Guanine

CAS Number 73-40-5
Molecular Formula C5H5N5O
Molecular Weight 151.129 g/mol
InChI Key UYTPUPDQBNUYGX-UHFFFAOYSA-N
LogP -0.910
Synonyms
  • Guanine
  • 2-Amino-1,9-dihydro-6H-purin-6-one
  • 6H-Purin-6-one, 2-amino-1,9-dihydro-
  • 73-40-5
  • 6H-Purin-6-one, 2-amino-1,7-dihydro-
  • 2-Amino-1,7-dihydro-6H-purin-6-one
  • 2-Amino-6-hydroxy-1H-purine
  • 2-Amino-6-hydroxypurine
  • 2-Aminohypoxanthine
  • 6H-Purin-6-one, 2-amino-1,9-dihydro-
  • C.I. Natural White 1
  • Dew Pearl
  • guanina
  • Guanine enol
  • Hypoxanthine, 2-amino-
  • Mearlmaid
  • Mearlmaid AA
  • Natural Pearl Essence
  • Natural White 1
  • Pearl Essence
  • Stella Polaris
  • EINECS 200-799-8
  • UNII-5Z93L87A1R
  • 2-Amino-1,9-dihydro-6H-purin-6-one
  • 2-Amino-1,9-dihydro-purin-6-one
  • 2-Amino-3,7-dihydro-6H-purin-6-one
  • 2-Amino-6-purinol
  • 2-Amino-Hypoxanthine
  • 2-amino-6,7-dihydro-3H-purin-6-one
  • 2-amino-6-oxopurine
  • 6-Hydroxy-2-aminopurine
  • CI Natural white 1
  • GUN
  • Gua
  • Guanin
  • Mearlmaid
  • Naturon
  • Pathocidin
  • 11006-44-3
  • 158475-99-1
  • 15986-36-4
  • 37432-34-1
  • 492-33-1
  • 54435-87-9
  • 66224-64-4
  • 69257-39-2
  • 8039-79-0
  • 929247-29-0
  • 934585-79-2
  • 936902-27-1

Applications:


Separation of Nucleic Bases


Primesep 200 separates with baseline resolution nucleic bases (uracil, thymine, cytosine, guanine, and adenine) by a combination of cation exchange and reversed phase. Uracil typically does not retain on reversed-phase column and is often used as an unretained void volume marker for C18 and C8 columns. Primesep 200 has an embedded anionic functional group which helps retain polar compounds polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Application Analytes:

Cytosine
Guanine
Nucleic Bases
Thymine
Uracil

HPLC Application for Separation of Nucleotide Bases
Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line separation on traditional C18 as peaks of nucleotide bases co-elute even at low organic concentration. In this application nucleobases are well retained and separated on Primesep 200 mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. Method can utilize UV, ELSD, and LC/MS detection.

Application Analytes:

Adenine
Cytosine
Guanine
Purines
Pyrimidines
Uracil