Guanine

Guanine structural formula

CAS Number73-40-5
Molecular FormulaC5H5N5O
Molecular Weight151.129
InChI KeyUYTPUPDQBNUYGX-UHFFFAOYSA-N
LogP-0.910
Synonyms
  • Guanine
  • 2-Amino-1,9-dihydro-6H-purin-6-one
  • 6H-Purin-6-one, 2-amino-1,9-dihydro-
  • 73-40-5
  • 6H-Purin-6-one, 2-amino-1,7-dihydro-
  • 2-Amino-1,7-dihydro-6H-purin-6-one
  • 2-Amino-6-hydroxy-1H-purine
  • 2-Amino-6-hydroxypurine
  • 2-Aminohypoxanthine
  • 6H-Purin-6-one, 2-amino-1,9-dihydro-
  • C.I. Natural White 1
  • Dew Pearl
  • guanina
  • Guanine enol
  • Hypoxanthine, 2-amino-
  • Mearlmaid
  • Mearlmaid AA
  • Natural Pearl Essence
  • Natural White 1
  • Pearl Essence
  • Stella Polaris
  • EINECS 200-799-8
  • UNII-5Z93L87A1R
  • 2-Amino-1,9-dihydro-6H-purin-6-one
  • 2-Amino-1,9-dihydro-purin-6-one
  • 2-Amino-3,7-dihydro-6H-purin-6-one
  • 2-Amino-6-purinol
  • 2-Amino-Hypoxanthine
  • 2-amino-6,7-dihydro-3H-purin-6-one
  • 2-amino-6-oxopurine
  • 6-Hydroxy-2-aminopurine
  • CI Natural white 1
  • GUN
  • Gua
  • Guanin
  • Mearlmaid
  • Naturon
  • Pathocidin
  • 11006-44-3
  • 158475-99-1
  • 15986-36-4
  • 37432-34-1
  • 492-33-1
  • 54435-87-9
  • 66224-64-4
  • 69257-39-2
  • 8039-79-0
  • 929247-29-0
  • 934585-79-2
  • 936902-27-1

Applications:

HPLC Separation of Uracil, Thymine, Hypoxanthine and Guanine on Newcrom AH

October 31, 2023

HPLC Method for Analysis of Uracil, Thymine, Hypoxanthine, Guanine on Newcrom AH Column by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analyses of Uracil, Thymine, Hypoxanthine and Guanine

Uracil, Thymine, Hypoxanthine, and Guanine are all nitrogenous bases, each playing distinct roles in the biochemistry of nucleic acids.

Uracil (U):

  • Found in: RNA
  • Pairs with: Adenine (A)
  • Structure: Pyrimidine
  • Role: Uracil replaces thymine in RNA. In DNA, adenine pairs with thymine, but in RNA, adenine pairs with uracil.

Thymine (T):

  • Found in: DNA
  • Pairs with: Adenine (A)
  • Structure: Pyrimidine
  • Role: Thymine is specific to DNA, distinguishing it from RNA. It’s the base that pairs with adenine through two hydrogen bonds.

Hypoxanthine:

  • A naturally occurring purine derivative. It’s not directly a base in standard DNA or RNA, but it plays a significant role in the metabolism of purines.
  • It is the base form of the nucleoside inosine, which can be found in certain tRNAs and plays a role in wobble base pairing.
  • Hypoxanthine is also an intermediate in the purine degradation pathway, leading to the production of uric acid.

Guanine (G):

  • Found in: Both DNA and RNA
  • Pairs with: Cytosine (C)
  • Structure: Purine
  • Role: Guanine is one of the four main nucleobases in the nucleic acids DNA and RNA. It forms three hydrogen bonds with cytosine, contributing to the stability of the nucleic acid structures.

These nitrogenous bases are crucial for the structure, replication, and function of nucleic acids. Understanding their properties and interactions is fundamental to molecular biology and genetics.

Nucleotides can be retained and analyzed on a mixed-mode Newcrom AH column with a mobile phase consisting of water, Acetonitrile (MeCN), and ammonium formate. This analytical method can detect compounds with high resolution and peak symmetry using UV detection at 260 nm

High Performance Liquid Chromatography (HPLC) Method for Analyses of Uracil, Thymine, Hypoxanthine and Guanine

Condition

ColumnNewcrom AH, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 1%
Buffer Ammonium formate pH 3.0- 6 mM
Flow Rate1.0 ml/min
Peak Retention Time2.21, 2.38, 2.81, 7.92 min
Detection260 nm

Description

Class of CompoundsNucleotides
Analyzing CompoundsUracil, Thymine, Hypoxanthine, Guanine

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Guanine
Hypoxanthine
Thymine
Uracil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+

December 5, 2022

Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Deoxguanosine, Guanine and Guanosine on BIST B+
Ionic Modifier Effect on Selectivity of Separation of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine


Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN
BufferH3PO4, H2SO4, HFGA (Hexafluoroglutaric acid) – 0.2%,
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time

Description

Class of CompoundsNucleosides
Analyzing CompoundsDeoxyguanosine, Guanine and Guanosine

Application Column

BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Deoxyguanosine
Guanine
Guanosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+

December 5, 2022

HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Deoxguanosine, Guanine and Guanosine on BIST B+
HPLC Method for Analysis of Deoxyguanosine, Guanine and Guanosine on BIST B+ by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Deoxyguanosine, Guanine and Guanosine


Condition

ColumnBIST B+, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN – 85%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 260 nm
Peak Retention Time9.6, 11.2, 12.8 min

Description

Class of CompoundsNucleosides
Analyzing CompoundsDeoxyguanosine, Guanine and Guanosine

Application Column

BIST B+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Deoxyguanosine
Guanine
Guanosine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH

April 14, 2020
HPLC Separation of Uracil, Thymine, Guanine, Cytosine, Adenine on Newcrom AH Column_1245

Uracil, Thymine, Guanine, Cytosine and Adenine are the nucleobases found in RNA and DNA.  The nucleobases are difficult to separate on reverse-phase columns due to their polar, hydrophilic and ionic nature.  Using the Newcrom AH mixed-mode column, the nucleobases can be easily separated isocratically using low organic mobile phase (5% acetonitrile) or pure water, if organic mobile phase is undesirable, with ammonium formate buffer, making the method both UV and Mass Spec compatible.

ColumnNewcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 5/95%
BufferAmFm pH 3.0- 30 mM
Flow Rate1.0 ml/min
DetectionUV 260 nm,  MS-compatible mobile phase

ColumnNewcrom AH, 4.6×150 mm, 5 µm, 100A
Mobile PhaseH2O – 100%
BufferAmFm pH 3.0- 10 mM
Flow Rate1.0 ml/min
DetectionUV 260 nm,  MS-compatible mobile phase

Class of CompoundsHydrophilic, Drug, Xanthine, Nucleic Bases
Analyzing CompoundsUracil, Thymine, Guanine, Cytosine, Adenine

Application Column

Newcrom AH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Adenine
Cytosine
Guanine
Thymine
Uracil
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Application for Separation of Nucleotide Bases Uracil, Thymine, Guanine, Cytosine, Adenine on Primesep 200 Column

December 6, 2007

Nucleotide bases are parts of DNA and RNA. Adenine and guanine are purine-bases; uracil, thymine and cytosine are pyrimidine-bases. In the view of chromatography these compounds are very polar and similar in properties. It is hard to obtain base line HPLC separation on traditional C18 as peaks of nucleotide bases co-elute even at low organic concentration. In this application nucleobases are well retained and separated on Primesep 200 mixed-mode column. Compounds are retained by weak reverse phase and weak ion-exchange mechanisms. This HPLC method can utilize UV, ELSD, and LC/MS detection.

Condition

Column Primesep 200, 4.6*250 mm,  5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer TFA – 0.2%
Flow Rate 0.5 ml/min, 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Thymine, Cytosine, Guanine, Adenine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Adenine
Cytosine
Guanine
Purines
Pyrimidines
Uracil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Nucleic Bases

September 24, 2003

Primesep 200 separates with baseline resolution nucleic bases (uracil, thymine, cytosine, guanine, and adenine) by a combination of cation exchange and reversed phase. Uracil typically does not retain on reversed-phase column and is often used as an unretained void volume marker for C18 and C8 columns. Primesep 200 has an embedded anionic functional group which helps retain polar compounds polar and ion-exchange mechanisms. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) with UV detection at 270 nm.

Condition

Column Primesep 200, 4.6*250 mm,  5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer TFA – 0.2%
Flow Rate 0.5 ml/min, 1.0 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Thymine, Cytosine, Guanine, Adenine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Adenine
Cytosine
Guanine
Nucleic Bases
Thymine
Uracil

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.