HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid) structural formula

CAS Number 7365-45-9
Molecular Formula C8H18N2O4S
Molecular Weight 238.301 g/mol
InChI Key JKMHFZQWWAIEOD-UHFFFAOYSA-N
LogP -2.26
Synonyms
  • (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazin-4-ium-1-yl]ethane-1-sulfonate
  • 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
  • 7365-45-9
  • HEPES
  • 5-23-02-00379
  • 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
  • 1-[4-(2-Hydroxyethyl)-1-piperazinyl]ethane-2-sulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
  • 2-[4-(2-Hydroxyethyl)piperazinyl]ethanesulfonic acid
  • 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
  • 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonic acid
  • 4-(2-Hydroxyethyl)piperazin-1-ylethansulfonsaure
  • Acide 4-(2-hydroxyethyl)piperazin-1-ylethanesulfonique
  • acide 4-(2-hydroxyethyl)piperazine-1-ylethanesulfonique
  • acido 4-(2-hidroxietil)piperazin-1-iletanosulfonico
  • N-(2-Hydroxyethyl)piperazine-N'-2'-ethanesulfonic acid
  • N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid
  • N-(2-Hydroxyethyl)piperazine-N'-ethanesulfonic acid
  • N'-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
  • N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
  • NSC 166663
  • PIPERAZINE, 1-(ETHYLSULFONIC ACID)-4- (2-HYDROXYETHYL)-
  • BRN 0883043
  • EINECS 230-907-9
  • N-2-Hydroxyethylpiperazine N',2'-ethanesulfonic acid
  • N-2-Hydroxyethylpiperazine-N-ethane sulfonic acid
  • 4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acid
  • N-2-Hydroxyethylpiperazine-N'-ethanesulfonate
  • N-2-Hydroxyethylpiperazine-N'-ethanesulfonic acid
  • UNII-RWW266YE9I
  • 2-[4-(2-hydroxyethyl)-2,3,5,6-tetrahydropyrazin-1-yl]ethanesulfonate
  • 2-[4-(2-hydroxyethyl)piperazin-4-ium-1-yl]ethanesulfonate

Applications:


Effect of Buffer on HPLC Separation of Buffers



HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.



Application Analytes:

2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)
CAPS (3-(Cyclohexylamino)propanesulfonic acid)
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)
Zwitterion

HPLC Determination of HEPES on Newcrom AH Column
HPLC.cloud View on hplc.cloud

Condition

Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase H2O- 100%
Buffer Methanesulfonic acid  - 10 mM
Flow Rate 1.0 ml/min
Detection Conductivity
 

Description

Class of Compounds Hydrophilic
Analyzing Compounds HEPES
 

Application Analytes:

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)