Hydrocortisone

CAS Number	50-23-7
Molecular Formula	C21H30O5
Molecular Weight	362.467 g/mol
InChI Key	JYGXADMDTFJGBT-VWUMJDOOSA-N
LogP	1.61
Synonyms	Hydrocortisone (11beta)-11,17,21-Trihydroxypregn-4-ene-3,20-dione Pregn-4-ene-3,20-dione, 11,17,21-trihydroxy-, (11beta)- 50-23-7 Cortisol CORT Pregn-4-ene-3,20-dione, 11,17,21-trihydroxy-, (11β)- (11β)-11,17,21-Trihydroxypregn-4-ene-3,20-dione 11β,17,21-Trihydroxypregn-4-ene-3,20-dione 11β,17,21-Trihydroxyprogesterone 11β,17α,21-Trihydroxypregn-4-ene-3,20-dione 11β-Hydroxycortisone 17-Hydroxycorticosterone 17α-Hydroxycorticosterone 4-Pregnene-11β,17α,21-triol-3,20-dione Acticort Aeroseb HC Ala-Cort Anti-inflammatory hormone CaldeCort Spray Cetacort Cobadex Cortanal Cort-Dome Cortenema Corticreme Cortifan Cortiment Cortispray Cortonema Cortril Dermacort Dermocortal Dermolate Dihydrocostisone Dioderm Domolene-HC Efcorbin Efcortelan Eldecort Epiderm H Esiderm H Evacort Ficortril Genacort Heb-Cort Hidro-Colisona hidrocortisona Hycortol Hycortole Hydracort Hydrasson Hydro-Adreson Hydrocort Hydrocortal Hydrocorticosterone Hydrocortison Hydrocortisyl Hydrocortone Hydroskin Hytone lotion Incortin-H Kendall's compound F Lacticare HC Medicort Mildison NSC 10483 Nutracort Otosone-F Penecort Permicort Polcort H Reichstein's substance M Scheroson F Synacort Tarcortin Texacort Texacort lotion 25 Timocort Traumaide Zenoxone EINECS 200-020-1 Eldercort 11-beta-Hydrocortisone 17alpha-Hydroxycorticosterone Pregn-4-ene-3,20-dione, 11beta,17,21-trihydroxy- Pregn-4-ene-3,20-dione, 11,17,21-trihydroxy-, (11-beta)- 4-Pregnene-11beta,17alpha,21-triol-3,20-dione 11beta,17,21-Trihydroxypregn-4-ene-3,20-dione Algicirtis Dermaspray Remederm HC Colocort Balneol-HC Beta-HC Flexicort Glycort Hydrocortisone in absorbase Hydro-RX Nogenic HC Stie-Cort Anucort Anucort-HC Proctozone HC Rectasol-HC Scalp-Cort UNII-WI4X0X7BPJ Proctosol-HC (11beta)-11,17,21-trihydroxypregn-4-ene-3,20-dione (1S,2R,10S,11S,14S,15S,17S)-14,17-dihydroxy-14-(2-hydroxyacetyl)-2,15-dimethyltetracyclo[8.7.0.02,7.011,15]heptadec-6-en-5-one 11-Hydrocortisone 11-beta-Hydrocortisone 11-beta-Hydroxycortisone 11a-Hydroxycorticosterone 11alpha-Hydroxycorticosterone 11b,17,21-Trihydroxyprogesterone 11b-Hydrocortisone 11b-Hydroxycortisone 11beta,17,21-Trihydroxyprogesterone 11beta,17alpha,21-trihydroxy-4-pregnene-3,20-dione 11beta-Hydroxycortisone 11beta-hydrocortisone 17a-Hydroxycorticosterone 17alpha-Hydroxycorticosterone 4-Pregnene-11alpha,21-triol 3,20-dione 4-Pregnene-11b,17a,21-triol-3,20-dione 4-pregnen-11beta,17alpha,21-triol-3,20-dione Aeroseb-HC Ala-Scalp Alacort Algicirtis Alphaderm Amberin Anflam Aquacort Aquanil HC Barseb HC Basan-Corti Chronocort Clear aid Cleiton Compound F Cor-Tar-Quin Cort-Quin Cortesal Cortifoam Cortisol alcohol Cortisolonum Cortisporin Cortisporin Otico Cortizol Cortolotion Cortonema Cortoxide Cremesone Cremicort-H Cutisol Delacort Derm-Aid Dermil Dome-cort Drotic Ef corlin Efcortelin Eldercort Epicort Fiocortril Foille Insetti Gyno-Cortisone H-Cort HC Heb Cort Hidalone Hycort Hycortol Hydro-colisona Hydrocortisone alcohol Hydrocortisone base Hydrocortisone free alcohol Hydrocortisonum Hydrocortistab Hydroxycortisone Hysone Hytisone Hytone Idrocortisone Incortin-hydrogen Komed HC Kyypakkaus Lacticare-HC Lactisona Lubricort Maintasone Meusicort Milliderm Neo-Cort-Dome Neosporin-H Ear Nystaform-HC Optef Otalgine Otic-Neo-Cort-Dome Otobiotic Otocort Pediotic Suspension Preparation H Hydrocortisone Cream Prepcort Prestwick_265 Prevex HC Proctocort Proctofoam Protocort Racet Rectoid Remederm HC Sanatison Scalpicin Capilar Schericur Sigmacort Signef Stiefcorcil Systral Hydrocort Topicort Transderma H Uniderm Vioform-Hydrocortisone VoSol HC Vytone 1050676-88-4 8056-08-4 80562-38-5 8063-42-1

Applications:

HPLC Separation of Hydrocortisone and Hydrocortisone's Degradation Products

Cortisol (hydrocortisone) is a steroid hormone produced by the adrenal gland. Hydrocortisone is a hydrophobic neutral compound with some polar groups in its structure. Several Primesep mixed-mode columns were used for separation of hydrocortisone and its degradation products. Each column showed a different selectivity for separation, which attributed to different polar-embedded groups on Primesep 100 and Primesep D columns. Both columns can be used for degradation studies of various hydrophobic compounds.

HPLC Method for Analysis of Hydrocortisone
Hydrocortisone
Hydrocortisone

USP Methods for the Analysis of Hydrocortisone on a Legacy L1 Column

Application Notes: Hydrocortisone is a naturally occurring hormone released during times of stress. Hydrocortisone has been synthesized and is used to treat diseases such as allergic reactions and skin conditions. According to the USP methods, hydrocortisone contains not less than 97 percent and not more than 102 percent of hydrocortisone calculated on the dried basis. The USP HPLC method for the separation of hydrocortisone was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A.

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Hydrocortisone

Mobile phase: MeCN/H2O 25:75

Detection technique: UV

Reference: USP30: NF35

Hydrocortisone

HPLC Ananlysis of Hydrocortisone

Hydrocortisone is the drug form of the steroid hormone cortisol. Cortisol is an important biological molecule which affects blood sugar, metabolism and the immune system. It was retained on the reverse-phase column Legacy L1. Comparisons of Legacy L1 and Phenomenex columns are available by request.

Hydrocortisone

HPLC Method for Analysis of Hydrocortisone

Hydrocortisone, also known as 11β,17α,21-trihydroxypregn-4-ene-3,20-dione, is a corticosteroid, is similar to a natural hormone produced by your adrenal glands. It is used to treat certain forms of arthritis as well various skin conditions (redness, swelling, and itching). Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Hydrocortisone. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

HPLC Ananlysis of Hydrocortisone
HPLC Method for Analysis of Hydrocortisone
HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products
Hydrocortisone