Homovanillic Acid

CAS Number 306-08-1
Molecular Formula C9H10O4
Molecular Weight 182.176 g/mol
InChI Key QRMZSPFSDQBLIX-UHFFFAOYSA-N
LogP 0.33
Synonyms
  • Homovanillic acid
  • (4-Hydroxy-3-methoxyphenyl)acetic acid
  • Benzeneacetic acid, 4-hydroxy-3-methoxy-
  • 306-08-1
  • Benzeneacetic acid, 4-hydroxy-3-methoxy-
  • (4-Hydroxy-3-methoxyphenyl)acetic acid
  • 3-Methoxy-4-hydroxyphenylacetic acid
  • 4-Hydroxy-3-methoxybenzeneacetic acid
  • 4-hydroxy-3-methoxyphenylacetic acid
  • 4-Hydroxy-3-methoxyphenylessigsaure
  • Acetic acid, (4-hydroxy-3-methoxyphenyl)-
  • Acide 4-hydroxy-3-methoxyphenylacetique
  • acido 4-hidroxi-3-metoxifenilacetico
  • NSC 16682
  • Vanilacetic acid
  • EINECS 206-176-7
  • UNII-X77S6GMS36
  • (4-Hydroxy-3-methoxyphenyl)acetate
  • 2-(4-hydroxy-3-methoxy-phenyl)acetic acid
  • 2-(4-hydroxy-3-methoxy-phenyl)ethanoic acid
  • 3-Methoxy-4-hydroxy-phenylacetic acid
  • 3-Methoxy-4-hydroxyphenylacetate
  • 3-Methoxy-4-hydroxyphenylacetic acid
  • 4-Hydroxy-3-methoxybenzeneacetate
  • 4-hydroxy 3-methoxyphenylacetic acid
  • HVA
  • Homovanilate
  • Homovanilic acid
  • Homovanillate
  • Homovanillinic acid
  • Vanilacetate
  • Vanillacetic acid

Applications:


HPLC Separation of Vanilmandelic, Hydroxyindoleacetic, and Homovanillic Acids

Vanilmandelic, hydroxyindoleacetic and homovanillic acids are metabolites of various neurotransmitters that are used in urine tests to measure the levels of those neurotransmitters in the body. They can be baseline separated on a reverse-phase Primesep D HPLC column by varying the buffers and pH of the mobile phase consisting of water and acetonitrile (MeCN, ACN). UV detection at 230nm.

Application Analytes:

5-Hydroxyindole-3-Acetic Acid
Homovanillic Acid
Vanilmandelic Acid

Separation of Serotonin, Dopamine, and Related Compounds
Catecholamines are chemical compounds derived from the amino acid tyrosine containing catechol and amine groups. Some of them are biogenic amines. Retention of compounds of the catecholamine pathway is achieved on Obelisc N column. All polar compounds are well retained by combination of HILIC and ion-exchange mechanisms. Obelisc N columns produce very good peak shapes for all analytes. The method is very sensitive to amount of ACN, buffer and buffer pH. The retention time changes with variation of the main parameters. This method can be used for quantitation of biogenic amines and related compounds (homovanillic acid, dihydroxyphenyl acetic acid, serotonin, dopamine, epinephrine, hydroxytryptophan, epinephrine and DOPA) in urine, blood and other biological fluids. Further optimization of this HPLC method can be used during screening and validation. Amines and acids can be analyzed in the same run and retained by a combination of polar organic mode, cation-exchange and anion-exchange modes. Various buffers within specified pH can be employed (ammonium formate, ammonium acetate, sodium phosphate, etc.).

Condition

Column Obelisc N, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds Homovanillic acid, Dihydroxyphenyl acetic acid, Serotonin, Dopamine, Epinephrine, Hydroxytryptophan, DOPA
 

Application Analytes:

DOPA (3,4-dihydroxy-L-phenylalanine)
DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Epinephrine
Homovanillic Acid
Hydroxytryptophan
Norepinephrine
Serotonin

HPLC Separation of Homovanillic Acid, Dopamine, and DOPAC using Hydrogen Bonding

Condition

Column Sharc 1, 3.2x50 mm, 5 µm, 100A
Mobile Phase Gradient MeCN/MeOH - 100/0 - 30/70%
Buffer Formic Acid - 0.5% AmFm 0.05% 7 min
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid and Dopamine on the Primesep 100 Column
  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration.   Application Columns: Primesep 100 Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep 100, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

HPLC Separation of DOPAC, Homovanillic Acid in Dopamine on the Primesep 200 Column
  Application Notes: Neurotransmitters DOPAC, homovanillic acid and dopamine were separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns. The method can be used for quantification of neurotransmitters with LC/MS compatible conditions. The compounds are retained by combination of reversed-phase, ion-exchange or ion-exclusion mechanisms. The retention time and selectivity of separation can be adjusted by variation of amount of acetonitrile, buffer pH and buffer concentration. Application Columns: Primesep 100, Primesep 200 Application compounds: Dopac, Homovanillic Acid, Dopamine Detection technique: UV, LC/MS, ELSD/CAD

Condition

Column Primesep 200, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFm pH 3.0- 30 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm
 

Description

Class of Compounds Drug, Acid, Monocarboxylic acid,  Hydrophilic, Ionizable, Hormone
Analyzing Compounds DOPAC, Homovanillic Acid, Dopamine
 

Application Analytes:

DOPAC (Dihydroxyphenylacetic Acid)
Dopamine
Homovanillic Acid

Generic Screening Method for Complex Mixtures

Condition

Column Primesep 200, 4.6*150 mm 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 215 nm
 

Description

Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Hormone
Analyzing Compounds Uracil, Epinephrine, DOPA, 2,6-Lutidine, Benzylamine, Hydroxytrypthophan, Homovanillic acid, Phenol, Tryptophan , 2,3-DHBA, Benzoic acid, Methylparaben, Ethylparaben, Toluene, Amitriptyline
 

Application Analytes:

2,3-Dihydroxybenzoic Acid
2,6-Lutidine
Amitriptyline
Benzoic Acid
Benzylamine
DOPA (3,4-dihydroxy-L-phenylalanine)
Epinephrine
Ethyl Paraben
Homovanillic Acid
Hydroxytryptophan
Methylparaben
Phenol
Toluene
Tryptophan
Uracil