Histidine | SIELC

CAS Number	71-00-1
Molecular Formula	C6H9N3O2
Molecular Weight	155.157 g/mol
InChI Key	HNDVDQJCIGZPNO-YFKPBYRVSA-N
LogP	-3.32
Synonyms	Histidine L-Histidine 71-00-1 Histidine, L- 8: PN: EP2878667 TABLE: 5 claimed sequence (2S)-2-Amino-3-(1H-imidazol-5-yl)propanoic acid L-Histidine (2S)-2-Amino-3-(1H-imidazol-4-yl)propanoic acid (S)-4-(2-Amino-2-carboxyethyl)imidazole (S)-Histidine (S)-α-Amino-1H-imidazole-4-propanoic acid 1H-Imidazole-4-alanine, (S)- 1H-Imidazole-4-propanoic acid, α-amino-, (S)- Glyoxaline-5-alanine Histidin histidina HISTIDINE, L- L-(-)-Histidine L-Alanine, 3-(1H-imidazol-4-yl)- L-Hystidine NSC 137773 4-(2-Amino-2-carboxyethyl)imidazole alpha-Amino-1H-imidazole-4-propionic acid, (S)- alpha-Amino-4(or 5)-imidazolepropionic acid Anti-rheuma EINECS 200-745-3 FEMA No. 3694 S-Histidine 1H-Imidazole-4-propanoic acid, alpha-amino-, (S)- Istidina L-beta-(4-Imidazolyl)alanin L-beta-(4-Imidazolyl)-alpha-alanin Histidinum UNII-4QD397987E (2S)-2-amino-3-(3H-imidazol-4-yl)propanoic acid (S)-1H-Imidazole-4-alanine (S)-2-Amino-3-(4-imidazolyl)propionsaeure (S)-a-Amino-1H-imidazole-4-propanoate (S)-a-Amino-1H-imidazole-4-propanoic acid (S)-alpha-Amino-1H-imidazole-4-propanoate (S)-alpha-Amino-1H-imidazole-4-propionate (S)-alpha-amino-1H-Imidazole-4-propanoic acid (S)1H-Imidazole-4-alanine 3-(1H-Imidazol-4-yl)-L-Alanine Amino-1H-imidazole-4-propanoate Amino-1H-imidazole-4-propanoic acid Amino-4-imidazoleproprionate Amino-4-imidazoleproprionic acid Glyoxaline-5-alanine L-Histidin (2S)-2-ammonio-3-(1H-imidazol-4-yl)propanoate L-histidine zwitterion 150-35-6 155304-24-8 35479-49-3 35558-59-9 45955-20-2 54166-13-1 7006-35-1 736075-03-9

Applications:

Amino Acids Analysis in Acid Gradient Condition

11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

HPLC Separation of Creatine, Creatinine and Histidine with MS Conditions

Creatine, creatinine, and histidine are all important amino acids in the human body. All of these can be separated by liquid chromatography. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Creatine
Creatinine
Histidine

HPLC Separation of Creatine, Creatinine and Histidine

Creatine is produced by the body and plays a major role in recycling ATP. It also stabilizes pH in certain tissues. Creatine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It also suitable for pharmacokinetics.

Creatine
Creatinine
Histidine

HPLC Analysis of Polysorbate in Mixture with Amino Acids and Sugar

Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are often used in cosmetics to solubilize essential oils into water-based products. Polysorbates are oily liquids derived from PEG-ylated sorbitan (a derivative of sorbitol) esterified with fatty acids. Surfactants that are esters of plain (non-PEG-ylated) sorbitan with fatty acids are usually referred to by the name Span. It is often required to quantitate Polysorbate (Polysorbate/Tween 20, Polysorbate/Tween 40, Polysorbate 60/Tween 60 and Polysorbate 80) by HPLC in various formulations. Polysorbates exist in form of oligomers.

Amino Acids
Glycine
Histidine
Polysorbate
Polysorbate 20
Polysorbate 80
Sucrose
Tween 20
Tween 80

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column

Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.

Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine

HPLC Separation of Mixture of Essential Amino Acids

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
Methionine
Phenylalanine
Tryptophan
Valine

HPLC Separation of Mixture of 12 Amino Acids

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
Methionine
Phenylalanine
Proline
Tryptophan
Valine