • 113-00-8
  • 4-03-00-00148
  • Aminoformamidine
  • Aminomethanamidine
  • Carbamamidine
  • Carbamidine
  • Guanidin
  • guanidina
  • Imidourea
  • Iminourea
  • BRN 0506044
  • EINECS 204-021-8
  • 113978-92-0
  • 113978-93-1
  • 117365-55-6
  • 152252-01-2
  • 475470-57-6
  • 756878-05-4


Separation of Guanidine on Primesep 100 Column
Primesep 100 separates guanidine from its sodium counterion on a short 50 mm column by cation exchange. Guanidine is found in urine as a product of protein metabolism and is also used in the manufacture of plastics and explosives. Guanidine is detected by UV detection at 200 nm, but UV cannot detect the sodium ion. If an evaporative light scattering detector (ELSD) is used, both components can be detected. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA).


Column Primesep 100, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection ELSD, UV 200nm


Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Guanidine

Application Analytes:


HPLC Application for Retention of Guanidine by Mixed-Mode Chromatography

Guanidine is highly basic (pKa 12.5), and a polar compound is formed by the oxidation of guanine. In the human body, guanine is a byproduct of protein metabolism and can be found in urine. Guanine can be retained by the cation-exchange mechanism on Primesep 100 column. Guanine is slightly UV active and can be observed at 200 nm. A better detection technique is ELSD, which allows you to see low concentrations of guanine. The temperature of the ELSD must be maintained relatively low to insure proper sensitivity of the method. This method can be used for analysis of other guanidine-based compounds. If substituted guanidine has hydrophobic properties, it will retain based on ion-exchange and reverse phase mechanisms.

Application Analytes:


HPLC Separation of Guanidine and Benzoic Acids on Primesep 100 Column

Application Analytes:

Benzoic Acid
Dinitrophenylacetic Acid