Isoleucine

Isoleucine structural formula

CAS Number 73-32-5
Molecular Formula C6H13NO2
Molecular Weight 131.176 g/mol
InChI Key AGPKZVBTJJNPAG-WHFBIAKZSA-N
LogP -1.72
Synonyms
  • L-Isoleucine
  • 73-32-5
  • (2S,3S)-2-Amino-3-methylpentanoic acid
  • (2S,3S)-α-Amino-β-methyl-n-valeric acid
  • (2S,3S)-α-Amino-β-methylvaleric acid
  • (S)-Isoleucine
  • (S,S)-Isoleucine
  • [S-(R*,R*)]-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvaleric acid
  • 2S,3S-Isoleucine
  • erythro-L-Isoleucine
  • Isoleucine
  • ISOLEUCINE, L-
  • L-(+)-Isoleucine
  • L-Isoleucin
  • L-isoleucina
  • L-Norvaline, 3-methyl-, erythro-
  • NSC 46708
  • Pentanoic acid, 2-amino-3-methyl-, (2S,3S)-
  • Secretory protein (human clone HCEEE79 precursor)
  • Acetic acid, amino-sec-butyl-
  • Acetic acid, amino(1-methylpropyl)-, (R*,R*)-
  • (S-(R*,R*))-2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylpentanoic acid, (S-(R*,R*))-
  • EINECS 200-798-2
  • Norvaline, 3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-
  • Pentanoic acid, 2-amino-3-methyl-, (S-(R*,R))-
  • Valeric acid, 2-amino-3-methyl-
  • Isoleucina
  • Isoleucinum
  • UNII-04Y7590D77
  • (2S,3S)-2-Amino-3-methylpentanoate
  • (2S,3S)-2-amino-3-methyl-Pentanoate
  • (2S,3S)-2-amino-3-methyl-pentanoic acid
  • (2S,3S)-a-Amino-b-methyl-N-valerate
  • (2S,3S)-a-Amino-b-methyl-N-valeric acid
  • (2S,3S)-a-Amino-b-methylvalerate
  • (2S,3S)-a-Amino-b-methylvaleric acid
  • (2S,3S)-alph-Amino-beta-methylvalerate
  • (2S,3S)-alph-Amino-beta-methylvaleric acid
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-merthyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-merthylvalerate
  • (2S,3S)-alpha-Amino-beta-merthylvaleric acid
  • (2S,3S)-alpha-Amino-beta-methyl-N-valerate
  • (2S,3S)-alpha-Amino-beta-methyl-N-valeric acid
  • (2S,3S)-alpha-Amino-beta-methylvalerate
  • (2S,3S)-alpha-Amino-beta-methylvaleric acid
  • 2-Amino-3-methylpentanoate
  • 2-Amino-3-methylpentanoic acid
  • 2-Amino-3-methylvalerate
  • Iso-leucine
  • L-Ile
  • [S-(R*,R*)]-2-Amino-3-methylpentanoate
  • [S-(R*,R*)]-2-Amino-3-methylpentanoic acid
  • alpha-amino-beta-methylvaleric acid
  • (2S,3S)-2-ammonio-3-methylpentanoate
  • L-isoleucine zwitterion
  • 1050482-26-2
  • 1131911-83-5
  • 1186126-44-2
  • 410078-51-2
  • 501028-75-7
  • 7004-09-3
  • 807611-99-0
  • 959215-79-3

Applications:

Amino Acids Analysis in Acid Gradient Condition
11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD).

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer TFA , gradient  0.05-0.3 % , 25 min
Flow Rate 1.0 ml/min
Detection ELSD
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Gaspartic acid, Glutamic acid, Alanine, Valine, Methionine, Isoleucine, Cysteine, Phenylalanine, Histidine, Lysine, Arginine
 
Application Analytes:

Alanine
Amino Acids
Arginine
Aspartic Acid
Cysteine
Glutamic Acid
Histidine
Isoleucine
Lysine
Methionine
Phenylalanine
Valine
HPLC Separation of Amino Acids in Supplements Capsules
Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 30/70%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, Glutamine,  L-Valine (Val/V),  L-Isoleucine (Ile/I), L-Leucine (Leu/L)
 
Application Analytes:

Arginine
Glutamine
Isoleucine
Leucine
Valine
HPLC Separation of Amino Acids in Zero Organic Mode on Primesep 200 column
Essential and non-essential amino acids can be retained and separated in zero-organic mode on Primesep mixed-mode HPLC columns. Zero-organic mode is required to monitor isotopes of carbon. Amino acids are retained by combination of reversed-phase and cation-exchange mechanisms. At lower pH, some of the amino acids are more hydrophobic. Buffer pH will affect ionization state of amino acids, and at higher pH (above 2.5), the amino acids will be less hydrophobic and retentive in zero-organic mode. Amino acids can be monitored by low UV. Method can be used in archeological research for analysis of various molecules where presence of organic component of the mobile phase interferes with analysis.

Condition
Column Primesep 200, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Na2HPO4
Flow Rate 1.0 ml/min
Detection UV 210 nm
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Valine, Leucyne, Isoleycine, Tyrosine, Phenylalanine, Histidine, Lysine
 
Application Analytes:

Histidine
Isoleucine
Leucine
Lysine
Phenylalanine
Tyrosine
Valine
Separation of allo-D-isoleucine and L- Isoleucine

Condition
Column Primesep 100, Primesep 200 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, FA
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine
 
Application Analytes:

Isoleucine
L-Isoleucine
allo-D-isoleucine
HPLC Separation of Mixture of Essential Amino Acids on Primesep 100 Column
 

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 25/75%
Buffer Gradient H2SO4 0.05-0.2% 25 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), L- Tryptophan (Trp/W)
 
Application Analytes:

Amino Acids
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Histidine hydrochloride monohydrate
L-Isoleucine
L-Methionine
L-Threonine
Methionine
Phenylalanine
Tryptophan
Valine
HPLC Separation of Mixture of 12 Amino Acids on Primesep 100 Column
 

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer H2SO4 0.05% 12 min hold, gradient 0.05-0.20, 13 min
Flow Rate 1.0 ml/min
Detection UV, 200 nm
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Asparagine (Asn/N), L-Threonine (Thr/T), L-Cysteine (Cys/C), L-Proline (Pro/P), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L- Tryptophan (Trp/W), L-Histidine (His/H), L-Arginine (Arg/R)
 
Application Analytes:

Amino Acids
Arginine
Asparagine
Cysteine
D-Isoleucine
D-Leucine
D-Valine
DL-Isoleucine
Histidine
Isoleucine
L-Cysteine
L-Methionine
L-Threonine
Leucine
Methionine
Phenylalanine
Proline
Tryptophan
Valine
HPLC Separation of Allo-Isoleucine, Isoleucine, Leucine on Primesep 100 Column
HPLC.cloud View on hplc.cloud HPLC Separation of Allo-Isoleucine Isoleucine, Leucine on Primesep 100 Column Chr_1197

Condition
Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmFM pH 3.0 - 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Allo-Isoleucine, Isoleucine, Leucine
 
Application Analytes:

D-Isoleucine
D-Leucine
DL-Isoleucine
Isoleucine
L-Alloisoleucine
L-Isoleucine
Leucine
allo-D-isoleucine
HPLC Separation of Mixture of Nine Essential Amino acids and Arginine on Newcrom AH Column
HPLC.cloud View on hplc.cloud Essential amino acids cannot be made by the body. As a result, they must come from food. The 9 essential amino acids are: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine.

Condition
Column Newcrom AH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 5%
Buffer Gradient Formic Acid - 3-9%, 20 min
Flow Rate 1.0 ml/min
Detection CAD
 

Description
Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds L-Threonine (Thr/T), L-Valine (Val/V), DL-Methionine (Met/M), L-Isoleucine (Ile/I), L-Leucine (Leu/L), L-Phenylalanine (Phe/F), L-Histidine (His/H), Lysine (Lys/K) , L- Tryptophan (Trp/W), L-Arginine (Arg/R)
 
Application Analytes:

Arginine
Histidine
Isoleucine
L-Threonine
Leucine
Lysine
Methionine
Phenylalanine
Tryptophan
Valine