Isoleucine

CAS Number 73-32-5
Molecular Formula C6H13NO2
Molecular Weight 131.176 g/mol
InChI Key AGPKZVBTJJNPAG-WHFBIAKZSA-N
LogP -1.72
Synonyms
  • L-Isoleucine
  • 73-32-5
  • (2S,3S)-2-Amino-3-methylpentanoic acid
  • (2S,3S)-α-Amino-β-methyl-n-valeric acid
  • (2S,3S)-α-Amino-β-methylvaleric acid
  • (S)-Isoleucine
  • (S,S)-Isoleucine
  • [S-(R*,R*)]-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvaleric acid
  • 2S,3S-Isoleucine
  • erythro-L-Isoleucine
  • Isoleucine
  • ISOLEUCINE, L-
  • L-(+)-Isoleucine
  • L-Isoleucin
  • L-isoleucina
  • L-Norvaline, 3-methyl-, erythro-
  • NSC 46708
  • Pentanoic acid, 2-amino-3-methyl-, (2S,3S)-
  • Secretory protein (human clone HCEEE79 precursor)
  • Acetic acid, amino-sec-butyl-
  • Acetic acid, amino(1-methylpropyl)-, (R*,R*)-
  • (S-(R*,R*))-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylpentanoic acid, (S-(R*,R*))-
  • EINECS 200-798-2
  • Norvaline, 3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-, (S-(R*,R))-
  • Valeric acid, 2-amino-3-methyl-
  • Isoleucina
  • Isoleucinum
  • UNII-04Y7590D77
  • (2S,3S)-2-Amino-3-methylpentanoate
  • (2S,3S)-2-amino-3-methyl-Pentanoate
  • (2S,3S)-2-amino-3-methyl-pentanoic acid
  • (2S,3S)-a-Amino-b-methyl-N-valerate
  • (2S,3S)-a-Amino-b-methyl-N-valeric acid
  • (2S,3S)-a-Amino-b-methylvalerate
  • (2S,3S)-a-Amino-b-methylvaleric acid
  • (2S,3S)-alph-Amino-beta-methylvalerate
  • (2S,3S)-alph-Amino-beta-methylvaleric acid
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-merthylvalerate
  • (2S,3S)-alpha-Amino-beta-merthylvaleric acid
  • (2S,3S)-alpha-Amino-beta-methyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-methyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-methylvalerate
  • (2S,3S)-alpha-Amino-beta-methylvaleric acid
  • 2-Amino-3-methylpentanoate
  • 2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvalerate
  • Iso-leucine
  • L-Ile
  • [S-(R*,R*)]-2-Amino-3-methylpentanoate
  • [S-(R*,R*)]-2-Amino-3-methylpentanoic acid
  • alpha-amino-beta-methylvaleric acid
  • (2S,3S)-2-ammonio-3-methylpentanoate
  • L-isoleucine zwitterion
  • 1050482-26-2
  • 1131911-83-5
  • 1186126-44-2
  • 410078-51-2
  • 501028-75-7
  • 7004-09-3
  • 807611-99-0
  • 959215-79-3

Applications:


Amino Acids Analysis in Acid Gradient Condition



11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).



Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine

HPLC Separation of Amino Acids in Supplements Capsules



Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.



Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine

HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column

Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.



Application Analytes:

Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine

Separation of allo-D-isoleucine and L- Isoleucine


Application Analytes:

Isoleucine
L-Isoleucine
allo-D-isoleucine

HPLC Separation of Mixture of Essential Amino Acids


Application Analytes:

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
Methionine
Phenylalanine
Tryptophan
Valine

HPLC Separation of Mixture of 12 Amino Acids


Application Analytes:

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
Methionine
Phenylalanine
Proline
Tryptophan
Valine